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3.2.1.54: cyclomaltodextrinase

This is an abbreviated version!
For detailed information about cyclomaltodextrinase, go to the full flat file.

Word Map on EC 3.2.1.54

Reaction

Cyclomaltodextrin
+
H2O
=
linear maltodextrin

Synonyms

AfCda13, AglB, alpha-amylase, alpha-amylase II, CD-/pullulan-hydrolyzing enzyme, CD-ase, CD-degrading enzyme, CD-hydrolyzing amylase, CDA, CDase, CDase I-5, CMD, cyclodextrinase, cycloheptaglucanase, cyclohexaglucanase, cyclomaltodextrin dextrin-hydrolase, Cyclomaltodextrin hydrolase, decycling, cyclomaltodextrinase, CymH, cytoplasmic decycling maltodextrinase, EC 3.2.1.12, Env cda13A, FspCMD, H-17 CDase, H-17 thermostable CDase, LLCD, LsCda13, Lsp26X-Mdase, maltodextrin glucosidase, More, neopullulanase, PFTA, RA.04, thermophilic CDase, TK1770, TVA II

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.54 cyclomaltodextrinase

Temperature Stability

Temperature Stability on EC 3.2.1.54 - cyclomaltodextrinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
the enzyme remains stable after 30 min incubation at 45°C and loses all its activity when incubated for 30 min at 50°C. The temperature at which the enzyme loses 50% of its activity with 2 mM calcium is 48.5°C. The enzyme remains 60% active after 24 h at 25 or 40°C but is 20% active at 0°C
37
-
24 h, significant loss of activity
4 - 55
-
48 h, little loss of activity
42
-
MalZ loses its native conformation and undergoes aggregation during incubation at 42°C., thermoprotection is achieved by proline, glycerol, dimethylsulfoxide or trimethylamine-N-oxide
45
-
stable at temperatures below , pH 7
50
-
stable at temperatures below
57
-
complete loss of activity after 20 min without calcium, completely stable after 120 min in presence of 1 mM calcium
60 - 70
-
enzyme half-life 402 min at 60°C, 180 min at 65°C, 75 min at 70°C
67
-
complete loss of activity despite presence of calcium
75 - 95
-
the enzyme retains more than 90% activity after 8 h at 75-85°C. At 95°C, the enzyme shows about 77%, 45%, 30% and less than 20% activity after 1 h, 2 h, 4 h and 8 h, respectively
93
purified enzyme, melting temperature, differential scanning calorimetry and differential scanning fluorimetry analysis
additional information