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3.1.3.5: 5'-nucleotidase

This is an abbreviated version!
For detailed information about 5'-nucleotidase, go to the full flat file.

Word Map on EC 3.1.3.5

Reaction

A 5'-ribonucleotide
+
H2O
=
a ribonucleoside
 +
phosphate

Synonyms

5'-adenylic phosphatase, 5'-AMP nucleotidase, 5'-AMPase, 5'-ectonucleotidase, 5'-mononucleotidase, 5'-NT, 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4, 5'-nucleotidase, 5'-nucleotidase A, 5'-nucleotidase I, 5'-nucleotidase II, 5'Nase, 5'nucleotidase, 5nA, 5NT, adenosine 5'-monophosphatase, adenosine 5'-phosphatase, adenosine monophosphatase, AMP phosphatase, AMP phosphohydrolase, AMP-selective 5'-nucleotidase, AMPase, c-N-I, c-N-II, Cant1, CD73, CD73 antigen, CD73/ecto-5'-nucleotidase, class C acid phosphatase, cN-I, cN-IA, cN-II, cN-III, cNI, cNII, cNIIIB nucleotidase, cytoplasmic 5'-nucleotidase II, cytoplasmic 5'-nucleotidase II (IMP-selective or GMP/IMP-selective), cytoplasmic 5'-nucleotidase III, cytoplasmic 5'-nucleotidase-I (AMP-selective), cytosolic 5'-nucleotidase, cytosolic 5'-nucleotidase II, cytosolic 5'-nucleotidase/phosphotransferase, E-5'-Nu, e-5NT, e-N, e5'-NT, e5'NT, ecto 5'-NT, ecto 5'-nucleotidase, ecto-5' nucleotidase, ecto-5'-NT, ecto-5'-NT/CD73, ecto-5'-nucleotidase, ecto-5'-nucleotidase/CD73, ecto-5'nucleotidase, ecto-5-NT, ecto-5-nucleotidase, ecto-nucleotidase, ectonuceotidase, ectonucleotidase, ectonucleotidase CD73, eN, eNs, eNT, GA-AMPase, high Km 5'-nucleotidase, high-Km 5'-NT, hppA gene product, IMP 5'-nucleotidase, IMP-GMP 5'-nucleotidase, IMP-GMP specific nucleotidase, IMP-specific, high Km 5'-nucleotidase, IMP/GMP selective 5'-NT, Isn1, membrane-bound 5'-nucleotidase, More, NMN 5'-nucleotidase, NT5C3A, NT5E, P5'N-1, Phm8, Pho5, PM-AMPase, PN-1, PN-I, purine 5'-NT, purine 5'-nucleotidase, pyrimidine 5'-nucleotidase, Sdt1, snake venom 5'-nucleotidase, soluble calcium-activated nucleotidase 1, thymidine monophosphate nucleotidase, type II cytosolic 5'-nucleotidase, UMPase, UMPH-1, uridine 5'-nucleotidase, uridine monophosphate hydrolase-1, UshA, XF_2089, YutF

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.5 5'-nucleotidase

Molecular Weight

Molecular Weight on EC 3.1.3.5 - 5'-nucleotidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
107000
-
gel filtration
119000
-
enzyme N-II, gel filtration
120000
gel filtration
130000
131000
132000
-
eNT from lung, SDS-PAGE
135000
-
enzyme from glioblastoma cells, gel filtration in presence of 0.6% sodium deoxycholate
140000
140000 - 150000
-
gel filtration, sucrose density gradient centrifugation
144000
-
eNT from skeletal muscle, kidney, heart and liver, SDS-PAGE
147000
-
heart, membrane-bound, SDS-PAGE after cross-linkage with dimethylsuberimidate
148000
-
1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE
150000
180000
-
gel filtration
182000
-
liver cytosol, absence of ATP, allosteric protein, binding of adenine nucleotides induces a more compact and stable conformation, calculation from Stokes radius and partial specific volume
185000
-
gel filtration
191000
-
liver cytosol, SDS-PAGE after cross-linkage with dimethylsuberimidate
195000
-
gel filtration
197000
-
liver cytosol, presence of 3 mM ATP, allosteric protein, binding of adenine nucleotides induces a more compact and stable conformation, calculation from Stokes radius and partial specific volume
205000
-
sucrose density gradient centrifugation
210000
-
gel filtration
230000
-
brain cytoplasm, gel filtration
24300
x * 24300, SDS-PAGE
24500
-
1 * 24500 + 1 * 25500, SDS-PAGE
250000
-
cytosolic enzyme, gel filtration
25500
-
1 * 24500 + 1 * 25500, SDS-PAGE
260000
-
liver microsomes, gel filtration in presence of detergents
26300
-
x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE
265000
-
membrane-bound enzyme, gel filtration
268000
270000
-
gel filtration
286000
-
enzyme from synaptic plasma membrane, gel filtration
33000
-
3 * 33000, SDS-PAGE
35900
2 * 35900, SDS-PAGE
43000
-
x * 43000, SDS-PAGE
47700
x * 47700, calculation from nucleotide sequence
48500
-
gel filtration
49000
-
gel filtration
51000
52000
-
enzyme N-I, gel filtration
52400
-
x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE
53000
-
4 * 53000, cytoplasmic enzyme, SDS-PAGE
53700
-
x * 53700, enzyme from Golgi apparatus, SDS-PAGE
54300
-
4 * 54300, brain cytoplasm, SDS-PAGE
55000
-
x * 55000, plasma membrane enzyme, SDS-PAGE
56000
-
x * 56000, membrane-bound enzyme, deglycosylated, SDS-PAGE
57000
57300
-
4 * 57300, calculated from amino acid sequence
58000
-
x * 67000, glycosylated enzyme form, x * 58000, deglycosylated enzyme form, SDS-PAGE
60000
-
x * 60000, SDS-PAGE
62000
-
2 * 62000, SDS-PAGE
63000
-
x * 63000, SDS-PAGE
64432
x * 64432, calculated
64480
x * 64480, calculated
65000
66000
66200
67000
68000
70000
71000
72000
73000
-
x * 73000, membrane-bound enzyme, SDS-PAGE under reducing conditions
74000
74300
gel filtration
78000
-
gel filtration
85000
-
x * 92000, glycosylated enzyme form, x * 85000 deglycosylated enzyme form, SDS-PAGE
92000
-
x * 92000, glycosylated enzyme form, x * 85000 deglycosylated enzyme form, SDS-PAGE
94000
-
1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE