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3.1.30.1: Aspergillus nuclease S1

This is an abbreviated version!
For detailed information about Aspergillus nuclease S1, go to the full flat file.

Word Map on EC 3.1.30.1

Reaction

endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products =

Synonyms

Alteromonas BAL 31 nuclease, Aspergillus oryzae S1 nuclease, Bacillus subtilis deoxyribonuclease, Bh1, CEL I, Deoxyribonuclease P1, deoxyribonuclease S1, DNase, EC 3.1.4.21, ENDO3, endonuclease 3, Endonuclease P1, Endonuclease S1, endonuclease S1 (Aspergillus), KB endonuclease, M1 nuclease, mung bean endonuclease, mung bean nuclease, mung bean nuclease I, NcNase, Neurospora crassa endonuclease, Neurospora crassa nuclease, Neurospora crassa single-strand specific endonuclease, nuclease I, nuclease S1, P1 nuclease, P4, Phage T4 endonuclease IV, Physarum polycephalum nuclease, Pisum sativum endonuclease, plant S1-like nuclease, resolvase T7, Rsn, rye germ nuclease, S1, S1 nuclease, S1-like nuclease, S1-nuclease, S1-type nuclease, SC nuclease, Schizosaccharomyces pombe endonuclease, single strand preferring nuclease, single-strand endodeoxyribonuclease, single-strand-preferring nuclease (SSP nuclease), single-strand-specific DNase, single-strand-specific endodeoxyribonuclease, single-strand-specific nuclease, single-strand-specific S1 endonuclease, single-stranded DNA specific endonuclease, single-stranded-nucleate endonuclease, SSPN, T7 endo I, T7 endonuclease, T7 endonuclease I, Ustilago maydis nuclease, wheat seedling nuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.30 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters
                3.1.30.1 Aspergillus nuclease S1

Substrates Products

Substrates Products on EC 3.1.30.1 - Aspergillus nuclease S1

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-AMP + H2O
adenosine + phosphate
show the reaction diagram
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
3'-dAMP + H2O
3'-deoxyadenosine + phosphate
show the reaction diagram
3'-dCMP + H2O
deoxycytosine + phosphate
show the reaction diagram
3'-dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
3'-dTMP + H2O
deoxythymidine + phosphate
show the reaction diagram
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
adenosine 3',5'-bisphosphate + H2O
5'-AMP + phosphate
show the reaction diagram
adenosine 3'-diphosphate 5'-phosphate
5'-AMP + diphosphate
show the reaction diagram
-
-
-
?
ApA + H2O
adenosine + AMP
show the reaction diagram
-
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
almost no hydrolysis
-
?
denatured DNA + H2O
?
show the reaction diagram
deoxythymidine 3',5'-bisphosphate
5'-dTMP + phosphate
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
DNA + H2O
?
show the reaction diagram
-
-
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
duplex deoxyadenosine-deoxythymine homopolymer + H2O
5'-dAMP + 5'-TMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
heteroduplex DNA + H2O
nicked DNA
show the reaction diagram
platinum-DNA complexes + H2O
5'-phosphomononucleotides + platinum
show the reaction diagram
-
native, denaturated and renaturated platinum-DNA complexes, use of several platinum compounds like diamminedichloroplatinum(II)
-
?
poly(U) + H2O
?
show the reaction diagram
-
preferred substrate
-
-
?
polyA + H2O
fragments of polyA
show the reaction diagram
-
-
-
-
?
polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
show the reaction diagram
polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
show the reaction diagram
polydeoxyadenylic acid + H2O
5'-dAMP + 5'-phosphooligonucleotides
show the reaction diagram
polydeoxycytosylic acid + H2O
5'-dCMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only little degradation
-
?
polydeoxyguanylic acid + H2O
5'-dGMP + 5'-phosphooligonucleotides
show the reaction diagram
polydeoxythymidylic acid + H2O
5'-dTMP + 5'-phosphooligonucleotides
show the reaction diagram
polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
show the reaction diagram
polyinosinic acid + H2O
5'-IMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyU + H2O
fragments of polyU
show the reaction diagram
-
-
-
-
?
polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
show the reaction diagram
RNA + H2O
5'-mononucleotides
show the reaction diagram
-
AMP-preferential endoexonuclease
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
RNA + H2O
?
show the reaction diagram
-
-
-
-
?
RNA + H2O
fragments of RNA
show the reaction diagram
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
tRNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
additional information
?
-