3.2.1.73: licheninase
This is an abbreviated version!
For detailed information about licheninase, go to the full flat file.
Word Map on EC 3.2.1.73
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3.2.1.73
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cellulases
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cellulolytic
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trichoderma
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knee
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reesei
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cellobiohydrolase
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exoglucanase
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thermocellum
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avicel
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carboxymethyl
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lignocellulosic
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cellulosic
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arthroscopic
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saccharification
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beta-glucosidase
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carboxymethylcellulose
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cellulose-binding
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straw
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microcrystalline
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glutenins
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cellulosome
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bagasse
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cellotriose
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lichenan
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xyloglucans
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cellulomonas
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cellotetraose
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xylanases
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3.2.1.4
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hemicellulases
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cmcase
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patellar
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endo-beta-1,4-glucanase
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patellofemoral
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succinogenes
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endoxylanase
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dockerins
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humicola
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fpase
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fibrobacter
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cellulose-degrading
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lysholm
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lignocellulolytic
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mannanase
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cellodextrins
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meniscal
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cellobiase
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thermobifida
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xylanolytic
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brewing
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synthesis
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endocellulase
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agriculture
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biofuel production
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degradation
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food industry
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analysis
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industry
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nutrition
- 3.2.1.73
- cellulases
-
cellulolytic
- trichoderma
- knee
- reesei
- cellobiohydrolase
- exoglucanase
- thermocellum
- avicel
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carboxymethyl
-
lignocellulosic
-
cellulosic
-
arthroscopic
-
saccharification
- beta-glucosidase
- carboxymethylcellulose
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cellulose-binding
- straw
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microcrystalline
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glutenins
- cellulosome
- bagasse
- cellotriose
- lichenan
- xyloglucans
- cellulomonas
- cellotetraose
- xylanases
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3.2.1.4
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hemicellulases
- cmcase
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patellar
- endo-beta-1,4-glucanase
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patellofemoral
- succinogenes
- endoxylanase
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dockerins
- humicola
-
fpase
- fibrobacter
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cellulose-degrading
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lysholm
-
lignocellulolytic
- mannanase
- cellodextrins
- meniscal
- cellobiase
- thermobifida
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xylanolytic
- brewing
- synthesis
- endocellulase
- agriculture
- biofuel production
- degradation
- food industry
- analysis
- industry
- nutrition
Reaction
Synonyms
