3.1.4.1: phosphodiesterase I
This is an abbreviated version!
For detailed information about phosphodiesterase I, go to the full flat file.
Word Map on EC 3.1.4.1
-
3.1.4.1
-
camp
-
phosphodiesterases
-
calcification
-
arterial
-
topoisomerase
-
rolipram
-
monophosphate
-
camp-specific
-
infancy
-
osteoblast
-
hypophosphatemic
-
rickets
-
camptothecin
-
pseudoxanthoma
-
ectoenzyme
-
topotecan
-
5'-nucleotide
-
elasticum
-
ibmx
-
ankylosis
-
3'-phosphate
-
zaprinast
-
top1-dna
-
camp-pde
-
tcf7l2
-
milrinone
-
qq
-
tissue-nonspecific
-
cgmp-inhibited
-
cgmp-stimulated
-
pyrophosphohydrolase
-
cilomilast
-
cementum
-
3-isobutyl-1-methylxanthine
-
rolipram-sensitive
-
vinpocetine
-
cardiotonic
-
pharmacology
-
medicine
-
synthesis
-
drug development
-
biotechnology
-
analysis
- 3.1.4.1
- camp
- phosphodiesterases
- calcification
- arterial
-
topoisomerase
- rolipram
- monophosphate
-
camp-specific
-
infancy
- osteoblast
-
hypophosphatemic
- rickets
- camptothecin
-
pseudoxanthoma
-
ectoenzyme
- topotecan
-
5'-nucleotide
- elasticum
- ibmx
- ankylosis
- 3'-phosphate
- zaprinast
-
top1-dna
- camp-pde
-
tcf7l2
- milrinone
-
tissue-nonspecific
-
cgmp-inhibited
-
cgmp-stimulated
-
pyrophosphohydrolase
- cilomilast
-
cementum
- 3-isobutyl-1-methylxanthine
-
rolipram-sensitive
- vinpocetine
-
cardiotonic
- pharmacology
- medicine
- synthesis
- drug development
- biotechnology
- analysis
Reaction
Synonyms
5' nucleotide phosphodiesterase/alkaline phosphodiesterase I, 5'-exonuclease, 5'-NPDase, 5'-nucleotide phosphodiesterase, 5'-PDase, 5'-PDE, 5'-phosphodiesterase, 5'NPDE, alkaline phosphodiesterase, Ap3A (Ap4A) hydrolase, autotaxin, B10, calcium-dependent phosphodiesterase 1C, cleavage/polyadenylation specificity factor, CPSF-73, cRNPMase-5'-PDase, cyclic nucleotide phosphodiesterase 1, cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase I, ecto-NPPase, ecto-nucleotide pyrophosphatase/phosphodiesterase 1, ectonucleotide pyrophosphatase phosphodiesterase 1, ectonucleotide pyrophosphatase/phosphodiesterase 1, ectonucleotide pyrophosphate/phosphodiesterase-1, ENPP-1, ENPP1, exonuclease I, exophosphodiesterase, gp130RB13-6, liver alkaline phosphodiesterase 1, liver nucleotide phosphodiesterase, More, NPP, NPP-1, NPP/PDE, NPP1, NPP2, NPP3, nucleotide phosphodiesterase, nucleotide phosphodiesterase/pyrophosphatase, nucleotide pyrophosphatase/alkaline phosphodiesterase I, nucleotide pyrophosphatase/phosphodiesterase 1, nucleotide pyrophosphatase/phosphodiesterase I, nucleotide pyrophosphatase/phosphodiesterase-1, nucleotide pyrophosphatase/phosphodiesterase-2, nucleotide pyrophosphatase/phosphodiesterase-3, orthophosphoric diester phosphohydrolase, PC-1, PDase, PDE, PDE 1A, PDE I, PDE1, PDE1A, PDE1C, PDE4A, PDEase, phosphodiesterase, phosphodiesterase 1, phosphodiesterase 1A, phosphodiesterase 1C, phosphonate monoester hydrolase/phosphodiesterase, phosphoric diester hydrolase, plant exonuclease I, plasma cell antigen 1, plasma cell membrane glycoprotein, plasma cell membrane protein-1, PMH, RNase J1, SMPDL3A, sphingomyelin phosphodiesterase, acid-like 3A, SVPD, Tdp 1, TDP1, tRNase Z, tyrosyl DNA phosphodiesterase 1, tyrosyl-DNA phosphodiesterase 1, tyrosyl-DNA phosphodiesterase I, tyrosyl-DNA-phosphodiesterase I, yfcE
ECTree
3.1.4.1 phosphodiesterase IAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.1.4.1 - phosphodiesterase I
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(5'-GATCTAAAAGACTT-3')-phosphotyrosine + H2O
(5'-GATCTAAAAGACTT-3')-phosphate + L-tyrosine
-
-
-
?
