3.1.6.1: arylsulfatase (type I)
This is an abbreviated version!
For detailed information about arylsulfatase (type I), go to the full flat file.
Word Map on EC 3.1.6.1
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3.1.6.1
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lysosomal
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metachromatic
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leukodystrophy
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beta-glucuronidase
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steroid
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urine
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mucopolysaccharidosis
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sulfatide
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leukocyte
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urinary
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asa
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estrone
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glycosaminoglycans
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glucuronide
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demyelination
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cerebroside
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urease
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sulfotransferase
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aromatase
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heparan
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beta-glucosidase
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desulfation
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dehydroepiandrosterone
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dermatan
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infantile
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17beta-hydroxysteroid
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mucopolysaccharide
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pomatia
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ichthyosis
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hexosaminidase
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morquio
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saposins
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iduronate
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17beta-hsds
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galactosylceramide
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alpha-fucosidase
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chondrodysplasia
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hspgs
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synthesis
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medicine
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deconjugation
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analysis
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6-o-sulfate
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tibolone
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alpha-l-iduronidase
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loam
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sanfilippo
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galns
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punctata
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mucolipidosis
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sulfamate
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4-sulfate
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dysostosis
- 3.1.6.1
- lysosomal
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metachromatic
- leukodystrophy
- beta-glucuronidase
- steroid
- urine
- mucopolysaccharidosis
- sulfatide
- leukocyte
- urinary
- asa
- estrone
- glycosaminoglycans
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glucuronide
- demyelination
- cerebroside
- urease
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sulfotransferase
- aromatase
- heparan
- beta-glucosidase
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desulfation
- dehydroepiandrosterone
- dermatan
-
infantile
-
17beta-hydroxysteroid
-
mucopolysaccharide
- pomatia
- ichthyosis
- hexosaminidase
- morquio
-
saposins
-
iduronate
- 17beta-hsds
- galactosylceramide
- alpha-fucosidase
-
chondrodysplasia
-
hspgs
- synthesis
- medicine
-
deconjugation
- analysis
-
6-o-sulfate
- tibolone
- alpha-l-iduronidase
-
loam
-
sanfilippo
- galns
- punctata
- mucolipidosis
- sulfamate
- 4-sulfate
- dysostosis
Reaction
Synonyms
4-methylumbelliferyl sulfatase, ARS, ARS-A, ARS-B, ARSA, ARSB, ARSE, ARSK, ary 423, Aryl-sulfate sulphohydrolase, arylsufatase B, arylsulfatase, arylsulfatase A, arylsulfatase B, arylsulfatase E, arylsulfatase Es-2, arylsulfatase G, arylsulfatase gene, arylsulfatase III, arylsulfatase J, arylsulfatase K, arylsulfatase type VI, arylsulfate sulfohydrolase II, arylsulfohydrolase, arylsulphatase, arylsulphatase A, AS-A, ASG, AstA, AtsA, estrogen sulfatase, KIAA1001, nitrocatechol sulfatase, p-nitrophenyl sulfatase, P70, PAS, phenolsulfatase, phenylsulfatase, sulfatase, sulfatase, aryl-
ECTree
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Application
Application on EC 3.1.6.1 - arylsulfatase (type I)
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analysis
medicine
synthesis
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cytochemical localization of enzyme in cytoplasmic vesicles as a specific detection method for lysosomes
