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3.2.1.78: mannan endo-1,4-beta-mannosidase

This is an abbreviated version!
For detailed information about mannan endo-1,4-beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.78

Reaction

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
+
H2O
=
Manbeta(1-4)Manbeta(1-4)Man
 +
Manbeta(1-4)Man

Synonyms

(1,4)-beta-D-mannan mannanohydrolase, 1,4-beta-D-mannan mannanohydrolase, 1,4-beta-mannanase, beta-1,4-D-mannanase, beta-1,4-mannan 4-mannanohydrolase, beta-1,4-mannanase, beta-D-mannanase, Beta-mannanase, beta-mannanase B, CaMan, CelB, cold-adapted beta-mannanase, CsMan5, CtManf, Dtur_0671, em26a, endo-1,4-beta-D-mannanase, endo-1,4-beta-mannanase, endo-1,4-mannanase, endo-acting beta-1,4-mannanase, endo-beta 1,4-mannanase, endo-beta-(1,4)-mannanase, endo-beta-(1->4)-mannanase, endo-beta-1,4 mannanase, endo-beta-1,4,D-mannanase, endo-beta-1,4-D-mannanase, endo-beta-1,4-mannanase, endo-beta-1,4-mannase, endo-beta-D-1,4-mannanase, endo-beta-D-mannanase, endo-beta-mannanase, endo-mannanase, GH 134 beta-1,4-mannanase, GH134, GH5-CBM27, KMAN-3, LeMAN4, LeMAN4a, Man, MAN I, Man II, MAN-P, Man1, Man113A, Man134A, Man26A, Man26A-50K, Man26b, Man4A, Man5, Man5A, Man5C, man5D, Man5P1, Man5XZ3, Man5_8, Man7, ManA, ManA/HmA, ManB, ManB-1601, ManC, ManEM17, ManF, ManH, ManIII, mannan endo-1,4-beta-mannanase, mannan endo-1,4-beta-mannosidase, mannan endo-1.4-beta-D-mannosidase, mannanase, mannanase, endo-1,4-beta-, ManP, ManS2, MYCTH_99077, PoMan5A, Rman, TpMan, TrMan5A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.78 mannan endo-1,4-beta-mannosidase

Purification

Purification on EC 3.2.1.78 - mannan endo-1,4-beta-mannosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE column chromatography and Sephadex G75 gel filtration
-
ammonium sulfate precipitation, DEAE-cellulose column chromatography and Sephacryl S200 gel filtration
-
ammonium sulfate precipitation, DEAE-Sephadex column chromatography and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, DEAE-Sephadex column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Q-Sepharose column chromatography
-
ammonium sulfate precipitation, HisTrap column chromatography, HiTrap DEAE column chromatography, and Superdex 75 gel filtration
both native protein and expressed in Saccharomyces cerevisiae
butyl-Toyopearl 650 M column chromatography, DEAE-Toyopearl 650 M column chromatography, Mono Q column chromatography, Mono S column chromatography, Superdex 75 gel filtration, and HisTrap column chromatography
cation exchange chromatography
CM-Sepharose resin column chromatography and Superdex 200 gel filtration
-
fast flow column chromatography
-
four enzyme forms
-
from culture supernatant
-
from digestive fluid
-
HisTrap column chromatography and Superdex G75 gel filtration
-
HiTrap Q column chromatography and Superose 6 column chromatography
-
M-I, M-II, M-III
-
native enzyme 29.4fold by anion exchange chromatography
Reinekea sp. KIT-YO10
-
native enzyme by ultrafiltration and anion exchange chromatography
Ni-NTA agarose resin column chromatography
-
Ni-NTA column chromatography
-
Ni-NTA resin column chromatography
Ni2+-NTA column chromatography
nickel-nitrilotriacetic acid agarose column chromatography and HiTrap Q column chromatography
-
phenyl Sepharose column chromatography
purification from seedling
recombinant chimeric enzymes from Pichia pastoris strain X33 culture supernatant by ammonium sulfate fractionation and gel filtration
recombinant enzyme
recombinant enzyme 9.7fold from Pichia pastoris strain GS115 culture supernatant to homogeneity by ammonium sulfate fractionation, dialysis, PEG 20000 fractionation, anion exchange chromatography, ultrafiltration, and gel filtration
recombinant enzyme from Pichia pastoris strain GS115
-
recombinant extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by ultrafiltration
recombinant extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by ultrafiltration and anion exchange chromatography
-
recombinant extracellular enzyme from Yarrowia lipolytica culture supernatant by ultrafiltration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
-
recombinant His-tagged full-length enzyme and catalytic domain by nickel affinity chromatography
recombinant His-tagged full-length enzyme and isolated catalytic domain from Escerichia coli strain BL21(DE3)DELTASlyD by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His6-tagged extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by nickel affinity chromatography to homogeneity
-
recombinant protein
recombinant protein containing active protein domain
-
recombinant protein expressed in Aspergillus oryzae
-
recombinant protein expressed in Escherichia coli
-
recombinant wild-type and mutant enzymes from Pichia pastoris strain GS115 culture supernatant by ultrafiltration and anion exchange chromatography to homogeneity
-
salt extraction, dialysis, cation exchange chromatography
-
TALON metal affinity resin column chromatography