10-hydroxydecyl-oligonucleotide + H2O
?
-
different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site
-
-
?
2-hydroxypropyl-p-nitrophenyl phosphate + H2O
2-hydroxypropyl-p-nitrophenol + phosphate
-
-
-
?
2-naphthyl chloromethylphosphonate + H2O
2-naphthol + chloromethylphosphonic acid
-
-
-
-
?
2-naphthyl phenylphosphonate + H2O
2-naphthol + benzenephosphonic acid
-
-
-
-
?
3'-O-acetyl-5'-O-[hydroxy(4-nitrophenoxy)phosphoryl]thymidine + H2O
p-nitrophenol + 3'-O-acetylthymidine 5'-phosphate
-
-
-
-
?
3'-phosphohistidyl-linked tyrosyl-DNA phosphodiesterase I-DNA complex + H2O
?
tyrosyl-DNA phosphodiesterase I hydrolyzes the 3'phospho-histidyl bond formed between tyrosyl-DNA phosphodiesterase I and DNA
-
-
?
3'-phosphotyrosyl-linked topoisomerase I-DNA complex + H2O
?
tyrosyl-DNA phosphodiesterase I hydrolyzes the 3'phospho-tyrosyl bond formed between DNA topoisomerase I and DNA
-
-
?
3-hydroxy-2(hydroxymethyl)-tetrahydrofuran-oligonucleotide + H2O
?
-
different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site. In the case of tetrahydrofuran, the enzyme generates break with the 5'-tetrahydrofuran and the 3'-phosphate termini
the enzyme generates a non-phosphorylated 5'-THF-terminus by the cleavage of 3-hydroxy-2(hydroxymethyl)-tetrahydrofuran-containing DNA
-
?
4-methylumbelliferyl phenylphosphonate + H2O
4-methylumbelliferone + phenylphosphonic acid
-
-
-
-
?
4-methylumbelliferyl thymidine 5'-phosphate + H2O
4-methylumbelliferone + 5'-TMP
-
-
-
-
?
4-nitrophenyl deoxythymidine 5'-phosphate + H2O
4-nitrophenol + deoxythymidine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phenyl phosphonate + H2O
4-nitrophenol + phenyl phosphonate
-
-
-
-
?
4-nitrophenyl phosphocholine + H2O
4-nitrophenol + phosphocholine
secreted recombinant human SMPDL3A hydrolyzes 4-nitrophenyl phosphorylcholine, a synthetic analogue of the phosphorylcholine headgroup of lipids such as sphingomyelin and phosphatidylcholine
-
-
?
5'-p-nitrophenyladenosine 5'-phosphate + H2O
5'-AMP + p-nitrophenol
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
nucleoside 5'-monophosphate + p-nitrophenol
5'-phosphotyrosyl-linked topoisomerase II-DNA complex + H2O
?
tyrosyl-DNA phosphodiesterase I hydrolyzes the 5'phospho-tyrosyl bond formed between DNA topoisomerase II and DNA
-
-
?
adenylyl-2',5'-adenosine + H2O
?
