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3.2.1.78: mannan endo-1,4-beta-mannosidase

This is an abbreviated version!
For detailed information about mannan endo-1,4-beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.78

Reaction

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
+
H2O
=
Manbeta(1-4)Manbeta(1-4)Man
 +
Manbeta(1-4)Man

Synonyms

(1,4)-beta-D-mannan mannanohydrolase, 1,4-beta-D-mannan mannanohydrolase, 1,4-beta-mannanase, beta-1,4-D-mannanase, beta-1,4-mannan 4-mannanohydrolase, beta-1,4-mannanase, beta-D-mannanase, Beta-mannanase, beta-mannanase B, CaMan, CelB, cold-adapted beta-mannanase, CsMan5, CtManf, Dtur_0671, em26a, endo-1,4-beta-D-mannanase, endo-1,4-beta-mannanase, endo-1,4-mannanase, endo-acting beta-1,4-mannanase, endo-beta 1,4-mannanase, endo-beta-(1,4)-mannanase, endo-beta-(1->4)-mannanase, endo-beta-1,4 mannanase, endo-beta-1,4,D-mannanase, endo-beta-1,4-D-mannanase, endo-beta-1,4-mannanase, endo-beta-1,4-mannase, endo-beta-D-1,4-mannanase, endo-beta-D-mannanase, endo-beta-mannanase, endo-mannanase, GH 134 beta-1,4-mannanase, GH134, GH5-CBM27, KMAN-3, LeMAN4, LeMAN4a, Man, MAN I, Man II, MAN-P, Man1, Man113A, Man134A, Man26A, Man26A-50K, Man26b, Man4A, Man5, Man5A, Man5C, man5D, Man5P1, Man5XZ3, Man5_8, Man7, ManA, ManA/HmA, ManB, ManB-1601, ManC, ManEM17, ManF, ManH, ManIII, mannan endo-1,4-beta-mannanase, mannan endo-1,4-beta-mannosidase, mannan endo-1.4-beta-D-mannosidase, mannanase, mannanase, endo-1,4-beta-, ManP, ManS2, MYCTH_99077, PoMan5A, Rman, TpMan, TrMan5A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.78 mannan endo-1,4-beta-mannosidase

Specific Activity

Specific Activity on EC 3.2.1.78 - mannan endo-1,4-beta-mannosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3
crude enzyme, at pH 6.0 and 60°C
0.34
-
crude extract, at pH 5.0 and 40°C
0.475
-
crude extract, at pH 5.0 and 50°C
0.69
-
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man}
1.13
-
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
1.95
-
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
10760
-
purified recombinant enzyme, pH 7.0, 55°C, substrate locust bean gum
11.48
-
pH 7.0, 30°C, substrate galactomannan
1139
-
pH 5.0, 40°C
11900
-
after 51fold purification, at pH 4.0 and 40°C
12.2
-
substrate locust bean gum, pH 7.0, 40°C
129.3
-
pH 4.0, 40°C
14
after 46.7fold purification, at pH 6.0 and 60°C
1400
-
ManB, 37°C, pH 5.0
14710
substrate ivory nut mannan, pH 6.0, 50°C
1672
50°C, pH 6.0
1703
-
purified recombinant enzyme, substrate locust bean gum, pH 4.0, 80°C
18.5
-
substrate glucomannan, pH 7.0, 40°C
182
substrate beta-mannan, pH 5.0, 60°C
190
-
isozyme MAN I, mannan A
2.2
-
substrate guar gum, pH 7.0, 40°C
220
substrate carob galactomannan, pH 5.0, 60°C
2223
pH 6.0, 60°C, substrate 1,4-beta-D-mannan
225.4
-
isozyme MAN II, after 49fold purification with Phenyl Sepharose chromatography
236
Reinekea sp. KIT-YO10
-
pH 8.0, 40°C, substrate konjac glucomannan
240
-
crude lysate, at pH 4.0 and 40°C
264.1
pH 5.0, 50°C, native protein
27.4
-
pH 7.0, 30°C
2718
-
purified recombinant enzyme, pH 6.5, 45°C
294
-
isozyme MAN I, guar gum
3.1
-
Manbeta(1-4)Manbeta(1-4)Man
312
substrate konjac glucomannan, pH 5.0, 60°C
317
-
isozyme MAN II, guar gum
3373
pH 1.5, 65°C
380
-
41.9 kDa protein
4.6
-
crude medium
4.8
-
pH 7.0, 30°C, substrate beta-1,4-mannan
416.3
30°C, pH 3.5
420.9
-
purified recombinant enzyme, locust bean gum as substrate, pH 4.0, 80°C
4449
purified recombinant enzyme, pH 6.3, 70°C
45.7
-
isozyme MAN I, after 9.9fold purification with DEAE Sepharose chromatography
47.5
purified recombinant enzyme, pH 4.5, 50°C
471
-
isozyme MAN I, after 102.4fold purification with Phenyl Sepharose chromatography
475
-
61.2 kDa protein
4839
pH 7.6, 60°C
56.7
-
isozyme MAN II, after 12.3fold purification with DEAE Sepharose chromatography
562
-
isozyme MAN I, locust bean gum
627
-
isozyme MAN II, locust bean gum
63.94
-
after 188.07fold purification, at pH 5.0 and 40°C
635
pH 6.0, 60°C, substrate locust bean gum
64.6
pH 5.0, 50°C, recombinant protein
663
-
isozyme MAN I, low viscosity locust bean galactomannan
751
-
isozyme MAN II, low viscosity locust bean galactomannan
768.5 - 1249
purified recombinant chimeric enzymes, pH 5.0, 50°C
83.81
-
after 178.4fold purification, at pH 5.0 and 50°C
8302
-
pH 6.0, 50°C
8498
substrate locust bean gum, pH 6.0, 50°C
9.6
-
pH 7.0, 30°C, substrate glucomannan
928
-
pH 6.4, 50°C
962
pH 6.0, 60°C, substrate glucomannan
additional information