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3.2.1.94: glucan 1,6-alpha-isomaltosidase

This is an abbreviated version!
For detailed information about glucan 1,6-alpha-isomaltosidase, go to the full flat file.

Word Map on EC 3.2.1.94

Reaction

isomaltotriose
+
H2O
=
alpha-isomaltose
 +
D-glucose

Synonyms

AgIMD, dextranase, isomalto-, exo-isomalto-hydrolase, Exo-isomaltohydrolase, G2-dextranase, Glucan 1,6-alpha-isomaltosidase, IMD, isomalto-dextranase, isomaltodextranase, isomaltohydrolase, exo

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.94 glucan 1,6-alpha-isomaltosidase

Engineering

Engineering on EC 3.2.1.94 - glucan 1,6-alpha-isomaltosidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D107A
-
activity similar to wild-type enzyme
D163A
-
activity similar to wild-type enzyme
D198N
-
0.003% of wild-type activity, largely increased Kd-value
D207A
4fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 28fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
D207A/N209A
4.2fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 17.8fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
D227A
-
no activity of dextran hydrolysis
D227E
-
decreased activity of dextran hydrolysis
D227N
-
decreased activity of dextran hydrolysis
D227Q
-
decreased activity of dextran hydrolysis
D266N
-
0.011% of wild-type activity, without change in substrate binding ability
D295A
-
activity similar to wild-type enzyme
D313N
-
0.002% of wild-type activity, largely increased Kd-value
D340A
-
activity similar to wild-type enzyme
D342A
-
no activity of dextran hydrolysis
D342E
-
decreased activity of dextran hydrolysis
D342N
-
decreased activity of dextran hydrolysis
D342Q
-
decreased activity of dextran hydrolysis
D373A
-
activity similar to wild-type enzyme
D396A
-
activity similar to wild-type enzyme
E420A
-
activity similar to wild-type enzyme
M243A
233fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 334fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
D207A
-
4fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 28fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
-
D207A/N209A
-
4.2fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 17.8fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
-
M243A
-
233fold decrease in kcat/Km for 4-nitrophenyl alpha-isomaltoside as compared to wild-type value. 334fold decrease in kcat/Km for dextran T2000 as compared to wild-type value
-