3.2.1.54: cyclomaltodextrinase
This is an abbreviated version!
For detailed information about cyclomaltodextrinase, go to the full flat file.
Word Map on EC 3.2.1.54
-
3.2.1.54
-
starch
-
cyclodextrins
-
maltose
-
maltodextrins
-
ceramidase
-
neopullulanase
-
transglycosylation
-
thermoactinomyces
-
alpha-amylases
-
maltogenic
-
maltotriose
-
glucanotransferase
-
amylolytic
-
acarbose
-
amylases
-
maltoheptaose
-
malto-oligosaccharides
-
cgtases
-
cyclomaltodextrins
-
maltotetraose
-
panose
-
beta-cd
-
alkalophilic
-
amylomaltase
-
alpha-cd
-
anoxybacillus
-
biotechnology
-
synthesis
-
food industry
-
analysis
- 3.2.1.54
- starch
- cyclodextrins
- maltose
- maltodextrins
- ceramidase
- neopullulanase
-
transglycosylation
-
thermoactinomyces
- alpha-amylases
-
maltogenic
- maltotriose
-
glucanotransferase
-
amylolytic
- acarbose
- amylases
- maltoheptaose
- malto-oligosaccharides
- cgtases
- cyclomaltodextrins
- maltotetraose
- panose
- beta-cd
-
alkalophilic
- amylomaltase
-
alpha-cd
- anoxybacillus
- biotechnology
- synthesis
- food industry
- analysis
Reaction
Synonyms
AfCda13, AglB, alpha-amylase, alpha-amylase II, CD-/pullulan-hydrolyzing enzyme, CD-ase, CD-degrading enzyme, CD-hydrolyzing amylase, CDA, CDase, CDase I-5, CMD, cyclodextrinase, cycloheptaglucanase, cyclohexaglucanase, cyclomaltodextrin dextrin-hydrolase, Cyclomaltodextrin hydrolase, decycling, cyclomaltodextrinase, CymH, cytoplasmic decycling maltodextrinase, EC 3.2.1.12, Env cda13A, FspCMD, H-17 CDase, H-17 thermostable CDase, LLCD, LsCda13, Lsp26X-Mdase, maltodextrin glucosidase, More, neopullulanase, PFTA, RA.04, thermophilic CDase, TK1770, TVA II
ECTree
Advanced search results
Temperature Stability
Temperature Stability on EC 3.2.1.54 - cyclomaltodextrinase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
100
25 - 50
the enzyme remains stable after 30 min incubation at 45°C and loses all its activity when incubated for 30 min at 50°C. The temperature at which the enzyme loses 50% of its activity with 2 mM calcium is 48.5°C. The enzyme remains 60% active after 24 h at 25 or 40°C but is 20% active at 0°C
40
42
-
MalZ loses its native conformation and undergoes aggregation during incubation at 42°C., thermoprotection is achieved by proline, glycerol, dimethylsulfoxide or trimethylamine-N-oxide
57
-
complete loss of activity after 20 min without calcium, completely stable after 120 min in presence of 1 mM calcium
60
60 - 70
-
enzyme half-life 402 min at 60°C, 180 min at 65°C, 75 min at 70°C
65
75 - 95
-
the enzyme retains more than 90% activity after 8 h at 75-85°C. At 95°C, the enzyme shows about 77%, 45%, 30% and less than 20% activity after 1 h, 2 h, 4 h and 8 h, respectively
85
90
93
purified enzyme, melting temperature, differential scanning calorimetry and differential scanning fluorimetry analysis
95
additional information
100
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 65% activity within 60 min
half-life: 20 min. Thermal inactivation is not significantly affected by addition of calcium
60
-
half-life: 4 min, in absence of calcium. 50% loss of activity after 120 min in presence of 1 mM calcium
65
-
incubation for 1 h led to a 15% loss of activity
85
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, completely stable for 120 min
90
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 15-20% activity within 120 min
90
-
half-inactivation time of AglB(2Met) at 90°C is 5 min, for AglB* and AglB(1Met) 3 h
95
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 25% activity within 120 min
60 - 80
additional information
-
half-life at 60°, 65°, 70° and 80°C is 600, 480, 30 and 12 min, respectively, independent on the pH and the presence of Ca2+