3.2.1.49: alpha-N-acetylgalactosaminidase

This is an abbreviated version!
For detailed information about alpha-N-acetylgalactosaminidase, go to the full flat file.

Word Map on EC 3.2.1.49

Reaction

alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide
+
H2O
=
D-GalNAc
+
beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide

Synonyms

alpha-acetylgalactosaminidase, N-acetyl-alpha-D-galactosaminidase, N-acetyl-alpha-galactosaminidase, 4-nitrophenyl-alpha-N-acetylgalactosaminidase, 2-acetamido-2-deoxy-alpha-D-galactoside acetamidodeoxygalactohydrolase, exo-alpha-N-acetylgalactosaminidase, alpha-galactosidase B, AglA, alpha-NAGAL, alpha-GalNAc, alpha-NaGalase, alpha-N-galactosaminidase IV, NaGalase, alpha-N-acetylgalactosaminidase blood group A2 degrading enzyme, alpha-NAGA, envelope glycoprotein gp160, NagA, alpha-GalNAcase I, alpha-GalNAcase II, alpha-GalNAcase, alpha-GalNAc-ase, alpha-N acetylgalactosaminidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.49 alpha-N-acetylgalactosaminidase

Storage Stability

Storage Stability on EC 3.2.1.49 - alpha-N-acetylgalactosaminidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 6 months
-20°C, about 5% loss of activity
-
-20°C, acidified plasma, at least one year
-
-20C, lyophilized, several years
0°C, 1 month
-
0°C, sodium citrate buffer, bovine serum albumin, 2 weeks, pH 4.2
-
20°C, stable for 1 week
-
4°C, 12 months
-
4°C, 50 mM citrate-phosphate buffer within the pH range 2-4, stable for several weeks
4°C, enzyme separation buffer containing 3 mM sodium azide, 25-31 months
-
4°C, pH 1.5-4.0, stable for several months
-
4°C, sterile condition, stable for any length of time
-
the purified preparation of the enzyme can be stored frozen for a month and retains 100% of its activity after thawing
the purified wild-type and N201Q mutant proteins expressed in insect cells retain nearly full activity for months at 4°C, but the CHO-expressed material loses most of its activity within 72 h