Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.49: alpha-N-acetylgalactosaminidase

This is an abbreviated version!
For detailed information about alpha-N-acetylgalactosaminidase, go to the full flat file.

Word Map on EC 3.2.1.49

Reaction

alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide
+
H2O
=
D-GalNAc
 +
beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide

Synonyms

2-acetamido-2-deoxy-alpha-D-galactoside acetamidodeoxygalactohydrolase, 4-nitrophenyl-alpha-N-acetylgalactosaminidase, AglA, alpha-acetylgalactosaminidase, alpha-galactosidase B, alpha-GalNAc, alpha-GalNAc-ase, alpha-GalNAcase, alpha-GalNAcase I, alpha-GalNAcase II, alpha-N acetylgalactosaminidase, alpha-N-acetylgalactosaminidase blood group A2 degrading enzyme, alpha-N-galactosaminidase IV, alpha-NAGA, alpha-NAGAL, alpha-NaGalase, envelope glycoprotein gp160, exo-alpha-N-acetylgalactosaminidase, N-acetyl-alpha-D-galactosaminidase, N-acetyl-alpha-galactosaminidase, NagA, NaGalase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.49 alpha-N-acetylgalactosaminidase

Cloned

Cloned on EC 3.2.1.49 - alpha-N-acetylgalactosaminidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as inactive enzyme in inclusion bodies, expression in Pichia pastoris strain X33 is not successful, heterologous overexpression in Saccharomyces cerevisiae strain W303-1A without enzyme secretion
expressed in Pichia pastoris
expression in B-lymphoblast cell line
-
expression in bacteria, rabbit reticulocyte lysate and yeast
-
expression in Chines hamster ovary cells
-
expression in CHO and COS-1 cells. Expression of wild-type and mutant enzymes in Escherichia coli as insoluble proteins, and in Kluyveromyces lactis as soluble hyperglycosylated proteins. Expression of wild-type and mutant enzymes in Trichoplusia ni Tn5 insect cells using the baculovirus transfection system leads to suitable proteins
expression in Cos-1 cells
expression in Escherichia coli
expression in Saccharomyces cerevisiae
expression in yeast Pichia pastoris
-
expression of aagA gene in Escherichia coli, resulting in recombinants exhibiting high-level expression of the expected activity
expression of the engineered enzyme in CHO cells
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
the gene encoding the clostridial enzyme is cloned in an Escherichia coli T7 expression system
-