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2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucose
18% of the activity with 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
2.7% of the activity with 4-nitrophenyl alpha-D-glucopyranoside
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
3.9% of the activity with 4-nitrophenyl alpha-D-glucopyranoside
-
?
4-nitrophenyl beta-D-cellobiopyranoside + H2O
4-nitrophenol + D-cellobiose
3% of the activity with 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
9% of the activity with 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
4-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosaminide
-
-
-
-
?
amygdalin + H2O
D-glucose + ?
63% of the activity with laminarin
-
-
?
arbutin + H2O
D-glucose + ?
barley beta-(1->3)-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
D-glucose + ?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucoside + H2O
?
-
-
-
-
?
beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
-
-
-
-
?
carboxymethylpachyman + H2O
D-glucose
-
-
-
?
cell wall of Rhizoctania solani + H2O
?
-
-
-
?
cellobiose + H2O
2 D-glucose
cellobiose + H2O
?
-
3% of activity compared to laminaran
-
-
?
cellodextrin + H2O
D-glucose
-
-
-
?
cellotetraose + H2O
D-glucose + ?
cellotriose + H2O
?
-
-
-
-
?
cellotriose + H2O
D-glucose + ?
-
67% of the activity with laminaribiose
-
?
CM-cellulose + H2O
D-glucose
-
very weak hydrolysis
-
?
CM-pachyman + H2O
D-glucose
curdlan + H2O
D-glucose + ?
14% of the activity with laminarin from Laminaria digitata
-
-
?
esculin + H2O
D-glucose + 4-hydroxyphenol
gentiobiose + H2O
?
-
21% of activity compared to laminaran
-
-
?
gentiobiose + H2O
D-glucose
gentobiose + H2O
2 D-glucose
glucan + H2O
D-glucose + gentiobiose
laminaran + H2O
?
-
-
-
-
?
laminaran + H2O
D-glucose + ?
-
at pH 5.0, strictly specific for laminaran and laminarioligosaccharides, with a strict exo mode of action, with glucose being the sole reaction product even at the initial stages of hydrolysis
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
laminaribiose
6-O-glucosyl-laminaribiose
-
transglycosylation
-
-
?
laminaribiose + H2O
2 D-glucose
laminaribiose + H2O
D-glucose
laminarihexaose + 4 H2O
4 D-glucose + laminaribiose
laminarihexaose + 5 H2O
6 D-glucose
laminarihexaose + H2O
D-glucose + ?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
laminarin + H2O
alpha-D-glucose + ?
-
-
-
-
?
laminarin + H2O
beta-D-glucose + gentiobiose
lowest activity
-
-
?
laminarin + H2O
beta-D-glucose + laminaribiose
-
61% beta-D-glucose + 39% laminaribiose
-
-
?
laminarin + H2O
D-glucose + ?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
laminaripentaose + 3 H2O
3 D-glucose + laminaribiose
laminaripentaose + 4 H2O
4 D-glucose
-
-
-
?
laminaripentaose + H2O
D-glucose + ?
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
laminaritetraose + 2 H2O
2 D-glucose + laminaribiose
laminaritetraose + 3 H2O
4 D-glucose
laminaritetraose + H2O
D-glucose + ?
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
laminaritriose + 2 H2O
3 D-glucose
laminaritriose + H2O
D-glucose + ?
laminaritriose + H2O
D-glucose + laminaribiose
linear alpha-1,5-L-arabinan + H2O
?
luteose + H2O
D-glucose
-
-
-
?
maltose + H2O
2 D-glucose
-
-
-
-
?
N,N'-diacetyl-chitobiose + H2O
?
-
-
-
-
?
N-acetyl-beta-D-galactosaminide + H2O
?
-
-
-
-
?
N-acetyl-beta-D-glucosaminide + H2O
?
-
-
-
-
?
neutral glucan + H2O
D-glucose + laminaribiose
-
from Achlya
-
?
nigeran + H2O
?
-
-
-
-
?
o-nitrophenyl-beta-D-glucopyranoside + H2O
o-nitrophenol + D-glucose
-
-
-
?
oligoglucosides from pachyman + H2O
D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
22% of activity compared to laminaran
-
-
?
pachyman + H2O
D-glucose + ?
-
-
-
-
?
paramylon + H2O
D-glucose
-
-
-
?
pendulan + H2O
D-glucose
-
-
-
?
periodate-oxidized and reduced laminarin
glycerol + glycoaldehyde + D-glucose
phosphoglucan + H2O
?
