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3.2.1.40: alpha-L-rhamnosidase

This is an abbreviated version!
For detailed information about alpha-L-rhamnosidase, go to the full flat file.

Word Map on EC 3.2.1.40

Reaction

4',5,7-trihydroxyflavanone 7-rhamnoglucoside
+
H2O
=
4',5,7-trihydroxyflavanone 7-glucoside
 +
alpha-L-rhamnose

Synonyms

alpha-L-rhamnosidase, alpha-L-rhamnosidase A, alpha-L-rhamnosidase B, alpha-L-rhamnosidase N, alpha-L-rhamnosidase Ram A, alpha-L-rhamnosidase T, alpha-RHA, AoRha, AorhaA, BtRha, BtRha78A, DtRha, EC 3.2.1.66, GH106 alpha-L-rhamnosidase, gypenoside-alpha-L-rhamnosidase, KoRha, L-rhamnosidase, More, naringinase, pnp-rhamnohydrolase, RamA, RHA-P, RhaA, RhaB, RhaB1, RhaB2, RhaL1, Rham, rhamnosidase, alpha -L-, RhmA, saponin-alpha-L-rhamnosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.40 alpha-L-rhamnosidase

pH Stability

pH Stability on EC 3.2.1.40 - alpha-L-rhamnosidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 7
-
stable between, 21% loss of activity after 24 h at pH 2.0
136381
3 - 11
24 h, 4°C, more than 45% of its enzymatic activity is retained
750829
3 - 5.5
-
RhaB retains over 85% of maximal activity
665264
3 - 7
-
purified enzyme, stable
666777
3 - 8
-
-
136392
3 - 9
3.5
-
naringinase complex, unstable below
695677
3.5 - 7
-
stable in the pH range 3.5-7.0
750853
4 - 5.5
-
RhaA retains over 85% of maximal activity
665264
4 - 8
-
12 h, 4°C, most of its activity is retained
750590
4.5 - 5
-
naringinase complex, most stable within this range
695677
5
1 h, 40 min, enzyme retains 75% of its initial activity
749441
5 - 7
-
-
654992
5 - 8
stable in the pH range 5.0-8.0 at 45°C for 4 h over 60%
750024
5.5 - 9
7.5 - 9
-
24 h, without substrate, 95% residual activity
715802
8.6 - 9
the activity of BtRha78A is enhanced significantly after the incubation in alkaline environment. The enzyme retains 142% and 133% of initial activity after the incubation of 1 h at 40 °C in 50 mM glycine-NaOH buffer (pH 8.6 and pH 9.0), respectively
749441
9 - 10
-
24 h, the enzyme is most stable between pH 9.0 and pH 10.0
750025