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3.2.1.73: licheninase

This is an abbreviated version!
For detailed information about licheninase, go to the full flat file.

Word Map on EC 3.2.1.73

Reaction

beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose
+ 4 H2O = 5 beta-D-glucopyranose

Synonyms

(1,3)(1,4)-beta-D-glucan-4-glucanohydrolase, (1->3,1->4)-beta-glucanase isoenzyme EII, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, 1,3-1,4-beta-D-glucan glucanohydrolase, 1,3-1,4-beta-D-glucan-4-glucano hydrolase, 1,3-1,4-beta-D-glucanase, 1,3-1,4-beta-glucanase, 1,3;1,4-beta-glucan 4-glucanohydrolase, 1,3;1,4-beta-glucan endohydrolase, Af-EGL7, Afu6g01800, Beg1, beta-(1,3-1,4)-glucanase, beta-(1--> 3), (1--> 4)-D-glucan 4-glucanohydrolase, beta-1,3-1,4 glucanase, beta-1,3-1,4-D-glucanase, beta-1,3-1,4-glucanase, beta-1,3;1,4-glucanase, beta-1-3, 1-4 glucan 4-glucanohydrolase, beta-glucanase, BG1, Bg1314, Bga1, bgc, bgi, Bgl, bgl5-1, BglA, BglA13, BglA16, BglA51, BglBB, bglBC1, BGlc8H, BglM2, BglS, BglT, BglTO, Bglu16A, bifunctional xylanase/endoglucanase, BLB369, BLB369 endo-beta-1,3-1,4-glucanase, Blc8H, BP_Cel9A, Cel5F, CP7 beta-1,3-1,4-glucanase, CtGlu16A, Cthe_0211, CtLic16A, CtLic26A, E-LICHN, EG1, EGL, EII, endo-(1,3)(1,4)-beta-glucanase, endo-(1,3;1,4)-beta-glucanase, endo-beta-1,3-1,4 glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3;1,4-glucan-D-glycosyl hydrolase, endo-beta-glucanase, endoglucanase, endotype beta-1,3-1,4-glucanase, Fisuc_2961, Fsbeta-glucanase, FSU_0226, Gcs2, GH7 endo-1,4-beta-glucanase, GHF16 TFsbeta-glucanase, GHF17 barley 1,3-1,4-beta-D-glucanase, Glc16A, GLU-1, GLU-3, glu369, Gluc5_26A, GluIII, GluUS570, glycoside hydrolase family 9 endoglucanase, GyrA, H(A16-M), lam1, LamA, laminarinase, Lic16A, Lic8H, LicA, LicB, Lichenase, lichenase-2, LicKM, licM, LicMB, LicS, McLic1, mHG, Mixed linkage beta-glucanase, More, NFEg16A, PbBglu16A, PlicA, PtLic16A, Ra0505, RuCelA, TaGlu34, TC2, TC5, TF-glu, TF-glucanase, theme C glycoside hydrolase family 9 endo-beta-glucanase, TM1752, US8_01508, XynIII, XynZ, ZgLamA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.73 licheninase

pH Stability

pH Stability on EC 3.2.1.73 - licheninase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 2
purified enzyme, 37°C, 30 min, inactivation
730139
1 - 8
37°C, 1 h, more than 85% of maximum activity
707242
11
purified enzyme, 37°C, 30 min, loss of 50% activity
730139
2
1 h, wild-type 5% residual activity, mutant T113S 40% residual activity, mutant M44V/N53H 50% residual activity, mutant M44V/N53H 80% residual activity
738548
2.5 - 9
-
4°C or 20°C, stable for 20 h
171529
3
-
retains approximately 80% of original activity after treatment at pH 3.0 for 12 h
677749
3 - 10
3 - 11
purified recombinant His-tagged enzyme, 50°C, 30 min, completely stable
729409
3 - 8
completely stable up to pH 6.0, 40% activity remaining at pH 7.0, inactivation at pH 8.0
752108
3.4 - 9.8
-
4°C, stable for at least 24 h
171538
3.5 - 12
the enzyme is acid-, alkali- and salt-tolerant, the purified recombinant His-tagged enzyme retains more than 80% activity after incubation for 4 h
729476
3.5 - 7
3.5 - 8
purified recombinant enzyme, 30 min, over 80% activity remaining
750809
3.5 - 8.5
-
the enzyme remains stable between pH 3.5 and 8.5 after 30 min incubation
744957
3.5 - 9
3.6 - 10.6
-
-
681336
4
-
optimum condition for the beta-glucanase stability
681159
4 - 10
4 - 5
-
25°C, 72-96 h, stable
171531
4 - 6
-
the recombinant enzyme is stable between pH 4.0 and 6.0, having 85% of the original activity
677749
4 - 7
-
increase in stability from pH 4 to pH 7
657085
4 - 8
Thermomonospora sp.
-
-
678492
4 - 9
4.8 - 6
-
stable
171537
4.8 - 6.4
-
stable
171537
5 - 6
-
60°C, 10 min, stable
171533
5 - 7
-
10 min, stable
171536
5 - 7.5
purified native enzyme, retains 90% of its activity at 60°C for 30 min
729865
5.5 - 7.5
-
the enzyme retains about 60% activity at pH 5.5 and 90% at pH 7.5 after 10 min of incubation
746205
5.6 - 6
6
-
no enzyme activity is detected at pH higher than 6
678923
6 - 10
-
stable at
751414
6 - 9
8
-
retains approximately 80% of original activity after treatment at pH 8.0 for 12 h
677749