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3.2.1.18: exo-alpha-sialidase

This is an abbreviated version!
For detailed information about exo-alpha-sialidase, go to the full flat file.

Word Map on EC 3.2.1.18

Reaction

colominic acid
+
H2O
=
sialic acid
 +
lactose

Synonyms

acetylneuraminidase, Acetylneuraminyl hydrolase, acid sialidase, acylneuraminyl glycohydrolase, alpha-N-acylneuraminate glycohydrolase, alpha-neuraminidase, alpha-sialidase, alpha2,6-sialidase, alpha2,6-sialyltransferase, alpha2,6-trans-sialidase, Am0705, Am0707, Am1757, Am2085, Cytosolic sialidase, DELTA15Pd2,6ST, endo-N, endo-N-acylneuraminidase, endo-sialidase, endoNF, endosialidase, exo-alpha-sialidase, exo-sialidase, G9 sialidase, Ganglioside sialidase, ganglioside-specific sialidase, GH33C, glycosyl hydrolase, haemagglutinin-neuraminidase protein, hemagglutinin-neuraminidase, hemagglutinin-neuraminidase glycoprotein, HN, HsNEU2, IID sialidase, KDNase, Lysosomal sialidase, Major 85 kDa surface antigen, Major surface antigen, Membrane sialidase, MmNEU3, More, Mouse skeletal muscle sialidase, MSS, MTS, mucopolysaccharide N-acetylneuraminylhydrolase, Murine thymic sialidase, N-acetyl-alpha-neuraminidase, N-acetyl-alpha-neuraminidase 1, N-acetyl-alpha-neuraminidase 2, N-acetyl-alpha-neuraminidase 3, N-acetyl-alpha-neuraminidase 4, N-acetylneuraminosyl glycohydrolase, N-acylneuraminate glycohydrolase, N-acylneuraminosyl glycohydrolase, NA, NA1, NA2, NAN1, NanA, NANase, NanB, NanC, NanH, NanI, NanICD, NanJ, NanPs, Neu, NEU1, NEU2, Neu3, Neu3a, Neu3d, Neu3e, NEU4, NEU4 long, NEU4 short, NEU4L, Neu4L sialidase, neuramindase, neuraminidase, neuraminidase 1, neuraminidase 2, neuraminidase 3, neuraminidase-1, PA2794, SA85-1.1 protein, SA85-1.2 protein, SA85-1.3 protein, SiaBb1, sialidase, sialidase 4, sialidase II, sialidase Neu2, sialidase-2, sialidase-3, sialidase-4, sialidase/neuraminidase mutant F129A, sialyl hydrolase, STNA, TcTS, TDE0471, Tr6, trans-sialidase, trans-sialidase 1, TSia, VCNA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.18 exo-alpha-sialidase

Purification

Purification on EC 3.2.1.18 - exo-alpha-sialidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
12287fold, by ammonium precipitation, ultrafiltration, reverse phase, gel filtration and ConA-affinity chromatography, and preparative PAGE
-
310fold by solubilization with Triton CF 54 and ion exchange and fetuin-affinity chromatographic steps
-
54 kDa truncated histidine-tagged variant
677fold, to homogeneity by chromatography on hydroxyapatite, fetuin-agarose, and Mono Q
-
ammonium sulfate precipitation and DEAE cellulose column chromatography
-
amylose resin column chromatography
-
from inoculated chicken egg allantoic fluid, by ultracentrifugation, gel filtration and proteolytic treatment with pronase, soluble 'head' part of the enzyme complex
-
from several strains
-
glutathione-Sepharose 4B column chromatography
His Trap column chromatography
-
HisTrap nickel affinity column chromatography, and Superdex gel filtration
Ni-affinity column chromatography
Ni-NTA agarose column chromatography
Ni-NTA column chromatography and Q-Sepharose column chromatography
Ni-NTA column chromatography and Sephacryl S-100H gel filtration
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography, and Superdex 200 gel filtration
-
Ni2+ affinity column chromatography, and gel filtration
-
nickel-agarose column chromatography and gel filtration
octyl-Sepharose chromatography and Toyopearl HW-55F column chromatography
-
partial
processed, recombinant His-tagged truncation mutant from insect cells by cation exchange and metal affinity chromatography, 1230fold
-
recombinant
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli, 950fold, by ammonium sulfate precipitation, and several chromatographic steps
recombinant enzyme from insect cells by density gradient centrifugation and gel filtration
-
recombinant glutathione-S-transferase fusion protein from Escherichia coli, by a one-step glutathione-affinity chromatography, 40fold
-
recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged protein
-
recombinant His-tagged viral neuraminidase by nickel affinity chromatography, desalting gel filtration, and cation exchange chromatography
recombinant protein
recombinant wild-type and mutant enzymes as MBP-fusion proteins from Escherichia coli strain TB1 by amylose affinity chromatography, the tag is cleaved off by Factor Xa
recombinantly expressed catalytic domain
-
SP Sepharose column chromatography, His-Trap HP column chromatography, and Superdex 200 gel filtration
Strep-Tactin resin column chromatography
subcloned domain of 56500 Da