construction of a exgA disruptant and an exgA-overexpressing mutant. The exgA-overexpressing mutant shows higher mycelial growth rate and conidial efficiency than the exgA disruptant when beta-1,3-glucan is supplied as sole carbon source. No difference in phenotype is observed between them
gene exgP, phylogenetic analysis, subcloning in Escherichia coli strain JM109, heterologous secretory expression in Aspergillus oryzae, the recombinant enzyme is secreted to the culture medium
gene WsEXG1, cloned from from both the genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, subcloning of a His6-tagged enzyme in Escherichia coli strain DH5alpha, functional overexpression in Yarrowia lipolytica strain Po1h