-
-
-
-
?
polydatin + H2O
D-glucose + 3,4',5-trihydroxystilbene
-
-
-
?
pullulan + H2O
?
-
-
-
-
?
purified cell wall from Rhizoctonia solani + H2O
?
-
25% of activity compared to laminarin
-
-
?
pustulan + H2O
D-glucose + ?
-
-
-
-
?
salicin + H2O
D-glucose + 2-hydroxymethylphenol
-
-
-
?
schizophyllan + H2O
D-glucose + gentiobiose
scleroglucan + H2O
?
-
-
-
-
?
sclerotan + H2O
D-glucose + gentiobiose
sophorose + H2O
2 beta-D-glucose
-
-
-
-
?
sugar beet arabinan + H2O
?
wheat flour arabinoxylan + H2O
?
zymosan A + H2O
D-glucose + ?
additional information
?
-
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
-
-
-
?
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
-
-
-
-
?
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
-
-
-
?
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
31% of the activity with 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
31% of the activity with 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
13% of the activity with 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
4-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
-
-
?
amygdalin + H2O
?
-
82.2% of the activity with laminarin
-
-
?
amygdalin + H2O
?
-
82.2% of the activity with laminarin
-
-
?
arbutin + H2O
D-glucose + ?
-
-
-
?
arbutin + H2O
D-glucose + ?
-
-
-
?
barley beta-glucan + H2O
D-glucose + ?
-
7.4% of the activity with laminarin
-
-
?
barley beta-glucan + H2O
D-glucose + ?
-
7.4% of the activity with laminarin
-
-
?
beta-1,3-glucan + H2O
?
-
-
-
?
beta-1,3-glucan + H2O
?
-
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
-
?
cellobiose + H2O
2 D-glucose
-
62% of the activity with laminaribiose
-
?
cellobiose + H2O
2 D-glucose
-
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
?
cellotetraose + H2O
D-glucose + ?
-
-
-
-
?
cellotetraose + H2O
D-glucose + ?
-
64% of the activity with laminaribiose
-
?
cellotetraose + H2O
D-glucose + ?
-
-
-
-
?
CM-pachyman + H2O
D-glucose
-
-
-
?
CM-pachyman + H2O
D-glucose
-
very weak hydrolysis
-
?
curdlan + H2O
?
-
-
-
-
?
curdlan + H2O
?
-
-
-
-
?
curdlan + H2O
?
-
-
-
-
?
curdlan + H2O
?
-
-
-
-
?
curdlan + H2O
?
-
-
-
-
?
curdlan + H2O
?
-
-
-
-
?
esculin + H2O
?
-
24.2% of the activity with laminarin
-
-
?
esculin + H2O
?
-
24.2% of the activity with laminarin
-
-
?
esculin + H2O
D-glucose + 4-hydroxyphenol
-
-
-
?
esculin + H2O
D-glucose + 4-hydroxyphenol
-
-
-
?
gentiobiose + H2O
D-glucose
-
-
-
-
?
gentiobiose + H2O
D-glucose
-
-
-
?
gentiobiose + H2O
D-glucose
-
-
-
-
?
gentiobiose + H2O
D-glucose
-
-
-
-
?
gentiobiose + H2O
D-glucose
-
-
-
-
?
gentobiose + H2O
2 D-glucose
-
52% of the activity with laminaribiose
-
?
gentobiose + H2O
2 D-glucose
-
-
-
?
gentobiose + H2O
2 D-glucose
-
-
-
?
glucan + H2O
D-glucose + gentiobiose
-
Achlya neutral glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
-
Acremonium persicinum extracellular glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
-
yeast glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
-
Claviceps glucan
and laminaribiose
?
glucan + H2O
D-glucose + gentiobiose
-
Sclerotium rolfsii glucan
-
?
glucan + H2O
D-glucose + gentiobiose
-
yeast glucan
and laminaribiose
?
glucan + H2O
D-glucose + gentiobiose
-
Ganoderma applanatum glucan
-
?
glucan + H2O
D-glucose + gentiobiose
-
Claviceps glucan
and laminaribiose
?
glucan + H2O
D-glucose + gentiobiose
-
yeast glucan
and laminaribiose
?
glucan + H2O
D-glucose + gentiobiose
-
Sclerotium rolfsii glucan
-
?
glucan + H2O
D-glucose + gentiobiose
-
Ganoderma applanatum glucan
-
?
glucan + H2O
D-glucose + gentiobiose
-
barley beta-glucan
-
?
