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0.00066 - 0.04
2,4-dinitrophenyl-beta-mannobioside
0.25 - 0.46
4-methylumbelliferyl mannotrioside
0.592 - 1.3
locust bean gum
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3.07 - 3.12
low viscosity locust bean galactomannan
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1.6 - 2.9
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
0.6 - 1.8
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
9
Manbeta(1-4)Manbeta(1-4)Man
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2.49
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
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1.61 - 5.3
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
0.5
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
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0.31 - 10.6
Mannotetraose
additional information
carob galactomannan
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0.00066
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant E212A
0.00074
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant Y285A
0.0014
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant D283H
0.0037
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant H211A/D283A
0.0068
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant H211A
0.0093
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, wild type
0.01
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant H211N
0.0141
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant W162A
0.0149
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant W156A
0.0166
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant D283A
0.0205
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant W217A
0.04
2,4-dinitrophenyl-beta-mannobioside
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pH 6.5, mutant H143A
0.25
4-methylumbelliferyl mannotrioside
pH 40°C, pH 4.5, wild-type enzyme
0.46
4-methylumbelliferyl mannotrioside
pH 40°C, pH 4.5, mutant enzyme R171K
0.592
locust bean gum
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pH and temperature not specified in the publication
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0.6
locust bean gum
pH 40°C, pH 4.5, wild-type enzyme
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1.3
locust bean gum
pH 40°C, pH 4.5, mutant enzyme R171K
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3.07
low viscosity locust bean galactomannan
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isozyme MAN I
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3.12
low viscosity locust bean galactomannan
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isozyme MAN II
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1.6
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
mutant W283S, pH 5.5, 37°C
1.8
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
wild-type, pH 5.5, 37°C
2.8
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
mutant W283S, pH 5.5, 37°C
2.9
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
pH 5.5, 37°C
0.6
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
wild-type, pH 5.5, 37°C
1
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
mutant W283S, pH 5.5, 37°C
1.3
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
mutant W283S, pH 5.5, 37°C
1.8
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
pH 5.5, 37°C
1.61
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
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5.3
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
30°C, pH 4.8
0.05
mannohexaose
pH 40°C, pH 4.5, wild-type enzyme
0.34
mannohexaose
pH 40°C, pH 4.5, mutant enzyme R171K
0.08
Mannopentaose
pH 40°C, pH 4.5, wild-type enzyme
1.7
Mannopentaose
pH 40°C, pH 4.5, mutant enzyme R171K
0.31
Mannotetraose
pH 40°C, pH 4.5, wild-type enzyme
10.6
Mannotetraose
pH 40°C, pH 4.5, mutant enzyme R171K
additional information
carob galactomannan
Km value 2.22 mg/ml, pH 5.0, 60°C
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additional information
carob galactomannan
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Km value 2.22 mg/ml, pH 5.0, 60°C
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additional information
konjac glucomannan
Km value 3.22 mg/ml, pH 5.0, 60°C
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additional information
konjac glucomannan
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Km value 3.22 mg/ml, pH 5.0, 60°C
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additional information
konjac glucomannan
Km value 4.7 mg/ml, pH 5.4, 70°C
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additional information
konjac glucomannan
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Km value 4.7 mg/ml, pH 5.4, 70°C
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additional information
locust bean gum
KM value is 3.8 mg/ml
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additional information
locust bean gum
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KM value is 34.9 mg/ml, pH 5.0, 28°C
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copra beta-mannan: 2.0 mg/ml, locust bean beta-mannan: 3.8 mg/ml, konjak beta-mannan: 7.7 mg/ml, carob galactomannan: 0.9 mg/ml
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
apparent Km values of the mannanase for locust bean gum, glucomannan, glactomannan and mannan are 3, 2.3, 1.6 and 0.54 mg/ml, respectively
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additional information
additional information
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apparent Km values of the mannanase for locust bean gum, guar gum and konjac powder are 7.6, 10.5 and 27.4 mg/ml, respectively
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additional information
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Km value for locust bean gum at pH 2.4, 50°C is 0.93 mg/ml
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additional information
additional information
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Km value for locust bean gum at pH 2.4, 50°C is 0.93 mg/ml
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additional information
additional information
Km value is 0.16 mg/ml for locust-bean galactomannan
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additional information
additional information
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Km value is 0.16 mg/ml for locust-bean galactomannan
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additional information
additional information
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Km value for konjac flour is 7.5 mg/ml, for locust bean gum 11.67 mg/ml
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additional information
additional information
Km value for konjac glucomannan 0.6 mg/ml
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additional information
additional information
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Km value for konjac glucomannan 0.6 mg/ml
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additional information
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Km values are 14.9 mg/ml, 17.5 mg/ml, and 15.2 mg/ml for glucomannan, locust bean gum and beta-D-mannan, respectively
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additional information
additional information
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Km values are 14.9 mg/ml, 17.5 mg/ml, and 15.2 mg/ml for glucomannan, locust bean gum and beta-D-mannan, respectively
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additional information
additional information
Km values for konjac glucomannan of low viscosity, locust bean gum galactomannan, carob galactomannan of low viscosity, and 1,4-beta-D-mannan from carob are 0.6 mg/ml, 2.0 mg/ml, 2.2 mg/ml and 1.5 mg/ml, respectively
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additional information
additional information
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Km values for konjac glucomannan of low viscosity, locust bean gum galactomannan, carob galactomannan of low viscosity, and 1,4-beta-D-mannan from carob are 0.6 mg/ml, 2.0 mg/ml, 2.2 mg/ml and 1.5 mg/ml, respectively
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additional information
additional information
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Km values for locust bean gum, guar gum, and copra mannan, are 0.11, 0.28, and 0.33 mg/ml, respectively
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additional information
additional information
kinetics of recombinant chimeric enzymes, overview
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additional information
additional information
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Km is 2.074 mg/ml with guar gum for the recombinant enzyme at pH 5.5 and 30°C
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additional information
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Km of the recombinant enzyme is 7.8 mg/ml for locust bean gum, pH 4.5, 50°C
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additional information
additional information
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Km values of the recombinant enzyme are 3.78 mg/ml for locust bean gum, and 7.75 mg/ml for guar gum
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additional information
additional information
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Km values with ocust bean gum are 0.9 mg/ml for wild-type enzyme, 0.8 mg/ml for mutant Man5DELTACBM, and 1.1 mg/ml for mutant Man5DELTACL
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additional information
additional information
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Michaelis-Menten kinetics, Km values for locust bean gum, konjac mannan, and guar gum are 7.6 mg/ml, 2.1 mg/ml, and 2.3 mg/ml, respectively, pH 4.0, 80°C
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additional information
additional information
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recombinant enzyme, Km for locust bean gum is 0.83 ± 0.2 mg/ml, pH 4.0, 80°C
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