3.2.1.73: licheninase
This is an abbreviated version!
For detailed information about licheninase, go to the full flat file.
Word Map on EC 3.2.1.73
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3.2.1.73
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cellulases
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cellulolytic
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trichoderma
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knee
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reesei
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cellobiohydrolase
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exoglucanase
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thermocellum
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avicel
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carboxymethyl
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lignocellulosic
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cellulosic
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arthroscopic
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saccharification
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beta-glucosidase
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carboxymethylcellulose
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cellulose-binding
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straw
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microcrystalline
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glutenins
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cellulosome
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bagasse
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cellotriose
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lichenan
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xyloglucans
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cellulomonas
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cellotetraose
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xylanases
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3.2.1.4
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hemicellulases
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cmcase
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patellar
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endo-beta-1,4-glucanase
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patellofemoral
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succinogenes
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endoxylanase
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dockerins
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humicola
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fpase
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fibrobacter
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cellulose-degrading
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lysholm
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lignocellulolytic
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mannanase
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cellodextrins
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meniscal
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cellobiase
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thermobifida
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xylanolytic
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brewing
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synthesis
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endocellulase
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agriculture
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biofuel production
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degradation
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food industry
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analysis
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industry
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nutrition
- 3.2.1.73
- cellulases
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cellulolytic
- trichoderma
- knee
- reesei
- cellobiohydrolase
- exoglucanase
- thermocellum
- avicel
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carboxymethyl
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lignocellulosic
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cellulosic
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arthroscopic
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saccharification
- beta-glucosidase
- carboxymethylcellulose
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cellulose-binding
- straw
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microcrystalline
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glutenins
- cellulosome
- bagasse
- cellotriose
- lichenan
- xyloglucans
- cellulomonas
- cellotetraose
- xylanases
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3.2.1.4
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hemicellulases
- cmcase
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patellar
- endo-beta-1,4-glucanase
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patellofemoral
- succinogenes
- endoxylanase
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dockerins
- humicola
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fpase
- fibrobacter
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cellulose-degrading
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lysholm
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lignocellulolytic
- mannanase
- cellodextrins
- meniscal
- cellobiase
- thermobifida
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xylanolytic
- brewing
- synthesis
- endocellulase
- agriculture
- biofuel production
- degradation
- food industry
- analysis
- industry
- nutrition
Reaction
Synonyms
(1,3)(1,4)-beta-D-glucan-4-glucanohydrolase, (1->3,1->4)-beta-glucanase isoenzyme EII, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, 1,3-1,4-beta-D-glucan glucanohydrolase, 1,3-1,4-beta-D-glucan-4-glucano hydrolase, 1,3-1,4-beta-D-glucanase, 1,3-1,4-beta-glucanase, 1,3;1,4-beta-glucan 4-glucanohydrolase, 1,3;1,4-beta-glucan endohydrolase, Af-EGL7, Afu6g01800, Beg1, beta-(1,3-1,4)-glucanase, beta-(1--> 3), (1--> 4)-D-glucan 4-glucanohydrolase, beta-1,3-1,4 glucanase, beta-1,3-1,4-D-glucanase, beta-1,3-1,4-glucanase, beta-1,3;1,4-glucanase, beta-1-3, 1-4 glucan 4-glucanohydrolase, beta-glucanase, BG1, Bg1314, Bga1, bgc, bgi, Bgl, bgl5-1, BglA, BglA13, BglA16, BglA51, BglBB, bglBC1, BGlc8H, BglM2, BglS, BglT, BglTO, Bglu16A, bifunctional xylanase/endoglucanase, BLB369, BLB369 endo-beta-1,3-1,4-glucanase, Blc8H, BP_Cel9A, Cel5F, CP7 beta-1,3-1,4-glucanase, CtGlu16A, Cthe_0211, CtLic16A, CtLic26A, E-LICHN, EG1, EGL, EII, endo-(1,3)(1,4)-beta-glucanase, endo-(1,3;1,4)-beta-glucanase, endo-beta-1,3-1,4 glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3;1,4-glucan-D-glycosyl hydrolase, endo-beta-glucanase, endoglucanase, endotype beta-1,3-1,4-glucanase, Fisuc_2961, Fsbeta-glucanase, FSU_0226, Gcs2, GH7 endo-1,4-beta-glucanase, GHF16 TFsbeta-glucanase, GHF17 barley 1,3-1,4-beta-D-glucanase, Glc16A, GLU-1, GLU-3, glu369, Gluc5_26A, GluIII, GluUS570, glycoside hydrolase family 9 endoglucanase, GyrA, H(A16-M), lam1, LamA, laminarinase, Lic16A, Lic8H, LicA, LicB, Lichenase, lichenase-2, LicKM, licM, LicMB, LicS, McLic1, mHG, Mixed linkage beta-glucanase, More, NFEg16A, PbBglu16A, PlicA, PtLic16A, Ra0505, RuCelA, TaGlu34, TC2, TC5, TF-glu, TF-glucanase, theme C glycoside hydrolase family 9 endo-beta-glucanase, TM1752, US8_01508, XynIII, XynZ, ZgLamA