glucan + H2O
D-glucose + gentiobiose
-
Claviceps glucan
-
?
glucan + H2O
D-glucose + gentiobiose
-
yeast glucan
-
?
glucan + H2O
D-glucose + gentiobiose
-
oat beta-glucan
-
?
glucan + H2O
D-glucose + gentiobiose
Fungi imperfecti
-
yeast glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
-
barley beta-glucan
D-glucose only
?
glucan + H2O
D-glucose + gentiobiose
-
yeast glucan
D-glucose only
?
glucan + H2O
D-glucose + gentiobiose
-
oat beta-glucan
D-glucose only
?
glucan + H2O
D-glucose + gentiobiose
-
barley beta-glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
-
extracted and commerical barley beta-glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
-
Avena caryopsis glucan
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
-
from Eisenia bicyclis
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
-
from Eisenia bicyclis
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
-
-
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
-
-
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
-
-
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
-
-
almost all D-glucose
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
67% of the activity with laminaribiose
-
-
?
laminaribiose + H2O
2 D-glucose
-
-
-
-
?
laminaribiose + H2O
2 D-glucose
-
-
complete hydrolysis within 2 h to yield glucose
-
?
laminaribiose + H2O
2 D-glucose
-
-
complete hydrolysis within 2 h to yield glucose
-
?
laminaribiose + H2O
2 D-glucose
best substrate
the enzyme additioinally has transglycosylation activity, producing laminaritriose from laminaribiose
-
?
laminaribiose + H2O
2 D-glucose
best substrate
the enzyme additioinally has transglycosylation activity, producing laminaritriose from laminaribiose
-
?
laminaribiose + H2O
2 D-glucose
-
-
-
?
laminaribiose + H2O
?
-
-
-
-
?
laminaribiose + H2O
?
-
376% of activity compared to laminaran
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
-
?
laminariheptaose + H2O
?
-
-
-
-
?
laminariheptaose + H2O
?
-
-
-
-
?
laminarihexaose + 4 H2O
4 D-glucose + laminaribiose
-
-
-
?
laminarihexaose + 4 H2O
4 D-glucose + laminaribiose
-
-
-
?
laminarihexaose + 5 H2O
6 D-glucose
-
-
-
?
laminarihexaose + 5 H2O
6 D-glucose
-
-
-
?
laminarihexaose + H2O
?
-
-
-
?
laminarihexaose + H2O
?
highest activity
-
-
?
laminarihexaose + H2O
?
highest activity
-
-
?
laminarihexaose + H2O
D-glucose + ?
Thermochaetoides thermophila
-
-
-
?
laminarihexaose + H2O
D-glucose + ?
Thermochaetoides thermophila DSM 1495
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
-
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
Fungi imperfecti
-
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
-
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
-
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
AkLam33 exhibits exolytic beta-1,3-hydrolytic activity releasing D-glucose, it is highly active with high activity toward smaller substrates such as laminaritetraose and laminaritriose, and hydrolyzes highly branched beta-1,3-glucans such as Eisenia bicyclis laminarin, but it is also active toward beta-1,3-glucans with a few beta-1,6-linked glucose branches such as Laminaria digitata laminarin
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
exo-beta-1,3-glucanases hydrolyze laminarin by sequentially cleaving glucose residues from the non-reducing end of polymers or oligomers
-
-
?
laminarin + H2O
?
active site glutamate residue
-
-
?
laminarin + H2O
?
the purified native enzyme can convert laminarin into monosaccharides and disaccharides, but has no killer toxin activity
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
high activity in strain KH10
-
-
?
laminarin + H2O
?
-
from Laminaria digitata or Eisenia bicyclis
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
from Laminarium digitatum
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
?
laminarin + H2O
D-glucose + ?
-
most favorable substrate
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + ?
activity with laminaran from Eisnia bicyclic is 4% of the activity with laminarin from Laminaria digitata
-
-
?
laminarin + H2O
D-glucose + ?
Thermochaetoides thermophila
-
-
-
-
?
laminarin + H2O
D-glucose + ?
Thermochaetoides thermophila
-
-
-
?
laminarin + H2O
D-glucose + ?
Thermochaetoides thermophila CT2
-
-
-
-
?
laminarin + H2O
D-glucose + ?
Thermochaetoides thermophila DSM 1495
-
-
-
?
laminarin + H2O
D-glucose + ?
-
glucose is the sole hydrolysis product
-
?
laminarin + H2O
D-glucose + ?