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
adenylyl-3',5'-cytidine + H2O
adenosine 3'-phosphate + cytidine
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
adenylyl-3',5'-uridine + H2O
?
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
bis(4-nitrophenyl) phosphate + H2O
4-nitrophenyl phosphate + 4-nitrophenol
-
-
-
-
?
bis(4-nitrophenyl)phosphate + H2O
4-nitrophenyl phosphate + 4-nitrophenol
-
-
-
-
?
bis-4-nitrophenyl phosphate + H2O
4-nitrophenol + 4-nitrophenyl phosphate
-
-
-
-
?
bis-ethylene glycolyl-oligonucleotide + H2O
?
-
different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site
-
-
?
cytidylyl-3',5'-adenosine + H2O
?
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
diethenoadenosine polyphosphate + H2O
ethenoadenosine 5'-monophosphate + ethenoadenosine (n-1)5'-polyphosphate
-
-
-
-
?
guanylyl-3',5'-adenosine + H2O
?
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
p-nitrophenyl 5'-thymidine monophosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
?
p-nitrophenyl chloromethylphosphonate + H2O
p-nitrophenol + chloromethylphosphonic acid
-
-
-
-
?
p-nitrophenyl ethyl phosphate + H2O
p-nitrophenol + ethyl hydrogen phosphate
-
-
-
-
?
p-nitrophenyl methylphosphonate + H2O
p-nitrophenol + methylphosphonic acid
-
-
-
-
?
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + phenylphosphonic acid
-
-
-
-
?
P1,P4-bis(5'-uridyl) tetraphosphate + H2O
UMP + uridine 5'-triphosphate
-
-
-
?
thymidine 5'-monophosphate p-nitrophenylester + H2O
? + phosphate
-
-
-
?
thymidine 5'-monophosphate p-nitrophenylester + H2O
p-nitrophenol + TMP
-
-
-
?
uridylyl-3',5'-adenosine + H2O
?
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
4-nitrophenyl uridine 5'-phosphate + H2O
4-nitrophenol + uridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl uridine 5'-phosphate + H2O
4-nitrophenol + uridine 5'-phosphate
-
-
-
-
?
5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
5'-dTMP + p-nitrophenol
-
-
-
-
?
5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
5'-dTMP + p-nitrophenol
-
-
-
-
?
5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
5'-dTMP + p-nitrophenol
-
-
-
-
?
5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
5'-dTMP + p-nitrophenol
-
nucleotide pyrophosphatase/phosphodiesterase I
-
-
?
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
nucleoside 5'-monophosphate + p-nitrophenol
-
-
-
?
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
nucleoside 5'-monophosphate + p-nitrophenol
-
-
-
-
?
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
nucleoside 5'-monophosphate + p-nitrophenol
-
-
-
-
?
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
nucleoside 5'-monophosphate + p-nitrophenol
-
-
-
?
5'-p-nitrophenyluridine 5'-phosphate + H2O
5'-UMP + p-nitrophenol
-
-
-
-
?
5'-p-nitrophenyluridine 5'-phosphate + H2O
5'-UMP + p-nitrophenol
-
70% as fast as 5'-p-nitrophenylthymidine 5'-phosphate
-
-
?
adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
AMP + ATP
-
-
-
-
?
adenylyl-3',5'-adenosine + H2O
adenosine + 5'-AMP
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
ADP + H2O
AMP + phosphate
-
ADPase activity, hydrolysis of ADP by human plasma PDEase acts as an inhibitor of platelet aggregation
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
-
processive two-step mechanism
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
-
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
-
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
-
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenyl phosphate + nitrophenol
-
-
-
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenyl phosphate + nitrophenol
-
-
-
-
?
cAMP + H2O
?
