Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.74: glucan 1,4-beta-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.74

Reaction

(Glcbeta(1-4))r
+
H2O
=
(Glcbeta(1-4))r-1
+
beta-D-glucopyranose

Synonyms

1,4-beta-D-glucan glucanohydrolase, 1,4-beta-D-glucan glucohydrolase, b-glucan glucohydrolase, beta-(1-4)-glucanase, beta-1,4-beta-glucanase, beta-1,4-glucosidase, beta-glucosidase, beta-glucosidase/xylosidase, Bgl, BglA, Carboxymethyl cellulase, CbhA, Cel48A, Cel9A, Cel9D, cellobiase, cellobiohydrolase, Cellulase, Cex, endo-1,4-beta-D-glucanase, endo/exocellulase, Ex-1, exo-1,4-beta-glucanase, exo-1,4-beta-glucosidase, exo-beta-1,4-glucanase, exo-beta-1,4-glucosidase, exo-bets-1,4-glucanase, exocellulase, exocellulase I, exoglucanase, family 9 glycoside hydrolase, family-1 glycosyl hydrolase, FiG, GH9, glucan 1,4-beta-glucosidase, glucohydrolase, NA23_08975, P-42, Rubg3A, Rubg3B, Rucel5B, TSC, Xyn10E

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.74 glucan 1,4-beta-glucosidase

Temperature Stability

Temperature Stability on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 40
-
P-42, pH 5.0, retains more than 80% of its maximum activity
25 - 55
-
Ex-1, pH 5.0, retains more than 80% of its maximum activity
30 - 45
-
for 1 h, pH 5.2
40 - 50
decreases markedly above 50ºC
40 - 60
-
loses none of its activity for a period of up to 5 h
50 - 70
-
at 70°C, 65°C, 60°C, and 50°C the enzyme retains 50% of the initial activity after 2, 10, 16, and 35 min, respectively
55
-
stable below 55°C
60
-
the melting temperature of the enzyme is at 60°C
70
-
undergoes considerable deactivation within quite a short time
70 - 90
-
the hydrolyzing activity is not diminished up to 10 days at 70°C or 75°C. The half-lives of the recombinant enzyme are about 200 and 113 h at 80°C and 85°C, respectively. The enzyme activity is drastically reduced at 90°C
80
Thermomonospora sp.
-
half-life: 20 min
95
-
50% activity loss after 30 min at 95°C