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3.2.1.6: endo-1,3(4)-beta-glucanase

This is an abbreviated version!
For detailed information about endo-1,3(4)-beta-glucanase, go to the full flat file.

Word Map on EC 3.2.1.6

Reaction

(Glcbeta(1-3))n
+
H2O
=
Laminaripentaose
 +
(Glcbeta(1-3))n-5

Synonyms

1,3-(4)-beta-glucanase (endoglucanase), 1,3-1,4-beta-glucanase EII (barley), 1,3-beta-D-glucanase, AFUA_1G05290, AFUA_2G14360, AFUA_4G13360, AFUA_5G02280, Agl9A, AkLam36, barley-beta-D-glucanase, beta-1,3-1,4 glucanase, beta-1,3-1,4-glucanase, beta-1,3-glucanase, beta-1,3:1,4 glucanase, beta-D-(1-3)-glucanase, beta-glucanase, bglBC1, CBM-CD, CC1G_01665, Cel8A, CelL, chitosanase, CMCase, CtLic26A, enda-beta-(1-3),(1-4)-glucanase, endo beta-1,3-glucanase IV, endo-1,3(4)-beta-D-glucanase, endo-1,3(4)-beta-glucanase, endo-1,3-1,4-beta-D-glucanase, endo-1,3-beta-D-glucanase, endo-1,3-beta-glucanase, endo-1,4-beta-glucanase, endo-beta-(1-3)-D-glucanase, endo-beta-1,3(4)-glucanase, endo-beta-1,3-1,4-D-glucanase, endo-beta-1,3-1,4-glucanase, endo-beta-1,3-glucanase, endo-beta-1,4-glucanase, eng1, ENG16A, Eng2, Eng3, Eng4, Eng5, Engl1, Fsbeta-glucanase, GluA, H(A16-M), HdLam33, LAM, Lam16A, LamA, lamarinase, laminaranase, laminarinase, laminarinase 16A, laminarinase II, laminarinase Lam16A, LamR, LIC 1, Lic16A, Lic8H, Lichenase, More, nonlytic endo-beta-1,3-glucanase I, SsGlc, TcGlu2, TeEgl5A, Xyn10B

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.6 endo-1,3(4)-beta-glucanase

Temperature Stability

Temperature Stability on EC 3.2.1.6 - endo-1,3(4)-beta-glucanase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
15 min, 15% residual activity
20 - 60
-
at 20°C 8% loss of activity, loss of 80% of activity after heating at 60°C for 3 min
30 - 60
-
stable at 30°C, 25% of original activity lost after incubation at 40°C for 1 h, complete denaturation within 10 min at 60°C
30 - 75
30 min, pH 7.5, 20 mM Tris-HCl, 35-45% residual activtiy
37
-
with barley beta-glucan as substrate, 26% of activity after 30 min, no activity after 180 min
42
-
50% inactivation
50 - 60
-
stable for 20 min at 50°C, at 60°C loss of 75% of maximal activity
50 - 70
71
-
Tm-value for wild-type enzyme is 71.4°C
95 - 100
-
incubation at 100°C: 82% of initial activity at 95°C, 57% at 100°C
additional information