3.2.2.4: AMP nucleosidase
This is an abbreviated version!
For detailed information about AMP nucleosidase, go to the full flat file.
Word Map on EC 3.2.2.4
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3.2.2.4
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vinelandii
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azotobacter
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deaminase
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formycin
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ribose
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5-phosphate
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synthesis
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5'-monophosphate
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schramm
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hypoxanthine
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transition-state
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n-glycosidic
- 3.2.2.4
- vinelandii
- azotobacter
- deaminase
- formycin
- ribose
- 5-phosphate
- synthesis
- 5'-monophosphate
-
schramm
- hypoxanthine
-
transition-state
-
n-glycosidic
Reaction
Synonyms
adenosine monophosphate nucleosidase, adenylate nucleosidase, AMN, AMP ribosidase, More
ECTree
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Crystallization
Crystallization on EC 3.2.2.4 - AMP nucleosidase
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purified recombinant enzyme bound to formycin 5'-phosphate or phosphate, hanging drop vapour diffusion method, 0.002 ml of 15-20 mg/ml protein in 10 mM Tris-HCl, pH 7.6, 0.15 M NaCl, 2 mM MgCl2, and 1 mM DTT, is mixed with an equal amount of reservoir solution containing 1.8-2.0 M ammonium formate, 0.1 M HEPES, pH 6.8-7.2, 0.2 M NaCl, and 1 mm DTT, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.6-3.0 A resolution
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