Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.74: glucan 1,4-beta-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.74

Reaction

(Glcbeta(1-4))r
+
H2O
=
(Glcbeta(1-4))r-1
 +
beta-D-glucopyranose

Synonyms

1,4-beta-D-glucan glucanohydrolase, 1,4-beta-D-glucan glucohydrolase, b-glucan glucohydrolase, beta-(1-4)-glucanase, beta-1,4-beta-glucanase, beta-1,4-glucosidase, beta-glucosidase, beta-glucosidase/xylosidase, Bgl, BglA, Carboxymethyl cellulase, CbhA, Cel48A, Cel9A, Cel9D, cellobiase, cellobiohydrolase, Cellulase, Cex, endo-1,4-beta-D-glucanase, endo/exocellulase, Ex-1, exo-1,4-beta-glucanase, exo-1,4-beta-glucosidase, exo-beta-1,4-glucanase, exo-beta-1,4-glucosidase, exo-bets-1,4-glucanase, exocellulase, exocellulase I, exoglucanase, family 9 glycoside hydrolase, family-1 glycosyl hydrolase, FiG, GH9, glucan 1,4-beta-glucosidase, glucohydrolase, NA23_08975, P-42, Rubg3A, Rubg3B, Rucel5B, TSC, Xyn10E

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.74 glucan 1,4-beta-glucosidase

Engineering

Engineering on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E795Q
-
inactive enzyme
N44A
-
based on the pUC12-1.1Cex PTIS plasmid
Q87M
-
based on the pUC12-1.1Cex PTIS plasmid
Q87Y
-
based on the pUC12-1.1Cex PTIS plasmid
D166A
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
DELTAS168
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
E612A
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
S168A
retains about 40% of activity compared to the wild-type
A229V
-
loop mutant, weaker binding affinities to all tested ligands
E495D
-
active site mutant, weaker binding affinities to all tested ligands
E495N
-
active site mutant, weaker binding affinities to all tested ligands
G234A
-
loop mutant, weaker binding affinities to all tested ligands
G234D
-
loop mutant, weaker binding affinities to all tested ligands
G234N
-
loop mutant, weaker binding affinities to all tested ligands
G234S
-
loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose
G234S/G284P
-
loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside
G284A
-
loop mutant, weaker binding affinities to all tested ligands
G284I
-
loop mutant, weaker binding affinities to all tested ligands
G284P
-
loop mutant, weaker binding affinities to all tested ligands
G284P/A229V
-
loop mutant, weaker binding affinities to all tested ligands
G331A
-
active site mutant, weaker binding affinities to all tested ligands
G496P
-
loop mutant, weaker binding affinities to all tested ligands
H326A
-
active site mutant, weaker binding affinities to all tested ligands
H326S
-
active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose
L230C
-
loop mutant, weaker binding affinities to all tested ligands
L230C/D512C
-
loop mutant, weaker binding affinities to all tested ligands
N233C
-
loop mutant, weaker binding affinities to all tested ligands
N233C/D506C
-
loop mutant, weaker binding affinities to all tested ligands
W329C
-
active site mutant, weaker binding affinities to all tested ligands
W332A
-
active site mutant, weaker binding affinities to all tested ligands
W332Y
-
active site mutant, weaker binding affinities to all tested ligands
additional information