-
incubated with or without 0.2 mM CaCl2, 10 mU calmodulin, and PDE1 inhibitor vinpocetin, 30°C, pH 7.5
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
3',5'-cAMP, slowly
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
PDE1 inhibitory activity assays
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
cyclic AMP: not a substrate
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
phosphodiester bonds
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
phosphodiester bonds
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
PC-1
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
phosphodiester bonds
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
-
slowly
-
?
diadenosine polyphosphate + H2O
AMP + adenosine polyphosphate-1
-
phosphodiesterase can be considered as a catabolic enzyme
-
-
?
diadenosine polyphosphate + H2O
AMP + adenosine polyphosphate-1
-
phosphodiesterase can be considered as a catabolic enzyme
-
?
dinucleoside monophosphates + H2O
mononucleoside 5'-phosphate
-
-
-
?
dinucleoside monophosphates + H2O
mononucleoside 5'-phosphate
-
3'-5' linkage
-
-
?
dinucleoside monophosphates + H2O
mononucleoside 5'-phosphate
-
no specificity toward bases or sugars
-
-
?
dinucleoside monophosphates + H2O
mononucleoside 5'-phosphate
-
2'-5' linkage
-
-
?
dinucleoside monophosphates + H2O
mononucleoside 5'-phosphate
-
3'-5' linkage
-
?
dinucleoside monophosphates + H2O
mononucleoside 5'-phosphate
-
-
-
-
?
dinucleotides + H2O
?
-
3'-hydroxyl-terminated 3'-5'and 2'-5' linkage without specificity toward bases or sugars
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
denatured DNA. Native DNA is resistant
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
denatured DNA. Native DNA is resistant
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
enzyme hydrolyzes single-stranded DNA, no activity with double-stranded DNA
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
enzyme hydrolyzes single-stranded DNA, no activity with double-stranded DNA
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
denatured DNA. Native DNA is resistant
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
native and denatured DNA
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
enzyme hydrolyzes single-stranded DNA, no activity with double-stranded DNA
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
immobilized enzyme: not a substrate
-
-
?
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
heat-denatured: very slowly, native DNA is not hydrolyzed
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
-
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
PC-1
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
-
-
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
p-nitrophenyl 5'-thymidine monophosphate + H2O
p-nitrophenol + 5'-thymidine monophosphate
-
-
-
?
p-nitrophenyl 5'-thymidine monophosphate + H2O
p-nitrophenol + 5'-thymidine monophosphate
-
-
-
-
?
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + benzenephosphonic acid
-
-
-
-
?
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + benzenephosphonic acid
-
-
-
-
?
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + benzenephosphonic acid
-
-
-
-
?
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + benzenephosphonic acid
-
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
PC-1
-
-
?
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
-
-
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
PC-1
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
very slowly
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
-
specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner
-
-
?
RNA + H2O
RNA(n-1) + nucleoside 5'-phosphate
-
highly polymerized RNA: hydrolyzed at medium speed, single-stranded RNA: slowly, tRNA: not a substrate
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RNA + H2O
RNA(n-1) + nucleoside 5'-phosphate
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RNase J1 activity is sensitive to the phosphorylation state of the 5'-end, with severalfold greater activity observed with monophosphorylated or hydroxylated 5'-ends than with triphosphorylated 5'-ends
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RNA + H2O
RNA(n-1) + nucleoside 5'-phosphate
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RNase J1 activity is sensitive to the phosphorylation state of the 5'-end, with severalfold greater activity observed with monophosphorylated or hydroxylated 5'-ends than with triphosphorylated 5'-ends
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RNA + H2O
RNA(n-1) + nucleoside 5'-phosphate
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calf, only single-stranded portion of tRNA from 3'-OH end
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additional information
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AtTDP has Tyr-phosphodiesterase activity for the topoisomerase-DNA complex
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?
additional information
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no activity with dinucleotides bearing 3'-phosphate , 2': 3'-cyclic AMP and 3': 5'-cyclic AMP
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additional information
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enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal
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additional information
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no activity with the cyclic trimer of deoxythymidine 5'-phosphate and adenosine 3',5'-monophosphate
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?
additional information
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new definition for PDE I activity which takes into account its ability to hydrolyze several types of substrates
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additional information
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
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study on substrate specificity and structural requirements using synthetic oligonucleotide derivatives suitable for labeling of nucleic acids
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?
additional information
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the enzyme may be important in nucleotide metabolism on the cell surface
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?
additional information
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E2REL5
substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues
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?
additional information
?