ECTree
3.2.1.73 licheninaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.1.73 - licheninase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.7
2,4-dinitrophenyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
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pH 7.2, 30°C
0.7
3,4-dinitrophenyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
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pH 7.2, 30°C
1
4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
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pH 7.2, 30°C
0.5
4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
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pH 7.2, 30°C
0.6
4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
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pH 7.2, 30°C
0.34
beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride
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pH 7.0, 35°C
0.15
beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride
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pH 7.0, 35°C
5.3
Glcbeta3Glcbeta-methylumbelliferone
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pH 7.0, 37°C, 50 mM sodium phosphate buffer
0.37
4-methylumbelliferyl 3-O-beta-cellobiosyl-beta-D-glucoside
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pH 6.9, 30°C
0.5
4-methylumbelliferyl 3-O-beta-cellobiosyl-beta-D-glucoside
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pH 7.2, 30°C
0.6
4-methylumbelliferyl 3-O-beta-cellobiosyl-beta-D-glucoside
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pH 7.8, 30°C
0.271
azo blue barley beta-glucan
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mutant E46P/S43E/H205P/S40E, pH 6.5, 40°C
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0.278
azo blue barley beta-glucan
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mutant N31C/T187C/P102C/N125C, pH 6.5, 40°C
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0.27
azo-barley glucan
mutant K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, pH 6.5, 40°C
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0.2775
Barley beta-glucan
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mutant N31C/T187C/P102C/N125C, at pH 6.5 and 40°C
additional information
barley beta-D-glucan
Km value of wild-type 13.5 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 13.3 mg/ml, respectively, pH 5.0, 60°C
additional information
barley beta-D-glucan
Km value of wild-type 19.7 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 16.3 mg/ml, respectively, pH 5.0, 40°C
additional information
Barley beta-glucan
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4.36 mg/ml with substrate barley beta-glucan
additional information
Barley beta-glucan
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wild-type, 2.1 mg/ml, mutant K48A 1.6 mg/ml, mutant K48L 1.4 mg/ml, respectively, pH 6.0, 50°C
additional information
Barley beta-glucan
Km value 2.23 mg/ml, pH 6.0, 55°C
additional information
Barley beta-glucan
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Km value 2.23 mg/ml, pH 6.0, 55°C
additional information
Barley beta-glucan
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Km value 7.42 mg/ml, pH 6.5, 60°C
additional information
Barley beta-glucan
Km value of wild-type 2.1 mg/ml, of mutant K48A 1.6 mg/ml, of mutant K48L 1.4 mg/ml, respectively, pH 6.0, 50°C
additional information
Barley beta-glucan
Km: 0.67 mg/ml, pH 6.6, 70°C
additional information
carboxymethylcellulose
Km-value: 2.71 g/l at 50°C, pH5.0
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additional information
lichenan
Km value 1.82 mg/ml, pH 6.0, 55°C
additional information
lichenan
Km value 4 mg/ml for wild-type, 3.21 mg/ml for mutant K142N/Q203L/N214D
additional information
lichenin
Km value of wild-type 0.91 mg/ml, mutant T113S 0.69 mg/ml, mutant M44V/N53H 0.67 mg/ml, mutant N157D 0.70 mg/ml, respectively, pH 6.4, 40°C
additional information
additional information
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wild-type and mutant enzymes
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additional information
additional information
KM value for native enzyme, substrate beta-D-glucan, is 1.5 mg/ml, for fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate beta-D-glucan, is 1.2 mg/ml
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additional information
additional information
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KM value for native enzyme, substrate beta-D-glucan, is 1.5 mg/ml, for fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate beta-D-glucan, is 1.2 mg/ml
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additional information
additional information
KM value for native enzyme, substrate beta-D-glucan, is 2.7 mg/ml, for fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate beta-D-glucan, 1.2 mg/ml
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additional information
additional information
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KM value for native enzyme, substrate beta-D-glucan, is 2.7 mg/ml, for fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, substrate beta-D-glucan, 1.2 mg/ml
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additional information
additional information
KM-value for wild-type, substrate lichenan, 2.88 mg/ml
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additional information
additional information
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KM-value for wild-type, substrate lichenan, 2.88 mg/ml
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additional information
additional information
KM079629
0.45 mg/ml with lichenan at pH 6.0, 50°C
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additional information
additional information
0.69 mg/ml with barley beta-glucan, 1.11 mg/ml with oat beta-glucan, and 0.63 with lichenan, pH 10.0, 55°C
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additional information
