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3.1.30.1: Aspergillus nuclease S1

This is an abbreviated version!
For detailed information about Aspergillus nuclease S1, go to the full flat file.

Word Map on EC 3.1.30.1

Reaction

endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products =

Synonyms

Alteromonas BAL 31 nuclease, Aspergillus oryzae S1 nuclease, Bacillus subtilis deoxyribonuclease, Bh1, CEL I, Deoxyribonuclease P1, deoxyribonuclease S1, DNase, EC 3.1.4.21, ENDO3, endonuclease 3, Endonuclease P1, Endonuclease S1, endonuclease S1 (Aspergillus), KB endonuclease, M1 nuclease, mung bean endonuclease, mung bean nuclease, mung bean nuclease I, NcNase, Neurospora crassa endonuclease, Neurospora crassa nuclease, Neurospora crassa single-strand specific endonuclease, nuclease I, nuclease S1, P1 nuclease, P4, Phage T4 endonuclease IV, Physarum polycephalum nuclease, Pisum sativum endonuclease, plant S1-like nuclease, resolvase T7, Rsn, rye germ nuclease, S1, S1 nuclease, S1-like nuclease, S1-nuclease, S1-type nuclease, SC nuclease, Schizosaccharomyces pombe endonuclease, single strand preferring nuclease, single-strand endodeoxyribonuclease, single-strand-preferring nuclease (SSP nuclease), single-strand-specific DNase, single-strand-specific endodeoxyribonuclease, single-strand-specific nuclease, single-strand-specific S1 endonuclease, single-stranded DNA specific endonuclease, single-stranded-nucleate endonuclease, SSPN, T7 endo I, T7 endonuclease, T7 endonuclease I, Ustilago maydis nuclease, wheat seedling nuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.30 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters
                3.1.30.1 Aspergillus nuclease S1

Engineering

Engineering on EC 3.1.30.1 - Aspergillus nuclease S1

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D55E
-
no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+
D74A
-
lower binding affinity for DNA as wild-type, approx. 15% of wild-type DNA cleavage rate
D74N
-
lower binding affinity for DNA as wild-type, approx. 30% of wild-type DNA cleavage rate
E20D
-
limited cleavage of x strand 5'-labeled junction 3 in presence of Mg2+
E35K
-
lower binding affinity for DNA as wild-type, slightly lower rate of DNA cleavage
E65D
-
no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+, limited activity is restored in presence of Mn2+
additional information
-
mutation in beta-bridge center that separates the catalytic domains, 20-50 times lower nonspecific nuclease activity in Mg2+ buffer