-
glucose is the sole hydrolysis product
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
oxidized laminarin, reduced laminarin
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
Fungi imperfecti
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
ir
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminaripentaose + 3 H2O
3 D-glucose + laminaribiose
-
-
-
?
laminaripentaose + 3 H2O
3 D-glucose + laminaribiose
-
-
-
?
laminaripentaose + H2O
?
-
-
-
-
?
laminaripentaose + H2O
?
-
-
-
?
laminaripentaose + H2O
?
-
-
-
?
laminaripentaose + H2O
?
-
-
-
?
laminaripentaose + H2O
?
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
-
different combinations and amount of these products, depending on organism
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
Fungi imperfecti
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
-
different combinations and amount of these products, depending on organism
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
-
different combinations and amount of these products, depending on organism
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
118% of the activity with laminaran
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
-
-
-
?
laminaritetraose + 2 H2O
2 D-glucose + laminaribiose
-
-
-
?
laminaritetraose + 2 H2O
2 D-glucose + laminaribiose
-
-
-
?
laminaritetraose + 3 H2O
4 D-glucose
-
-
-
?
laminaritetraose + 3 H2O
4 D-glucose
-
-
-
?
laminaritetraose + H2O
?
-
-
-
-
?
laminaritetraose + H2O
?
-
preferred substrate
-
-
?
laminaritetraose + H2O
?
-
-
-
?
laminaritetraose + H2O
?
-
-
-
?
laminaritetraose + H2O
?
-
-
-
?
laminaritetraose + H2O
?
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
different combinations and amount of these products, depending on organism
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
Fungi imperfecti
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
different combinations and amount of these products, depending on organism
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
121% of the activity with laminaran
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
different combinations and amount of these products, depending on organism
?
laminaritriose + 2 H2O
3 D-glucose
-
-
-
-
?
laminaritriose + 2 H2O
3 D-glucose
-
-
complete hydrolysis within 2 h to yield glucose
-
?
laminaritriose + 2 H2O
3 D-glucose
-
-
complete hydrolysis within 2 h to yield glucose
-
?
laminaritriose + 2 H2O
3 D-glucose
-
-
-
?
laminaritriose + 2 H2O
3 D-glucose
-
-
-
?
laminaritriose + H2O
?
-
-
-
-
?
laminaritriose + H2O
?
-
-
-
-
?
laminaritriose + H2O
?
-
-
-
?
laminaritriose + H2O
?
-
-
-
?
laminaritriose + H2O
?
-
-
-
-
?
laminaritriose + H2O
?
-
-
-
-
?
laminaritriose + H2O
D-glucose + ?
-
-
-
-
?
laminaritriose + H2O
D-glucose + ?
-
97% of the activity with laminaribiose
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
D-glucose only
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
115% of the activity with laminaran
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
?
lentinan + H2O
?
-
-
-
-
?
lentinan + H2O
?
-
lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity
-
-
?
lentinan + H2O
?
-
-
-
-
?
lentinan + H2O
?
-
lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenin + H2O
D-glucose
-
-
-
?
lichenin + H2O
D-glucose
-
-
-
?
linear alpha-1,5-L-arabinan + H2O
?
-
-
-
?
linear alpha-1,5-L-arabinan + H2O
?
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
incompletely degraded
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
?
pachyman + H2O
D-glucose
Fungi imperfecti
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
pachyman + H2O
D-glucose
-
-
-
-
?
periodate-oxidized and reduced laminarin
glycerol + glycoaldehyde + D-glucose
-
periodate-oxidized and reduced
-
-
?
periodate-oxidized and reduced laminarin
glycerol + glycoaldehyde + D-glucose
-
periodate-oxidized and reduced
-
?
periodate-oxidized and reduced laminarin
glycerol + glycoaldehyde + D-glucose
-
periodate-oxidized and reduced
-
?
pustulan + H2O
?
-
-
-
-
?
pustulan + H2O
?
-
-
-
-
?
pustulan + H2O
?
-
41% of activity compared to laminaran
-
-
?
pustulan + H2O
D-glucose
-
-
-
-
?
pustulan + H2O
D-glucose
-
-
-
-
?
pustulan + H2O
D-glucose
-
-
-
-
?
pustulan + H2O
D-glucose
-
-
-
-
?
pustulan + H2O
D-glucose
-
-
-
-
?
pustulan + H2O
D-glucose
-
-
-
?
pustulan + H2O
D-glucose
-
-
-
?
salicin + H2O
?
-
-
-
-
?
salicin + H2O
?
