3.1.7.2: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

This is an abbreviated version!
For detailed information about guanosine-3',5'-bis(diphosphate) 3'-diphosphatase, go to the full flat file.

Word Map on EC 3.1.7.2

Reaction

guanosine 3',5'-bis(diphosphate)
+
H2O
=
GDP
+
diphosphate

Synonyms

ppGpp-3'-pyrophosphohydrolase, ppGpp phosphohydrolase, 3',5'-bis(diphosphate) 3'-pyrophosphate hydrolase, pyrophosphatase, guanosine 3',5'-bis(diphosphate) 3'-, guanosine-3',5'-bis(diphosphate) 3'-diphosphohydrolase, penta-phosphate guanosine-3'-pyrophosphohydrolase, penta-phosphate guanosine-3'-diphosphohydrolase, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase, (ppGpp)ase, ppGpp hydrolase, RelSeq, (p)ppGpp synthetase/hydrolase, MESH1, HDDC3, metazoan SpoT homolog-1

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.7 Diphosphoric-monoester hydrolases
                3.1.7.2 guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

Cloned

Cloned on EC 3.1.7.2 - guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of relSeq fragments and the expression is regulated using pBAD vectors
-
expression of fragment proteins in Escherichia coli BL21(DE3)
-
preparation of mutants with null alleles at both, spoT and relA
-
recombinant expression of full-length enzyme in Escherichia coli and in HEK-293T cells
-
recombinant expression of full-length enzyme in Escherichia coli and in S2 cells
-
spotT gene
-
the pET22b expression system is used
-