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3.2.1.63: 1,2-alpha-L-fucosidase

This is an abbreviated version!
For detailed information about 1,2-alpha-L-fucosidase, go to the full flat file.

Word Map on EC 3.2.1.63

Reaction

(Glcalpha(1-4))n
+
H2O
=
(Glcalpha(1-4))n-4
 +
(Glcalpha(1-4))4

Synonyms

1,2-alpha-L-fucosidase, Afc3, AfcA, AfcA protein, alpha(1,2) fucosidase, alpha-(1--> 2)-L-fucosidase, alpha-fucosidase, alpha-L-fucosidase, alpha-L-fucosidase II, alpha1,2-FUC, alpha1,2-fucosidase, bfo_2737, FgFCO1, Fuc1, FucNaFLD, fucosidase, 1,2-alpha-L-, More

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.63 1,2-alpha-L-fucosidase

Cloned

Cloned on EC 3.2.1.63 - 1,2-alpha-L-fucosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression of 1,2-alpha-L-fucosidase in Escherichia coli, expression of the Fuc domain (responsible for fucosidase activity) as a hexahistidine-tagged protein in Escherichia coli
gene afcA, expression in Escherichia coli and in enzyme-deficient Bifidobacterium longum strain 105-A which renders the transformant able to utilize 2'-fucosyllactose as the sole carbon source
-
overexpressed in Escherichia coli as 6×His-tagged protein
-
overexpression in Escherichia coli, selenomethionine-substituted protein
-
sequence comparisons, recombinant enzyme expression of the enzyme including its native signal peptide from pPICZAalpha without the C-terminal Myc/His6 tags in Pichia pastoris, expression of the His6-tagged crystallin domain, residues 501-585, of the enzyme fused to maltose-binding protein at the N-terminus in Escherichia coli
wild-type and mutant derivatives of the catalytic domain of AfcA (Fuc domain)
-