additional information
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0.69 mg/ml with barley beta-glucan, 1.11 mg/ml with oat beta-glucan, and 0.63 with lichenan, pH 10.0, 55°C
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additional information
additional information
0.86 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.86 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.86 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.86 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
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0.86 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.90 mg/ml barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.90 mg/ml barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.90 mg/ml barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.90 mg/ml barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
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0.90 mg/ml barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.94 mg/ml with barley beta-glucan, at recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.94 mg/ml with barley beta-glucan, at recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.94 mg/ml with barley beta-glucan, at recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
0.94 mg/ml with barley beta-glucan, at recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
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0.94 mg/ml with barley beta-glucan, at recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
1.19 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
1.19 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
1.19 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
1.19 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
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1.19 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C
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additional information
additional information
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1.65 mg/ml with beta-1,3-1,4-glucan, at pH 6.0, 70°C
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additional information
additional information
10.0 mg/ml with barley beta-glucan, pH 6.0, 40°C, recombinant enzyme
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additional information
additional information
2.0 mg/ml with barley beta-glucan and 1.4 mg/ml with lichenan at pH 5.5, 60°C
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additional information
additional information
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2.1 mg/ml for isozyme EG1 and 1.8 mg/ml for isozyme EG2 with lichenan at pH 5.0, 50°C
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additional information
additional information
2.25 mg/ml for wild-type enzyme, 2.44 mg/ml for mutant V18Y, 3.67 mg/ml for mutant W203Y, and 3.29 mg/ml for mutant V18Y/W203Y, with lichenan, pH 5.0, 50°C, calculated as reducing sugar released from substrate
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additional information
additional information
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3.69 mg/ml for the wild-type enzyme and 3.30 mg/ml for mutant D56G/D221G/C263S, with barley beta-glucan at pH 5.0, 70°C
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additional information
additional information
3.7 mg/ml at pH 6.2, 55°C with barley beta-glucan, recombinant enzyme
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additional information
additional information
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5.3 mg/ml for mutant TFsW203F with lichenan, pH 6.0-7.0, 45°C. Kinetic properties of parental TFsW203F and mutant hybrid glucanases, overview
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additional information
additional information
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5.96 mg/ml with lichenan at pH 5.5, 50°C
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additional information
additional information
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7.9 mg/ml for recombinant wild-type enzyme, 7.2 mg/ml for recombinant optimally-modified enzyme, with AZO blue beta-glucan at pH 6.5, 40°C
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additional information
additional information
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Michaelis-Menten kinetics, 0.62 mg/ml with barley beta-glucan at pH 5.0, 60°C,and 0.38 mg/ml with lichenan at pH 5.0, 60°C
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additional information
additional information
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0.62 mg/ml with barley beta-glucan, pH 5.0, 60°C
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additional information
additional information
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0.67 mg/ml with barley beta-glucan, pH 4.0, 75°C
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additional information
additional information
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0.69 mg/ml with barley beta-glucan, 1.11 mg/ml with oat beta-glucan, and 0.63 mg/ml with lichenan, pH 5.0, 60°C
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additional information
additional information
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22.39 mg/ml with barley beta-glucan, pH 4.0-5.0, 50-60°C
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additional information
additional information
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5.957 mg/ml with lichenan, pH 3.0, 50-60°C
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additional information
additional information
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Km and Vmax values of the purified TaGlu34 for barley beta-D-glucan and oat beta-glucan are determined to be 2.09 mg/ml and 13.18 mmol/min/mg, and 1.36 mg/ml and 0.0165 mmol/min/mg, respectively
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additional information
additional information
Km for carboxymethyl cellulose is 209.7 mg/ml and for barley beta-glucan 113.9 mg/ml in presence of 5 mM Mn2+
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additional information
additional information
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Km for carboxymethyl cellulose is 209.7 mg/ml and for barley beta-glucan 113.9 mg/ml in presence of 5 mM Mn2+
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additional information
additional information
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Km is 13.38 mg/ml and Vmax is 0.1429 mM/min/mg with lichenan, pH 4.8, 80°C
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additional information
additional information
Km is 2.4 mg/ml and Vmax is 9.981 mmol/min/mg, with barley beta-glucan, pH 7.0, 70°C
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