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study on substrate specificity and structural requirements using synthetic oligonucleotide derivatives suitable for labeling of nucleic acids
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?
additional information
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substrate specificity and structural requirements
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?
additional information
?
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substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues
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?
additional information
?
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no activity with cyclic nucleotides such as 2',3'-cyclic AMP, 3',5'-cyclic AMP, or 3',5'-cyclic GMP
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?
additional information
?
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substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues
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?
additional information
?
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
?
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
?
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PC-1, PC-1 may take part in purine uptake for metabolic needs of cells
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?
additional information
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enzyme might function as a modulating factor of platelet aggregation
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?
additional information
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enzyme may be involved in nucleotides metabolism in human plasma
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?
additional information
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PC-1 plays a major role in bone and cartilage metabolism by producing diphosphate
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?
additional information
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tyrosyl-DNA phosphodiesterase 1 catalyses the hydrolysis of phosphodiester linkages between a DNA 3' phosphate and a tyrosine residue as well as a variety of other DNA 3 substituents, the enzyme shows affinity for the substrate increased as the DNA length
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?
additional information
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Tdp1 activity on DNA substrate with 3'-dRP moiety in the single strand break. The 15-mer with 3'-dRP is generated by incubating 10 nM AP-DNA with EndoIII, site cleavage activity of tyrosyl-DNA phosphodiesterase 1, overview
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?
additional information
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the active site H263 is covalently bound through a phosphoamide bond to the 3' end of DNA moiety of the substrate
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?
additional information
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SMPDL3A is a functional metallophosphoesterase, and despite having no detectable activity towards sphingomyelin, possesses a nucleotide phosphodiesterase activity, particularly strongly against nucleotide triphosphates
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?
additional information
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SMPDL3A is a functional metallophosphoesterase, and despite having no detectable activity towards sphingomyelin, possesses a nucleotide phosphodiesterase activity, particularly strongly against nucleotide triphosphates
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?
additional information
?
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substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues, substrates vary in size and complexity
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?
additional information
?
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the enzyme catalyzes the hydrolysis of 3' phosphotyrosyl linkers and other 3'-end blocking lesions. When Top1 nicks double-stranded DNA, a covalent cleavage complex is formed that can be repaired by the enzyme, the mechanism involves the following steps: first, as Lys265 and Lys495 residues in the catalytic site coordinate the oxygen atoms of the phosphate group, His263 attacks the phosphorus atom linked to the oxygen of the Top1 catalytic residue, Tyr723, at the 3' end of DNA. Second, the TDP1-DNA covalent intermediate formed is hydrolyzed by a His493-activated water molecule, leading to the generation of a DNA product with a 3' phosphate
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?
