3.2.1.49: alpha-N-acetylgalactosaminidase

This is an abbreviated version!
For detailed information about alpha-N-acetylgalactosaminidase, go to the full flat file.

Word Map on EC 3.2.1.49

Reaction

alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide
+
H2O
=
D-GalNAc
+
beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide

Synonyms

alpha-acetylgalactosaminidase, N-acetyl-alpha-D-galactosaminidase, N-acetyl-alpha-galactosaminidase, 4-nitrophenyl-alpha-N-acetylgalactosaminidase, 2-acetamido-2-deoxy-alpha-D-galactoside acetamidodeoxygalactohydrolase, exo-alpha-N-acetylgalactosaminidase, alpha-galactosidase B, AglA, alpha-NAGAL, alpha-GalNAc, alpha-NaGalase, alpha-N-galactosaminidase IV, NaGalase, alpha-N-acetylgalactosaminidase blood group A2 degrading enzyme, alpha-NAGA, envelope glycoprotein gp160, NagA, alpha-GalNAcase I, alpha-GalNAcase II, alpha-GalNAcase, alpha-GalNAc-ase, alpha-N acetylgalactosaminidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.49 alpha-N-acetylgalactosaminidase

Purification

Purification on EC 3.2.1.49 - alpha-N-acetylgalactosaminidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme from salivary gland cells
-
enzyme mutant from cell culture by dialysis, ammonium sulfate fractionation, hydrophobic interaction, cation exchange, and anion exchange chromatography
native enzyme
-
native enzyme from cell culture supernatant by three chromatographic steps to homogeneity, recombinant enzyme from Saccharomyces cerevisiae to homogeneity 105.9fold by hydrophobic interaction chromatography, ultrafiltration, cation exchange chromatography, gel filtration, and anion exchange chromatography
native enzyme from placenta by 4-aminophenyl thio-beta-D-galactose-ceramide hexoside-resin affinity
-
native isozymes alpha-GalNAcase I and alpha-GalNAcase II from liver
-
native isozymes alpha-GalNAcase I and II are isolated from liver homogenate by ammonium sulfate fractionation and separated from each other by gel filtration. Then they are further individually purified by cation exchange chromatography and gel filtration, and additionally by hydroxylapatite chromatography for isozyme alpha-GalNAcase II. Isozyme alpha-GalNAcase I is purified 440fold, isozyme alpha-GalNAcase II 544fold
-
partially
recombinant enzyme
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein