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3.1.6.13: iduronate-2-sulfatase

This is an abbreviated version!
For detailed information about iduronate-2-sulfatase, go to the full flat file.

Word Map on EC 3.1.6.13

Reaction

L-iduronate-2-sulfate
+
H2O
=
L-iduronate
+
sulfate

Synonyms

2-sulfo-L-iduronate 2-sulfatase, chondroitinsulfatase, elaprase, Hunter corrective factor, I2S, IDS, IDS-Like, IDS-like enzyme, iduronate 2-sulfatase, iduronate 2-sulfate sulfatase-like, iduronate 2-sulfate-like enzyme, iduronate sulfatase, iduronate sulfate sulfatase, iduronate-2-sulfatase, iduronate-2-sulfate sulfatase, iduronate-2-sulphatase, iduronide-2-sulfate sulfatase, idurono-2-sulfatase, idursulfase, L-iduronate 2-sulfate sulfatase, L-idurono sulfate sulfatase, sulfatase, L-idurono-, sulfo-L-iduronate sulfatase, sulfoiduronate sulfohydrolase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.6 Sulfuric-ester hydrolases
                3.1.6.13 iduronate-2-sulfatase

Cloned

Cloned on EC 3.1.6.13 - iduronate-2-sulfatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AAV type 2/5 vector carrying human IDS cDNA under the control of the cytomegalovirus promoter (AAV2/5CMV-hIDS) administered in day 2 idsy/- mouse pups
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chimeric IDS-GFP cloned in a pCS2 vector under the control of the cytomegalovirus promoter. The resulting construct transfected into FU20 primary fibroblasts
development of an expression system for human recombinant IDS in Pichia pastoris and of a detection method for enzyme detection during production and purification processes, which can be used also to measure the enzyme in human fluids, immunoquantification assay using rabbit IgG and chicken IgY, overview
-
expressed in COS-7 cells
expressed in islets, alpha and beta clonal cells
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expression in CHO cell
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expression in CHO cells
-
expression in COS 7 cells
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expression in COS cells and lymphoblastoid cells
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expression in Escherichia coli
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expression in HT-1080 human fibroblasts
expression in Pichia pastoris
expression of identified mutations in COS 7 cells
-
full-length IDS cDNA amplified and cloned into the pCR2.1-TOPO vector
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hIDS c-myc subcloned into pcDNA 3.1/Hygro(-). INS1E cells transiently transfected with the IDS construct
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HIRMAb-IDS fusion protein (IDS cDNA, encoding Ser26-Pro550, minus the 25 amino acid signal peptide, fused to the carboxyl terminus of the heavy chain of a chimeric monoclonal antibody to the human insulin receptor) expressed in COS cells
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Pichia pastoris strain GS115:His- transformed with the integrative plasmid pPIC9-hIDS-Like (Clone IDS28)
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production of the recombinant enzyme by a transfected human clone: Bosc 23 cells and production of the recombinant enzyme by adenoviral transduction of neuronal cells: SK-N-BE
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production of the recombinant enzyme by adenoviral transduction of glial cells: C6
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the expression of enzyme from three promoters in four retroviral vectors are studied
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the recombinant fusion enzyme protein is triaged to the lysosomal compartment of MPS-II fibroblasts, expression in CHO cells as IgG-sulfatase fusion protein with the enzyme fused to a genetically engineered monoclonal antibody against the human insulin receptor, i.e. HIRMAb. The cDNA encoding the human IDS cDNA, minus the sequence encoding the signal peptide, is fused to the carboxyl terminus of the CH3 region of the heavy chain of the chimeric HIRMAb
-