(1,3)(1,4)-beta-D-glucan-4-glucanohydrolase, (1->3,1->4)-beta-glucanase isoenzyme EII, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, 1,3-1,4-beta-D-glucan glucanohydrolase, 1,3-1,4-beta-D-glucan-4-glucano hydrolase, 1,3-1,4-beta-D-glucanase, 1,3-1,4-beta-glucanase, 1,3;1,4-beta-glucan 4-glucanohydrolase, 1,3;1,4-beta-glucan endohydrolase, Af-EGL7, Afu6g01800, Beg1, beta-(1,3-1,4)-glucanase, beta-(1--> 3), (1--> 4)-D-glucan 4-glucanohydrolase, beta-1,3-1,4 glucanase, beta-1,3-1,4-D-glucanase, beta-1,3-1,4-glucanase, beta-1,3;1,4-glucanase, beta-1-3, 1-4 glucan 4-glucanohydrolase, beta-glucanase, BG1, Bg1314, Bga1, bgc, bgi, Bgl, bgl5-1, BglA, BglA13, BglA16, BglA51, BglBB, bglBC1, BGlc8H, BglM2, BglS, BglT, BglTO, Bglu16A, bifunctional xylanase/endoglucanase, BLB369, BLB369 endo-beta-1,3-1,4-glucanase, Blc8H, BP_Cel9A, Cel5F, CP7 beta-1,3-1,4-glucanase, CtGlu16A, Cthe_0211, CtLic16A, CtLic26A, E-LICHN, EG1, EGL, EII, endo-(1,3)(1,4)-beta-glucanase, endo-(1,3;1,4)-beta-glucanase, endo-beta-1,3-1,4 glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3;1,4-glucan-D-glycosyl hydrolase, endo-beta-glucanase, endoglucanase, endotype beta-1,3-1,4-glucanase, Fisuc_2961, Fsbeta-glucanase, FSU_0226, Gcs2, GH7 endo-1,4-beta-glucanase, GHF16 TFsbeta-glucanase, GHF17 barley 1,3-1,4-beta-D-glucanase, Glc16A, GLU-1, GLU-3, glu369, Gluc5_26A, GluIII, GluUS570, glycoside hydrolase family 9 endoglucanase, GyrA, H(A16-M), lam1, LamA, laminarinase, Lic16A, Lic8H, LicA, LicB, Lichenase, lichenase-2, LicKM, licM, LicMB, LicS, McLic1, mHG, Mixed linkage beta-glucanase, More, NFEg16A, PbBglu16A, PlicA, PtLic16A, Ra0505, RuCelA, TaGlu34, TC2, TC5, TF-glu, TF-glucanase, theme C glycoside hydrolase family 9 endo-beta-glucanase, TM1752, US8_01508, XynIII, XynZ, ZgLamA
ECTree
3.2.1.73 licheninaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 3.2.1.73 - licheninase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
100
20 - 40
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the remaining activity decreased to 20% at 20-40°C and is completely destroyed at 50-70°C
22
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11 days, complete inactivation of wild-type, 70% residual activity of mutant D70V
25 - 40
purified recombinant enzyme, pH 6.0, 70 min, completely stable
30 - 60
30 - 90
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temperature effects on mutant W203F and on W203F mutant bifunctional hybrid enzymes, the latter is more resistant to heat treatment than the parental TFsW203F, overview
40
40 - 50
-
purified isozymes EG1 and EG2, retaining over 90% activity after 1 h
40 - 55
45
50
50 - 60
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sharp decrease in activity up to 60°C, but very stable below 50°C
50 - 70
55
60
60 - 70
65
70
75
80
80 - 90
purified recombinant enzyme, 70% and 40% activity remaining after 2 h at 80°C and 90°C, respectively, half-lives of the enzyme are 3.4 h and 1.4 h at 80°C and 90°C, respectively
85
90
additional information
100
purified native enzyme, pH 5.5, 40% activity remaining after 30 min
100
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even when subjected to 100°C for 3 h, beta-glucanase activity does not show significant reduction
30 - 60
completely stable up to 50°C for 1 h and loss of 10% activity after 48 h at 50°C, 50% activity remaing at 55°C after 30 min and inactivation after 5 h at 55°C, rapid inactivation at 60°C
30 - 60
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99.1, 87.8, 56.6, and 34.2% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
30 - 60
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104.5, 101.2, 67.9, and 21.2% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
30 - 60
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102, 91.2, 60.7, and 4.3% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
30 - 60
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98, 74.3, 2.4, and 0% residual activity after 30 min preincubation at 30, 40, 50, and 60°C, respectively
40
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purified native enzyme, 75% activity remaining after 120 min
40 - 55
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the enzyme remains stable between 40 and 55°C after 30 min incubation
45
the wild-type maintains more than 85% of the original activity for 10 min
50
purified recombinant His-tagged enzyme, pH 6.0, 30 min, completely stable
50
purified recombinant His-tagged enzyme retaining more than 70% activity at up to 50°C for 2 h
50
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purified native enzyme, 60% activity remaining after 120 min
50 - 70
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after incubation at 50, 60, and 70°C for 30 min, the residual activity is 95, 60, and 0%, respectively
50 - 70
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after 2 h incubation at 50°C and 60°C, the residual activity remaines 100% and 50%, respectively, while the enzymatic activity is abolished after 3 min incubation at 70°C
50 - 70
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the purified native enzyme shows half-lives of 209, 130 and 69 min at 50, 60 and 70°C, respectively
55
purified recombinant His-tagged enzyme, half-life is about 50 min, 10% activity remaining after 2 h
60
purified recombinant enzyme, retains more than 90% activity after 2 h
60
2 h, more than 85% residual activity, both wild-type and mutant K142N/Q203L/N214D
60
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purified isozymes EG1 and EG2, half-lives are 55 min and 5 h, respectively
60
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pH 5.0-6.0, 10 min, stable. pH 6, in presence of 10 mM CaCl2 and serum albumin, stable for 120 min