analysis
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distinction of lysosomes and reservosomes by cytochemical localization of enzyme in epimastigote and trypomastigote
analysis
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development of a time-based mass spectrometric assay to analyze substrate conversion and substrate specificity
analysis
enzyme hydrolyzes dehydroepiandrosterone 3-sulfate and epiandrosterone 3-sulfate in a urine matrix. Purified arylsulfatase contains only sulfatase activity allowing for the selective hydrolysis of sulfate esters in the presence of glucuronide conjugates. The enzyme can be employed for short three-step chemoenzymatic synthesis of 5alpha-androstane-3beta,17beta-diol 17-glucuronide from epiandrosterone 3-sulfate
analysis
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high-throughput screen for mutants expressed in Escherichia coli. The alkaline lysis buffer is 1.0 M Tris-HCl at pH 9.0 plus 0.1 % Tween-20 and 2.0 mM 4-aminobenzamidine, mixed with cell suspension at 8:1 to 12:1 ratio for continuous agitation of mixtures in 96-well plates under room temperature. The enzmye tolerates final 0.1 % Tween-20, and activities in lysates are steady from 3 to 9 h and comparable to sonication treatment but better than freezing-thawing
analysis
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enzyme hydrolyzes dehydroepiandrosterone 3-sulfate and epiandrosterone 3-sulfate in a urine matrix. Purified arylsulfatase contains only sulfatase activity allowing for the selective hydrolysis of sulfate esters in the presence of glucuronide conjugates. The enzyme can be employed for short three-step chemoenzymatic synthesis of 5alpha-androstane-3beta,17beta-diol 17-glucuronide from epiandrosterone 3-sulfate
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the enzyme can be accepted as the enzyme component of antitumor antibody-enzyme conjugates for target chemotherapy
medicine
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use of immobilized and column-packed enzyme is an efficient alternative for the cleavage of urinary conjugates in clinical toxicology
medicine
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leukocytes of patients with cerebral palsy show lower enzyme activity as compared to control. Km value for 4-nitrocatechol sulfate in patients' leukocytes is about 0.26 mM, in control 0.21 mM, whereas Vmax value in patients reaches only 58% of healthy control. All investigated patients reveal presence of the most common mutations associated with enzyme pseudodeficiency
medicine
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very low enzymic activity in deciduum of women at 41 weeks of gestation, which reduce to a half at 42 weeks of gestation, while values of sulfatide concentrations increase
medicine
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potential benefits of ARSA brain gene therapy in patients with metachromatic leukodystrophy, MLD
medicine
a one-time injection of human arylsufatase B into injured mouse spinal cord eliminates immunoreactivity for chondroitin sulfates within five days, and up to 9 weeks after injury. After a moderate spinal cord injury, locomotor recovery assessed by the Basso Mouse Scale in arylsulfatase B treated mice improves, compared to the buffer-treated control group, at 6 weeks after injection. After a severe spinal cord injury, mice injected with equivalent units of arylsulfatase B or bacterial chondroitinase ABC improve similarly and both groups achieve significantly more locomotor recovery than the buffer-treated control mice. Serotonin and tyrosine hydroxylase immunoreactive axons are more extensively present in mouse spinal cords treated with arylsulfatase B and chondroitinase ABC, and the immunoreactive axons penetrate further beyond the injury site than in control mice
medicine
assay for the diagnosis of metachromatic leukodystrophy. Determination of arylsulfatase A activity toward the natural substrate, galactosyl-3-sulfate ceramide or sulfatide, is performed using neat sulfatide without chemical modification. The hydrolyzed sulfatide is monitored upon inclusion of the colorimetric reagent Azure A. Within a clinical context, the method definitely discriminates between healthy subject samples and metachromatic leukodystrophy patient samples
medicine
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the enzyme can be accepted as the enzyme component of antitumor antibody-enzyme conjugates for target chemotherapy
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purification method for removing phytoestrogens and related compounds without affecting the enzyme activity. Use of solid phase extraction on a polymeric resin for purification
synthesis
the enzyme could be useful for producing a low sulfated agar and electrophoretic grade agarose
synthesis
immobilization of enzyme on carboxyl functioned magnetic nanoparticles using glutaraldehyde. Immobilization leads to increased tolerance to some metal ions, inhibitors and detergents. Immobilized arylsulfatase maintains 61.7% of its initial desulfuration rates after seven cycles. After the reaction of agar with immobilized arylsulfatase for 90 min at 50°C, 46% of the sulfate in the agar is removed
synthesis
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the enzyme could be useful for producing a low sulfated agar and electrophoretic grade agarose
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