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3.2.1.41: pullulanase

This is an abbreviated version!
For detailed information about pullulanase, go to the full flat file.

Word Map on EC 3.2.1.41

Reaction

(Glcalpha(1-4)Glcalpha(1-4)Glcalpha(1-6))n
+
H2O
=
alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose
 +
(Glcalpha(1-4)Glcalpha(1-4)Glcalpha(1-6))n-1

Synonyms

1,4-alpha-D-glucan glucanohydrolase, Alpha-dextrin endo-1,6-alpha-glucosidase, amylase-pullulanase, amylopectin 6-glucanohydrolase, amylopullulanase, AmyX, Apu, ApuK885, ApuM957, Ask, BaPul13A, BDPulA324, BmPul, BNPulA324, CAC60157, debranching enzyme, EC 3.2.1.69, glucanohydrolase, amylopectin 6-, limit dextrinase, More, Nostoc punctiforme debranching enzyme, NPDE, PbPulA, Pcal-1616, PelBsp-PulA, PfAPU, Promozyme 200 L, PUL US105, Pul13A, Pul3YH5, PulA, PulASK, PulB, pulGT, Pullulan 6-glucanohydrolase, pullulan-6-glucanohydrolase, pullulanase, pullulanase type 1, pullulanase type I, pullulanase type II, PulWB42, R-enzyme, Saci_1162, TPApu, TTC1198, TTHpu, type II pullulanase, ZUP1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.41 pullulanase

Cloned

Cloned on EC 3.2.1.41 - pullulanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3) pLysS cells
expressed in Escherichia coli using pHsh and pET28a vectors. The pHsh expression system produces a 3.6fold higher recombinant pullulanase than pET28a
expression in Bacillus subtilis
expression in Brevibacillus choshinensis
-
expression in Escherichia coli
expression in Escherichia coli and Bacillus subtilis
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli, connection to the lipase A signal peptide of Bacillus subtilis strain leads to an efficient secretion of an active form enzyme on the periplasmic fraction, fusion to the amylase signal sequence of the Bacillus stearothermophilus US100 strain leads to an efficient secretion of this natively cytoplasmic enzyme as an active form in the supernatant
-
expression of His-tagged NPDE in Escherichia coli strain MC1061
-
expression of wild-type and mutant enzymes in Escherichia coli BL21 (DE3) cells
heterologously expressed in Escherichia coli
heterologously expressed in Escherichia coli as an intracellular soluble protein
homologously expressed in Sulfolobus acidocaldarius
overexpression in Escherichia coli
-
quantitative real-time PCR analysis of the expression pattern of the OsPUL gene from Oryza sativa ssp. indica cv. 9311 encoding a pullulanase-type debranching enzyme in different tissues as well as in seeds at different developmental stages, the OsPUL promoter actively functions in the late stage of seed germination, overview. Transcriptional profiling and regulatory elements of endogenous OsPUL gene in rice