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2-mercaptoethanol
5 mM, 51% residual activity
ammonium sulfate
0.5 mM, 36% residual activity
CaCl2
1 mM, 1.8fold activation. 5 mM, 66% loss of activity. 10 mM, 97% loss of activity
CoCl2
1 mM, 97% inhibition
EGTA
-
1-10 mM, 65-70% inhibition
gamma-cyclodextrin
-
10 mM, 20% loss of activity
iodoacetate
-
1 M, 10% loss of activity
NH4+
EPZ37738
2 mM, 49% of initial activtiy
phenylmethylsulfonyl fluoride
-
partially
pullulan
-
substrate inhibition above 0.1%
sodium dodecylsulfate
1 mM, no residual activity
Sodium fluoride
-
1 M, 10% loss of activity
ZnCl2
5 mM, 51% residual activity
ZnSO4
1 mM, complete inhibition
1,10-phenanthroline
-
-
Ag+
-
-
Ag+
1 mM, 84% loss of activity
Ag+
-
0.1 M, 50% loss of activity
alpha-cyclodextrin
-
10 mM, 10% loss of activity
alpha-cyclodextrin
1%, 99% inhibition
alpha-cyclodextrin
-
1-10 mM, about 85% inhibition
Ba2+
EPZ37738
2 mM, 54% of initial activtiy
beta-cyclodextrin
-
10 mM, 90% loss of activity. Activity of pullulanase remains at more than 40% when mixtures containing beta-cyclodextrin and sodium benzoate are added. Side chain groups lead to decrease the interaction between hydrophobic cavities of beta-cyclodextrin and pullulanase molecules
beta-cyclodextrin
-
1-10 mM, 80-85% inhibition
Ca2+
-
-
Ca2+
-
5 mM, starch-hydrolyzing activity is inhibited by about 30%, hydrolytic activity against glycogen is slightly inhibited
Ca2+
-
5 mM, starch-hydrolyzing activity is inhibited by about 14%, hydrolytic activity against glycogen is slightly inhibited
Co2+
EPZ37738
2 mM, 15% of initial activtiy
Co2+
-
5 mM, at least 60% inhibition
Co2+
1 mM, 18% residual activity
Cr3+
-
-
Cr3+
5 mM, 34% inhibition, pH 6.5, 70°C
Cu2+
EPZ37738
2 mM, 8% of initial activtiy
Cu2+
5 mM, 67% residual activity
Cu2+
2 mM, 79% residual activity
Cu2+
-
5 mM, at least 60% inhibition
Cu2+
1 mM, 82% loss of activity
Cu2+
1 mM, complete inactivation
Cu2+
5 mM, complete inhibition, pH 6.5, 70°C
CuCl2
5 mM, no residual activity
CuCl2
1 mM, complete inhibition
cyclodextrin
-
-
Cyclodextrins
-
-
-
Cyclodextrins
-
Schardinger dextrins
-
EDTA
EPZ37738
2 mM, 68% of initial activtiy
EDTA
5 mM, 63% residual activity
EDTA
5 mM, 72% residual activity
EDTA
5 mM, complete inhibition
EDTA
-
1-10 mM, about 70% inhibition
Fe2+
EPZ37738
2 mM, 6% of initial activtiy
Fe3+
-
-
Guanidine HCl
-
unfolding of the enzyme
Guanidine HCl
2.0 mM, about 55% inhibition
Guanidine HCl
-
at 0.25 M
Hg2+
-
-
Hg2+
-
complete inactivation
Hg2+
-
5 mM, at least 60% inhibition
Hg2+
1 mM, 99% loss of activity
Hg2+
1 mM, complete inactivation
Hg2+
5 mM, complete inhibition, pH 6.5, 70°C
Hg2+
-
0.1 M, 70% loss of activity
iodoacetamide
-
-
Mg2+
5 mM, 90% residual activity
Mg2+
-
reduces the thermoactivity of the enzymes below the level seen in the absence of added metal
Mg2+
-
reduces the thermoactivity of the enzymes below the level seen in the absence of added metal
Mn2+
EPZ37738
2 mM, 40% of initial activtiy
Mn2+
1 mM, 15% residual activity
N-bromosuccinimide
-
inhibitory at 10mM
N-bromosuccinimide
0.01%, no residual activity
N-bromosuccinimide
-
at 5 mM
N-bromosuccinimide
complete inactivation
Ni2+
EPZ37738
2 mM, 47% of initial activtiy
Ni2+
1 mM, 18% residual activity
Ni2+
5 mM, 31% inhibition, pH 6.5, 70°C
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
Pb2+
-
-
Pb2+
5 mM, 75% inhibition, pH 6.5, 70°C
SDS
5 mM, 46% residual activity
SDS
5-50 mM, 25-85% inhibition
SDS
1%, complete inactivation
SDS
10 mM, 96% inhibition, pH 6.5, 70°C
Sn2+
-
-
Sn2+
1 mM, 87% loss of activity
Sodium dodecyl sulfate
-
-
Sodium dodecyl sulfate
-
-
Sodium dodecyl sulfate
-
-
Sodium dodecyl sulfate
-
-
Sodium dodecyl sulfate
-
-
Sr2+
EPZ37738
2 mM, 73% of initial activtiy
Triton X-100
EPZ37738
1% v/v, 72% of initial activtiy
Urea
EPZ37738
2 mM, 57% of initial activtiy
Urea
4 M, 81% residual activity
Urea
2 M, complete inhibition
Zn2+
EPZ37738
2 mM, 22% of initial activtiy
Zn2+
2 mM, 48% residual activity
Zn2+
-
5 mM, at least 60% inhibition
Zn2+
1 mM, 53% residual activity
Zn2+
5 mM, 58% inhibition, pH 6.5, 70°C
additional information
-
the interaction between cyclodextrins and pullulanase is closely related to the sizes of hydrophobic cavities within cyclodextrins
-
additional information
-
EDTA has no effect, phenylmethanesulfonyl fluoride has no effect
-
additional information
-
EDTA has no effect, phenylmethanesulfonyl fluoride and diisopropylflurophosphate have almost no effect
-
additional information
not inhibitory: iodoacetamide, Triton X-100, Mn2+, Mg2+, Ca2+
-
additional information
-
not inhibitory: iodoacetamide, Triton X-100, Mn2+, Mg2+, Ca2+
-
additional information
enzyme retains 99, 89, and 54% of its activity at 10% concentration of Triton-X100, Tween 20, and SDS, respectively. The enzyme retains 80.4 and 93.7% activity in the presence of commercial detergents Rika (7.5% v/v) and Fadisheh (2.5% w/v), respectively
-
additional information
-
EDTA has no effect
-
additional information
not inhibitory: EDTA
-
additional information
-
not inhibitory: EDTA
-