3.1.7.2: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

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Reaction

Synonyms

(p)ppGpp synthetase/hydrolase, (ppGpp)ase, 3',5'-bis(diphosphate) 3'-pyrophosphate hydrolase, guanosine-3',5'-bis(diphosphate) 3'-diphosphohydrolase, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase, HDDC3, MESH1, metazoan SpoT homolog-1, penta-phosphate guanosine-3'-diphosphohydrolase, penta-phosphate guanosine-3'-pyrophosphohydrolase, ppGpp hydrolase, ppGpp phosphohydrolase, ppGpp-3'-pyrophosphohydrolase, pyrophosphatase, guanosine 3',5'-bis(diphosphate) 3'-, RelSeq

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.7 Diphosphoric-monoester hydrolases

                3.1.7.2 guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

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1	Activating Compound
7120	AA Sequence
1	Application
1	CAS Registry Number
7	Cloned(Commentary)
18	Disease/ Diagnostics
7	General Information
10	Inhibitors
2	kcat/KM [mM/s]
2	KM Value [mM]
6	Localization
19	Metals/Ions
7	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
23	Organism
5	Pathway
12	PDB ID
2	pH Optimum
11	Protein Variants
4	Purification (Commentary)
4	Reaction
1	Reaction Type
23	Reference
4	Specific Activity [micromol/min/mg]
36	Substrates and Products (Substrate)
2	Subunits
20	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Turnover Number [1/s]

Cloned

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Cloned on EC 3.1.7.2 - guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloning of relSeq fragments and the expression is regulated using pBAD vectors

Streptococcus dysgalactiae subsp. equisimilis

-

651696

expression of fragment proteins in Escherichia coli BL21(DE3)

Mycobacterium tuberculosis

-

661218

preparation of mutants with null alleles at both, spoT and relA

Escherichia coli

-

135704

recombinant expression of full-length enzyme in Escherichia coli and in HEK-293T cells

Homo sapiens

-

716313

recombinant expression of full-length enzyme in Escherichia coli and in S2 cells

Drosophila melanogaster

-

716313

spotT gene

Escherichia coli

-

135702

the pET22b expression system is used

Mycobacterium tuberculosis

-

649920