-
-
-
-
?
schizophyllan + H2O
D-glucose + gentiobiose
-
-
-
?
schizophyllan + H2O
D-glucose + gentiobiose
-
-
-
-
?
sclerotan + H2O
D-glucose + gentiobiose
-
glucan from Sclerotinia sclerotiorum cell wall
-
-
?
sclerotan + H2O
D-glucose + gentiobiose
-
glucan from Sclerotinia sclerotiorum cell wall
-
?
sugar beet arabinan + H2O
?
-
-
-
?
sugar beet arabinan + H2O
?
-
-
-
?
wheat flour arabinoxylan + H2O
?
-
-
-
?
wheat flour arabinoxylan + H2O
?
-
-
-
?
zymosan A + H2O
D-glucose + ?
-
45% of the activity with laminarin
-
-
?
zymosan A + H2O
D-glucose + ?
-
45.4% of the activity with laminarin
-
-
?
additional information
?
-
-
no direct degradation activity with laminaribiose as a sole substrate, but the enzyme is capable of degrading it via transglycosylation reaction with laminaritriose
-
-
?
additional information
?
-
-
sAkLam33 substrate specificity amd mode of action, overview. The enzyme is inactive toward laminaribiose, starch, carboxymethylcellulose, agar, beta-1,4-mannan, beta-1,4-xylan, and alginic acid, and it is specific to beta-1,3-glucosyl linkages
-
-
?
additional information
?
-
-
the enzyme exhibits high substrate specificity, almost exclusively catalyzing beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose. Additionally, it shows transglycosylation activity
-
-
?
additional information
?
-
-
enzyme almost exclusively catalyzes beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose and acts as exoglucanase with transglycosylation activity. No substrates: avicel, pustulan, dextran, or amylose
-
-
?
additional information
?
-
-
the enzyme exhibits high substrate specificity, almost exclusively catalyzing beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose. Additionally, it shows transglycosylation activity
-
-
?
additional information
?
-
exgA expression is induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression
-
-
?
additional information
?
-
-
exgA expression is induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression
-
-
?
additional information
?
-
exgA expression is induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression
-
-
?
additional information
?
-
two surface loops, amino acids 36-47 and 101-106, might play a functional role after substrate binding. The loops could bind the enzyme to a glucan chain in the cell wall. Molecular dynamics and modelling of enzyme-substrate complexes, overview
-
-
?
additional information
?
-
requirement for double-sided CH/pi interactions at the +1 subsite, and necessity for phenylalanine rather than tyrosine or tryptophan. Carbohydrate-protein interactions at the +1 subsite of Exg provide the main contribution to the transition state interaction energy
-
-
?
additional information
?
-
the enzyme shows glycohydrolase and transglycosidase activities, substrate binding structures determined from mutant E292S complex crystal structures, detailed overview
-
-
?
additional information
?
-
no substrates: beechwood xylan, soluble starch, gum arabic, and larch arabinogalactan, 4-nitrophenyl alpha-D-xylopyranoside and 4-nitrophenyl beta-D-xylopyranoside
-
-
?
additional information
?
-
no substrates: beechwood xylan, soluble starch, gum arabic, and larch arabinogalactan, 4-nitrophenyl alpha-D-xylopyranoside and 4-nitrophenyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
no substrates: beechwood xylan, soluble starch, gum arabic, and larch arabinogalactan, 4-nitrophenyl alpha-D-xylopyranoside and 4-nitrophenyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
the enzyme is involved in mycoparasitism
-
-
?
additional information
?
-
-
enzyme can degrade cell walls of plant pathogens including Rhizoctonia solani, Gibberella zeae, Fusarium sp., Colletotrichum gloeosporioides and Phoma sp.
-
-
?
additional information
?
-
no substrate: periodate-oxidized laminarin, 4-nitrophenyl D-glucoside, laminaribiose
-
-
?
additional information
?
-
no substrate: periodate-oxidized laminarin, 4-nitrophenyl D-glucoside, laminaribiose
-
-
?
additional information
?
-
no substrates: barley beta-glucan or pustulan, cellobiose or gentiobiose, 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, 2-nitrophenyl-beta-D-galactopyranoside or 4-nitrophenyl-beta-D-glucuronide. The enzyme additionally shows transglycosylation activity
-
-
?
additional information
?
-
no substrates: barley beta-glucan or pustulan, cellobiose or gentiobiose, 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, 2-nitrophenyl-beta-D-galactopyranoside or 4-nitrophenyl-beta-D-glucuronide. The enzyme additionally shows transglycosylation activity
-
-
?
additional information
?
