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3.2.1.40: alpha-L-rhamnosidase

This is an abbreviated version!
For detailed information about alpha-L-rhamnosidase, go to the full flat file.

Word Map on EC 3.2.1.40

Reaction

4',5,7-trihydroxyflavanone 7-rhamnoglucoside
+
H2O
=
4',5,7-trihydroxyflavanone 7-glucoside
 +
alpha-L-rhamnose

Synonyms

alpha-L-rhamnosidase, alpha-L-rhamnosidase A, alpha-L-rhamnosidase B, alpha-L-rhamnosidase N, alpha-L-rhamnosidase Ram A, alpha-L-rhamnosidase T, alpha-RHA, AoRha, AorhaA, BtRha, BtRha78A, DtRha, EC 3.2.1.66, GH106 alpha-L-rhamnosidase, gypenoside-alpha-L-rhamnosidase, KoRha, L-rhamnosidase, More, naringinase, pnp-rhamnohydrolase, RamA, RHA-P, RhaA, RhaB, RhaB1, RhaB2, RhaL1, Rham, rhamnosidase, alpha -L-, RhmA, saponin-alpha-L-rhamnosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.40 alpha-L-rhamnosidase

Purification

Purification on EC 3.2.1.40 - alpha-L-rhamnosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
buckwheat, partial
-
expression in Escherichia coli, strain BL21(DE3)
from cells grown on rhamnose as sole carbon source
-
HiTrap chelating column chromatography, gel filtration
native and recombinant proteins, gel filtration, SDS-PAGE
native enzyme from strain FFDCL-90, by ammonium sulfate fractionation and ion exchange chromatography
-
of the recombinant protein
-
recombinant enzyme 63fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography
recombinant enzyme 85fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography
recombinant protein, gel filtration, SDS-PAGE
recombinant Rham from Escherichia coli
saracen corn
-
to homogeneity
-