additional information
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the enzyme cleaves the apurinic/apyrimidinic site in DNA by hydrolysis of the phosphodiester bond between the substituent and 5' adjacent phosphate. The product of enzyme cleavage in the case of the apurinic/apyrimidinic site is unstable and is hydrolyzed with the formation of 3'- and 5'-margin phosphates. The following repair demands the ordered action of polynucleotide kinase phosphorylase, with XRCC1, DNA polymerase beta, and DNA ligase, mechanism, overview
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additional information
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tyrosyl-DNA phosphodiesterase 1 catalyzes the hydrolysis of the phosphodiester linkage between the DNA 3'-phosphate and a tyrosine residue as well as a variety of other DNA 30 damaged termini. The enzyme can liberate the 3'-DNA phosphate termini from apurinic/apyrimidinic sites. The enzyme is more active in the cleavage of the apurinic/apyrimidinic sites inside bubble-DNA structure in comparison to ssDNA containing apurinic/apyrimidinic site, it hydrolyzes apurinic/apyrimidinic sites opposite to bulky fluorescein adduct faster than apurinic/apyrimidinic sites located in dsDNA, specificity of the apurinic/apyrimidinic site cleavage activity
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additional information
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tyrosyl-DNA phosphodiesterase I specifically catalyzes the hydrolysis of the phosphodiester bond between the catalytic Tyr723 of Top1 and DNA-3'-phosphate, then polynucleotide kinase phosphatase hydrolyzes the resulting 3'-phosphate end and catalyzes the phosphorylation of the 5'-hydroxyl end of the broken DNA strand. This results in a broken DNA strand with termini consisting of a 5'-phosphate and 3'-hydroxyl for DNA repair. DNA polymerase beta replaces the missing DNA segment, and finally DNA ligase III reseals the broken DNA
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?
additional information
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efficiency of apurinic/apyrimidinic site hydrolysis catalyzed by the enzyme decreases in the order: dsAP-DNA/bubble > ssAP-DNA > dsAP-DNA/Flu >> dsAP-DNA, the enzyme shows preference for 3'-substituent located on the termini of DNA compared to nicks
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additional information
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isozymes NPP1 and NPP3 can hydrolyze ATP, as well as inhibitor molecule 4, they also show very low activity with inhibitors 1 and 2, 1-4% compared to the activit with ATP
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additional information
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recombinant human enzyme processes 5'-phosphotyrosyl DNA ends when they mimic the 5'-overhangs of topoisomerase II-DNA cleavage complexes and 3'-deoxyribose phosphates generated from hydrolysis of abasic sites
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?
additional information
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SMPDL3A is not an acid sphingomyelinase, but unexpectedly is active against nucleotide diphosphate and triphosphate substrates at acidic and neutral pH
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additional information
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SMPDL3A is not an acid sphingomyelinase, but unexpectedly is active against nucleotide diphosphate and triphosphate substrates at acidic and neutral pH
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additional information
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substrate is 5'-[32P]-labeled single-stranded DNA oligonucleotide containing a 3'-phosphotyrosine (N14Y)
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additional information
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substrate is a 5'-[32P]-labeled single-stranded DNA oligonucleotide containing a 3' phosphotyrosine (N14Y)
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additional information
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the enzyme is able to act as a limited 3'-exonuclease by removing a single RNA or DNA nucleotide from the 3'-end of the DNA, but only if it bears a 3'-hydroxyl-group rather than a 3'-phosphoryl-group. The latter restriction prevents the enzyme from acting as a classic endonuclease to degrade the products of its own catalysis
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additional information
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the enzyme is also able to effectively cleave non-nucleotide insertions in DNA, decanediol and diethyleneglycol moieties by the same mechanism as in the case of tetrahydrofuran cleavage, it catalyzes the hydrolysis of decanediol- and bis-ethylene glycol-containing DNA even more effectively than tetrahydrofuran-containing DNA particularly in the case of decanediol-containing DNA. Repair of 5'-tetrahydrofuran and other apurinic/apyrimidinic site analogues can be processed by the long-patch base excision repair pathway, overview.The efficiency of enzyme-catalyzed hydrolysis of apurinic/apyrimidinic-site analogues correlates with the DNA helix distortion induced by the substituent
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?
additional information
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the enzyme cleaves the 3'-diester bond between tyrosine and DNA as well as removes other lesions, producing a 3'-phosphate that can be removed by polynucleotide kinase/phosphatase
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?
additional information
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the enzyme hydrolyzes the phosphodiester bond between a catalytic tyrosine Tyr723 (human) of topoisomerase 1 and DNA 3'-phosphate
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?
additional information
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the physiological function of PDE in plant cell is presumably the degradation of nucleic acids
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?
additional information
?
