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3.1.30.1: Aspergillus nuclease S1

This is an abbreviated version!
For detailed information about Aspergillus nuclease S1, go to the full flat file.

Word Map on EC 3.1.30.1

Reaction

endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products =

Synonyms

Alteromonas BAL 31 nuclease, Aspergillus oryzae S1 nuclease, Bacillus subtilis deoxyribonuclease, Bh1, CEL I, Deoxyribonuclease P1, deoxyribonuclease S1, DNase, EC 3.1.4.21, ENDO3, endonuclease 3, Endonuclease P1, Endonuclease S1, endonuclease S1 (Aspergillus), KB endonuclease, M1 nuclease, mung bean endonuclease, mung bean nuclease, mung bean nuclease I, NcNase, Neurospora crassa endonuclease, Neurospora crassa nuclease, Neurospora crassa single-strand specific endonuclease, nuclease I, nuclease S1, P1 nuclease, P4, Phage T4 endonuclease IV, Physarum polycephalum nuclease, Pisum sativum endonuclease, plant S1-like nuclease, resolvase T7, Rsn, rye germ nuclease, S1, S1 nuclease, S1-like nuclease, S1-nuclease, S1-type nuclease, SC nuclease, Schizosaccharomyces pombe endonuclease, single strand preferring nuclease, single-strand endodeoxyribonuclease, single-strand-preferring nuclease (SSP nuclease), single-strand-specific DNase, single-strand-specific endodeoxyribonuclease, single-strand-specific nuclease, single-strand-specific S1 endonuclease, single-stranded DNA specific endonuclease, single-stranded-nucleate endonuclease, SSPN, T7 endo I, T7 endonuclease, T7 endonuclease I, Ustilago maydis nuclease, wheat seedling nuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.30 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters
                3.1.30.1 Aspergillus nuclease S1

Temperature Stability

Temperature Stability on EC 3.1.30.1 - Aspergillus nuclease S1

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 70
50
-
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min
55 - 65
-
no inactivation by heating at 55°C, partial inactivation at 65°C, importance of high content of hydrophobic amino acids and zinc atoms for thermostability postulated
60
-
after 90 min incubation time 90% of activity towards 3'-AMP and 60% of activity towards DNA lost
60 - 67
-
stable below 60°C, 50% inactivation after heating at 67°C for 15 min, pH 6.0
60 - 70
-
stable to heating at pH 5.0 in the presence of both Zn2+ and sulfhydryl compounds
60 - 80
-
at 70°C complete loss of activity of the soluble enzyme, 40% of maximal activity of the immobilized enzyme at this temperature, 20% at 80°C
65
-
no inactivation of ribonuclease and deoxyribonuclease activity by heating at this temperature in the presence of substrate