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3.2.1.73: licheninase

This is an abbreviated version!
For detailed information about licheninase, go to the full flat file.

Word Map on EC 3.2.1.73

Reaction

beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranose
+ 4 H2O = 5 beta-D-glucopyranose

Synonyms

(1,3)(1,4)-beta-D-glucan-4-glucanohydrolase, (1->3,1->4)-beta-glucanase isoenzyme EII, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, 1,3-1,4-beta-D-glucan glucanohydrolase, 1,3-1,4-beta-D-glucan-4-glucano hydrolase, 1,3-1,4-beta-D-glucanase, 1,3-1,4-beta-glucanase, 1,3;1,4-beta-glucan 4-glucanohydrolase, 1,3;1,4-beta-glucan endohydrolase, Af-EGL7, Afu6g01800, Beg1, beta-(1,3-1,4)-glucanase, beta-(1--> 3), (1--> 4)-D-glucan 4-glucanohydrolase, beta-1,3-1,4 glucanase, beta-1,3-1,4-D-glucanase, beta-1,3-1,4-glucanase, beta-1,3;1,4-glucanase, beta-1-3, 1-4 glucan 4-glucanohydrolase, beta-glucanase, BG1, Bg1314, Bga1, bgc, bgi, Bgl, bgl5-1, BglA, BglA13, BglA16, BglA51, BglBB, bglBC1, BGlc8H, BglM2, BglS, BglT, BglTO, Bglu16A, bifunctional xylanase/endoglucanase, BLB369, BLB369 endo-beta-1,3-1,4-glucanase, Blc8H, BP_Cel9A, Cel5F, CP7 beta-1,3-1,4-glucanase, CtGlu16A, Cthe_0211, CtLic16A, CtLic26A, E-LICHN, EG1, EGL, EII, endo-(1,3)(1,4)-beta-glucanase, endo-(1,3;1,4)-beta-glucanase, endo-beta-1,3-1,4 glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3;1,4-glucan-D-glycosyl hydrolase, endo-beta-glucanase, endoglucanase, endotype beta-1,3-1,4-glucanase, Fisuc_2961, Fsbeta-glucanase, FSU_0226, Gcs2, GH7 endo-1,4-beta-glucanase, GHF16 TFsbeta-glucanase, GHF17 barley 1,3-1,4-beta-D-glucanase, Glc16A, GLU-1, GLU-3, glu369, Gluc5_26A, GluIII, GluUS570, glycoside hydrolase family 9 endoglucanase, GyrA, H(A16-M), lam1, LamA, laminarinase, Lic16A, Lic8H, LicA, LicB, Lichenase, lichenase-2, LicKM, licM, LicMB, LicS, McLic1, mHG, Mixed linkage beta-glucanase, More, NFEg16A, PbBglu16A, PlicA, PtLic16A, Ra0505, RuCelA, TaGlu34, TC2, TC5, TF-glu, TF-glucanase, theme C glycoside hydrolase family 9 endo-beta-glucanase, TM1752, US8_01508, XynIII, XynZ, ZgLamA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.73 licheninase

Inhibitors

Inhibitors on EC 3.2.1.73 - licheninase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3,4)-epoxybutyl beta-D-cellobioside
-
identification of Glu105 at the active site
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
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2,4,6-Trinitrobenzenesulfonic acid
Thermomonospora sp.
-
-
2,4-dinitrophenyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
-
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2-mercaptoethanol
3,4-dinitrophenyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
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4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
-
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4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
-
-
4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc
-
-
5,5'-dithiobis 2-nitrobenzoic acid
Thermomonospora sp.
-
-
Al3+
35% inhibition at 10 mM
beta-mercaptoethanol
-
inhibits the enzyme activity at 4 mM by 82.7%
CaCl2
-
10 mM, 33% inhibition
dithiothreitol
-
1 mM, 21% inhibition
EGTA
10 mM, 24% inhibition
epoxyalkyl cellobiosides
-
Glc-beta-1,3-isofagomine
-
-
glucono-delta-lactone
-
-
H2O2
-
at 0.5% w/v hydrogen peroxide, isozymes EG1 and EG2 retain 82.8% and 90.28% of their activities, respectively. At 1% w/v hydrogen peroxide, isozyme EG2 retains 60% activity, while isozyme EG1 is completely inhibited
hexadecyltrimethylammonium bromide
-
1 mM, no residual activity
imidazole
competitive
N-bromosuccinimide
-
1 mM, no residual activity
Na+
-
inhibits the enzyme activity at 4 mM by 47.3%; inhibits the enzyme activity at 4 mM by 80.3%
NaCl
-
30%, halflife of wild-type 230 h, mutant K48A 694 h, mutant K48L 693 h, respectively
Ni2+
-
inhibits the enzyme activity at 4 mM by 11.5%
o-phthaldehyde
Thermomonospora sp.
-
-
Sodium dodecyl sulfate
strong inhibition
sodium perborate
-
isozymes EG1 and EG2 retain 58% and 66.8% of their activities after 1 h of incubation at 40°C in the presence of 0.2% w/v sodium perborate, respectively
Sr2+
-
inhibits the enzyme activity at 4 mM by 49%
Urea
-
14.5% residual activity at 7 mM
additional information
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