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3.2.1.40: alpha-L-rhamnosidase

This is an abbreviated version!
For detailed information about alpha-L-rhamnosidase, go to the full flat file.

Word Map on EC 3.2.1.40

Reaction

4',5,7-trihydroxyflavanone 7-rhamnoglucoside
+
H2O
=
4',5,7-trihydroxyflavanone 7-glucoside
 +
alpha-L-rhamnose

Synonyms

alpha-L-rhamnosidase, alpha-L-rhamnosidase A, alpha-L-rhamnosidase B, alpha-L-rhamnosidase N, alpha-L-rhamnosidase Ram A, alpha-L-rhamnosidase T, alpha-RHA, AoRha, AorhaA, BtRha, BtRha78A, DtRha, EC 3.2.1.66, GH106 alpha-L-rhamnosidase, gypenoside-alpha-L-rhamnosidase, KoRha, L-rhamnosidase, More, naringinase, pnp-rhamnohydrolase, RamA, RHA-P, RhaA, RhaB, RhaB1, RhaB2, RhaL1, Rham, rhamnosidase, alpha -L-, RhmA, saponin-alpha-L-rhamnosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.40 alpha-L-rhamnosidase

Temperature Range

Temperature Range on EC 3.2.1.40 - alpha-L-rhamnosidase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
30 - 70
30°C: about 65% of maximal activity, 70°C: about 55% of maximal activity
32 - 45
32°C: about 55% of maximal activity, 45°C: about 65% of maximal activity
35 - 65
35°C: about 50% of maximal activity, 65°C: about 70% of maximal activity
35 - 75
-
-
37 - 90
hydrolysis of 4-nitrophenyl-alpha-L-rhamnopyranoside at 37°C is catalyzed at 17% of the activity compared to that at 90°C
4 - 40
-
activity measured, 6% activity maintained at 4°C, thermo-inactivation above 50°C
40 - 60
40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity, hydrolysis of 4-nitrophenyl alpha-L-rhamnopyranoside
40 - 70
-
40°C: about 55% of maximal activity, 70°C: about 30% of maximal activity
40 - 80
low activity above
45 - 75
50 - 60
50 - 70
65 - 75
65°C: temperature-optimum of wild-type enzyme and mutant enzymes D594Q and G827K/D594Q. At 70 °C, the wild type enzyme retains 88.4% of its maximum activity, whereas the D594Q and G827K/D594Q mutant enzymes retain 96.6% and 98.5% of their maximum activities, respectively. At 75°C, the wild-type enzyme retains 48.5% of its maximum activity, whereas the D594Q and G827K/D594Q mutant enzymes retain 71.2% and 81.1% of their maximum activities, respectively
70
74% of maximum activity
80
36% of maximum activity