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3.2.1.41: pullulanase

This is an abbreviated version!
For detailed information about pullulanase, go to the full flat file.

Word Map on EC 3.2.1.41

Reaction

(Glcalpha(1-4)Glcalpha(1-4)Glcalpha(1-6))n
+
H2O
=
alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose
+
(Glcalpha(1-4)Glcalpha(1-4)Glcalpha(1-6))n-1

Synonyms

1,4-alpha-D-glucan glucanohydrolase, Alpha-dextrin endo-1,6-alpha-glucosidase, amylase-pullulanase, amylopectin 6-glucanohydrolase, amylopullulanase, AmyX, Apu, ApuK885, ApuM957, Ask, BaPul13A, BDPulA324, BmPul, BNPulA324, CAC60157, debranching enzyme, EC 3.2.1.69, glucanohydrolase, amylopectin 6-, limit dextrinase, More, Nostoc punctiforme debranching enzyme, NPDE, PbPulA, Pcal-1616, PelBsp-PulA, PfAPU, Promozyme 200 L, PUL US105, Pul13A, Pul3YH5, PulA, PulASK, PulB, pulGT, Pullulan 6-glucanohydrolase, pullulan-6-glucanohydrolase, pullulanase, pullulanase type 1, pullulanase type I, pullulanase type II, PulWB42, R-enzyme, Saci_1162, TPApu, TTC1198, TTHpu, type II pullulanase, ZUP1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.41 pullulanase

Molecular Weight

Molecular Weight on EC 3.2.1.41 - pullulanase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
100000 - 105000
-
gel filtration, SDS-PAGE
105000
-
gel filtration
106063
calculated from sequence
109000
-
x * 109000, SDS-PAGE
110000
112000
115000
-
1 * 115000, gel electrophoresis
115000 - 116000
118000
-
precursor protein, gel electrophoresis, amino acid analysis
119000
-
1 * 119000, SDS-PAGE
120000
125000
-
gel filtration
126000
-
protease-released enzyme, gel filtration
135000
-
gel filtration
14000
-
x * 14000, SDS-PAGE
141000
-
extracellular enzyme, gel filtration
143000
-
gel electrophoresis, amino acid analysis
148600
x * 148600, calculated from amino acid sequence
218000
-
1 * 218000, gel electrophoresis
218000 - 220000
-
gel filtration, gel electrophoresis, amino acid analysis
240000
-
gel filtration, SDS-PAGE
435000
gel filtration
50000
x * 50000, recombinant enzyme after heat treatment in denaturing buffer at 70°C or below, SDS-PAGE
53000 - 56000
-
gel filtration, gel electrophoresis
65000
-
gel filtration, SDS-PAGE
67000
-
gel filtration
70000
-
gel filtration
73000
-
x * 73000, SDS-PAGE
73900
-
x * 73900, SDS-PAGE
74000
x * 74000, deduced from gene sequence, mature protein
75093
calculated from sequence
77000
-
gel filtration
776000
-
3 * 776000, SDS-PAGE
79000
-
gel filtration
80000
80000 - 83000
-
gel filtration, gel electrophoresis
80000 - 90000
-
gel filtration, sedimentation equilibrium
80370
calculated from sequence
81000
-
x * 81000, SDS-PAGE
81100
-
x * 81100, calculated from sequence
83000
-
gel electrophoresis
85000
-
gel filtration
90000
92000
-
gel electrophoresis
93000
x * 93000, SDS-PAGE
96000
x * 96000, mature protein, deduced from gene sequence
97000
-
gel filtration, SDS-PAGE