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no activity with dinucleotides bearing 3'-phosphate , 2': 3'-cyclic AMP and 3': 5'-cyclic AMP
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?
additional information
?
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
?
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no activity with dinucleotides bearing 3'-phosphate , 2': 3'-cyclic AMP and 3': 5'-cyclic AMP
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?
additional information
?
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
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product of the reaction is a 3'-phosphate and not a 3'-hydroxyl
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additional information
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product of the reaction is a 3'-phosphate and not a 3'-hydroxyl
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additional information
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implication of enzyme in intra- and extracellular processes, overview
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?
additional information
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PC-1, PC-1 may take part in purine uptake for metabolic needs of cells
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?
additional information
?
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PC-1, PC-1 may take part in purine uptake for metabolic needs of cells
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?
additional information
?
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PC-1, nucleotide pyrophosphatase/alkaline phosphodiesterase I, may have a role in the regulation of N-linked glycosylation, it may regulate the availability of nucleotide sugar precusors at the site of oligosaccharide synthesis
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?
additional information
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substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues
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?
additional information
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PC-1, nucleotide pyrophosphatase/alkaline phosphodiesterase I, may have a role in the regulation of N-linked glycosylation, it may regulate the availability of nucleotide sugar precusors at the site of oligosaccharide synthesis
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?
additional information
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NPP1 exhibits hydrolytic activity toward ADP-glucose and plastid-targeting ability
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?
additional information
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NPP1 exhibits hydrolytic activity toward ADP-glucose and plastid-targeting ability
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?
additional information
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unlike isozyme NPP1, isozyme NPP3 exhibits no hydrolytic activity toward ADP-glucose and no plastid-targeting ability
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additional information
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unlike isozyme NPP1, isozyme NPP3 exhibits no hydrolytic activity toward ADP-glucose and no plastid-targeting ability
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additional information
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no activity with dinucleotides bearing 3'-phosphate
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?
additional information
?
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enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal
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?
additional information
?
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
?
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phosphodiesterase activity results in the cleavage of nucleotides from nucleic acids and thus may be involved in catabolism of extracellular DNA. Essential function may be to hydrolyze polyribo- and polydeoxyribonucleotides. The enzyme may be important in cell differentiation during carcinogenesis
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additional information
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PC-1, plasma-membrane-associated enzyme with nucleotide pyrophosphatase/phosphodiesterase I activity is probably involved in the clearance of extracellular nucleotides such as ATP and diadenosine polyphosphates
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additional information
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isoform NPP1 is involved in cleavage of extracellular diadenosine polyphosphates in brain
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additional information
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substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues
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?
additional information
?
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PMH hydrolyses phosphonate monoesters and phosphate diesters with similar efficiency
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?
additional information
?
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substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues, substrates vary in size and complexity
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?
additional information
?
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the enzyme hydrolyzes the covalent bond between the tyrosyl residue of topoisomerase 1 and the 3'-phosphate group in DNA. The enzyme is able to initiate the cleavage of internal apurinic/apyrimidinic sites or 3-hydroxy-2-hydroxymethyl tetrahydrofuran in DNA and its subsequent repair
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additional information
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the enzyme hydrolyzes the covalent bond between the tyrosyl residue of topoisomerase 1 and the 3'-phosphate group in DNA. The enzyme is able to initiate the cleavage of internal apurinic/apyrimidinic sites or 3-hydroxy-2-hydroxymethyl tetrahydrofuran in DNA and its subsequent repair
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?
additional information
?
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substrates are protein-DNA adducts, such as camptothecin stabilized Topo1-DNA adducts, and modified nucleotides, including oxidized nucleotides and chain terminating nucleoside analogues, substrates vary in size and complexity
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?
additional information
?
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no activity with dinucleotides bearing 3'-phosphate
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?
additional information
?
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enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal
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?
additional information
?
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
additional information
?
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no activity with p-nitrophenylthymidine 3'-phosphate
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?