-
no activity with chitin, pustulan, carboxymethyl cellulose, soluble starch, pachyman, barley beta-glucan, periodate-oxidized laminarin, laminaribose, gentiobiose, cellobiose, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, 2-nitrophenyl beta-D-galactopyranoside, and 4-nitrophenyl beta-D-glucuronide
-
-
?
additional information
?
-
no activity with chitin, pustulan, carboxymethyl cellulose, soluble starch, pachyman, barley beta-glucan, periodate-oxidized laminarin, laminaribose, gentiobiose, cellobiose, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, 2-nitrophenyl beta-D-galactopyranoside, and 4-nitrophenyl beta-D-glucuronide
-
-
?
additional information
?
-
no substrates: barley beta-glucan or pustulan, cellobiose or gentiobiose, 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, 2-nitrophenyl-beta-D-galactopyranoside or 4-nitrophenyl-beta-D-glucuronide. The enzyme additionally shows transglycosylation activity
-
-
?
additional information
?
-
no substrates: barley beta-glucan or pustulan, cellobiose or gentiobiose, 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, 2-nitrophenyl-beta-D-galactopyranoside or 4-nitrophenyl-beta-D-glucuronide. The enzyme additionally shows transglycosylation activity
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additional information
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no substrate: periodate-oxidized laminarin, 4-nitrophenyl D-glucoside, laminaribiose
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no substrate: periodate-oxidized laminarin, 4-nitrophenyl D-glucoside, laminaribiose
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additional information
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the enzyme is activated by stress stimulus
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exo-1,3-beta-D-glucanases remove the glucose residues or gentiobiose (Glc-beta-1,6-Glc) from the non-reducing ends of beta-1,3-D-glucans, beta-1,3-1,6-D-glucans and laminarioligosaccharides
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enzyme activity with different substrates in wild-type and mutant strains, overview
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enzyme activity with different substrates in wild-type and mutant strains, overview
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ExgP is active only toward glucans containing beta-1,3-linkages, such as laminarin and laminaran, no or poor activity with curdran, carboxymethyl-curdlan, lentinan, beta-glucan from Hordeum vulgare, pusturan and carboxymethylcellulose
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exo-1,3-beta-glucanases cleave glucose residues from the non-reducing terminus of a beta-glucan chain
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no substrate: beta-1,4-glucans, beta-1,3/1,4-glucans or 4-nitrophenyl- and methylumbelliferyl-beta-D-glucopyranosides, carboxymethyl cellulose, avicel. Trace activity with pustulan
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the enzyme can hydrolyze laminaripentaose and laminaritetraose completely in 5 min to glucose and laminaribiose or laminaritriose, whereas overnight incubation is required for complete hydrolysis of laminaritriose, substrate specificity of the purified beta-1,3-glucanase, overview
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isoform Bglu3B exhibits a broad range of substrate specificity, catalyzing the cleavage of beta-1,2, beta-1,3, beta-1,4 and beta-1,6 linkages, in various oligosaccharides, to liberate glucose. Bglu3B shows relatively high activity toward laminaran and transglycosylation activity, enabling gentiobiose production. No substrates: 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, lactose, barley beta-glucan, Avicel, carboxymethyl cellulose, lichenan, birchwood xylan and chitin
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enzyme also catalyzes transglycoslation reactions
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enzyme prefers small substrates, has broad specificity for beta-1,3-linkages and beta-1,6-linkages
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no substrate: starch, chitin, chitosan
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no activity with chitin, chitosan, and cellulose
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no activity with chitin, chitosan, and cellulose
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hydrolysis with inversion of the anomeric configuration, substrate subsite structure and model of enzyme binding site
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PAEXG1 and PAEXG2 are separately and sequentially disrupted. The resulting mutant strains show a significantly reduced efficiency of biocontrol of Botrytis cinerea. When applied to wounded apple fruit, the calculated protection level dropping from 71% (parental strain) to 8% (mutated strain). Exo-beta-1,3-glucanases play a role in the biological control of Botrytis cinerea on apple
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additional information
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PAEXG1 and PAEXG2 are separately and sequentially disrupted. The resulting mutant strains show a significantly reduced efficiency of biocontrol of Botrytis cinerea. When applied to wounded apple fruit, the calculated protection level dropping from 71% (parental strain) to 8% (mutated strain). Exo-beta-1,3-glucanases play a role in the biological control of Botrytis cinerea on apple
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