usage of CRISPR/Cas9-mediated genome editing to generate a loss-of-function bpnt-1 allele, qd303, in which a deletion/insertion leads to a frameshift at amino acid 20 followed by a premature stop codon. bpnt-1 mRNA is almost entirely absent in the bpnt-1(qd303) mutant, demonstrating that the bpnt-1 expression pattern is specific. Animals carrying bpnt-1(qd303), or the W294X nonsense mutation bpnt-1(gk469190), fail to express daf-7 in the ASJ neurons when exposed to Pseudomonas aeruginosa. Notably, the constitutive expression of daf-7 in the ASI neuron pair is unaffected by loss of bpnt-1. BPNT-1 is required for the expression of daf-7 specifically in the ASJ neurons
usage of CRISPR/Cas9-mediated genome editing to generate a loss-of-function bpnt-1 allele, qd303, in which a deletion/insertion leads to a frameshift at amino acid 20 followed by a premature stop codon. bpnt-1 mRNA is almost entirely absent in the bpnt-1(qd303) mutant, demonstrating that the bpnt-1 expression pattern is specific. Animals carrying bpnt-1(qd303), or the W294X nonsense mutation bpnt-1(gk469190), fail to express daf-7 in the ASJ neurons when exposed to Pseudomonas aeruginosa. Notably, the constitutive expression of daf-7 in the ASI neuron pair is unaffected by loss of bpnt-1. BPNT-1 is required for the expression of daf-7 specifically in the ASJ neurons