60
half-life of wild-type 46.2 min, of mutant K48A 346 min, of mutant K48L 138 min, respectively
60
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the melting temperature is 50.8°C. The half-life of the enzyme at 60°C is 59 min. The enzyme retains more than 60% of its activity at up to 75°C and is inactivated at 80°C after 10 min of incubation
60
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4 min, 50% inactivation, wild-type, 11 min, 50% inactivation, mutant D70V
60
purified recombinant His-tagged enzyme, inactivation within 10 min, in presence of 5 mM CaCl2 the enzyme retains over 80% activity after 2 h
60
after 30 min and 1 h at 60°C, the enzyme retains about 60% and 35% of activity, respectively
60
purified native enzyme, no loss in activity at pH 5.5 for 30 min, half-life is 225 min
60 - 70
-
purified recombinant enzyme, the wild-type enzymes activity decline rapidly above 60°C, while the optimally-modified enzyme is more stable and shows activity up to 70°C
65
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hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase is stable for more than 1 h at pH 5.5. The 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase. Hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens irreversible thermoinactivated within 20-25 min
65
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the linear enzyme form loses 50% activity within 3 min, whereas the two circular variants have 6fold (LicA-C1) and 16fold (LicA-C2) increased half-life time of inactivation
65
purified recombinant His-tagged enzyme, pH 6.0, 30 min, loss of 50% activity
65
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hybrid beta-glucanase enzyme H1 which contains the 107 amino-terminal residues of mature Bacillus amyloliquefaciens beta-glucanase is stable for more than 1 h at pH 5.5. The 107 carboxyl-terminal amino acid residues of Bacillus beta-glucanase. Hybrid enzyme H2 which consists of the 105 amino-terminal residues from the Bacillus macerans enzyme and the carboxyl-terminal 107 amino acids from Bacillus amyloliquefaciens irreversible thermoinactivated within 20-25 min
65
purified recombinant His-tagged enzyme, in presence of 5 mM CaCl2 the enzyme retains 40% activity after 10 min, and 20% after 20 min, inactivation after 1 h
70
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purified recombinant enzyme, pH 7.0, 40% activity remaining after 6 h
70
purified recombinant enzyme, retains more 75% activity after 10 min
70
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pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity
70
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purified isozymes EG1 and EG2, half-lives are 10 min and 52 min, respectively
70
purified recombinant His-tagged enzyme, pH 6.0, 30 min, inactivation
70
half-life of wild-type 4.5 min, of mutant K48A 30.5 min, of mutant K48L 15.1 min, respectively
70
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pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity
70
purified recombinant enzyme, completely stable for 8 h, and loss of 20% activity after 22 h
75
-
purified recombinant wild-type enzyme, 10 min, 51% remaining activity in absence of Ca2+, 86% in presence of 1 mM Ca2+
80
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purified recombinant wild-type enzyme, 5 min, 54% remaining activity in absence of Ca2+, 73% in presence of 1 mM Ca2+
80
-
purified recombinant enzyme, pH 7.0, no activity remaining after 1 h
80
purified recombinant enzyme, retains more 44% activity after 10 min
80
purified recombinant enzyme, retains 54% activity at 80°C after incubation for 60 min
80
4 min, complete loss of activtiy for wild-type, 10 min, 60% residual activity for mutant T113S
80
purified native enzyme, pH 5.5, half-life is 53 min, 80% activity remaining after 30 min
85
purified native enzyme, pH 5.5, half-life is 27 min, inactivation after 120 min
90
-
the residual activity of mHG at 90°C for 10 min is 83.45% of its maximum activity
90
purified native enzyme, pH 5.5, 60% activity remaining after 30 min
additional information
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Ca2+ stabilizes the enzyme at higher temperatures, thermal stability of recombinant wild-type enzyme and mutants in presence and absence of Ca2+, overview
additional information
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particularly thermostable in presence of Ca2+
additional information
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Ca2+ stabilizes hybrid enzymes and parental enzymes against thermal inactivation
additional information
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addition of 1 mM EDTA causes the linear enzyme to denature at 59.8°C, which is lower compared to the melting temperature of 62.6°C in the presence of calcium. At 1 mM EDTA, the circular enzyme variant LicA-C1 shows a melting temperature of 62.8°C, at 1 mM CaCl2 the melting temperature is 66.4°C
additional information
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particularly thermostable in presence of Ca2+
additional information
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particularly thermostable in presence of Ca2+
additional information
-
thermal inactivation follow first-order kinetics, overview
additional information
-
particularly thermostable in presence of Ca2+
additional information
-
Ca2+ stabilizes hybrid enzymes and parental enzymes against thermal inactivation
additional information
-
particularly thermostable in presence of Ca2+
