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3.1.3.5: 5'-nucleotidase

This is an abbreviated version!
For detailed information about 5'-nucleotidase, go to the full flat file.

Word Map on EC 3.1.3.5

Reaction

A 5'-ribonucleotide
+
H2O
=
a ribonucleoside
+
phosphate

Synonyms

5'-adenylic phosphatase, 5'-AMP nucleotidase, 5'-AMPase, 5'-ectonucleotidase, 5'-mononucleotidase, 5'-NT, 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4, 5'-nucleotidase, 5'-nucleotidase A, 5'-nucleotidase I, 5'-nucleotidase II, 5'Nase, 5'nucleotidase, 5nA, 5NT, adenosine 5'-monophosphatase, adenosine 5'-phosphatase, adenosine monophosphatase, AMP phosphatase, AMP phosphohydrolase, AMP-selective 5'-nucleotidase, AMPase, c-N-I, c-N-II, Cant1, CD73, CD73 antigen, CD73/ecto-5'-nucleotidase, class C acid phosphatase, cN-I, cN-IA, cN-II, cN-III, cNI, cNII, cNIIIB nucleotidase, cytoplasmic 5'-nucleotidase II, cytoplasmic 5'-nucleotidase II (IMP-selective or GMP/IMP-selective), cytoplasmic 5'-nucleotidase III, cytoplasmic 5'-nucleotidase-I (AMP-selective), cytosolic 5'-nucleotidase, cytosolic 5'-nucleotidase II, cytosolic 5'-nucleotidase/phosphotransferase, E-5'-Nu, e-5NT, e-N, e5'-NT, e5'NT, ecto 5'-NT, ecto 5'-nucleotidase, ecto-5' nucleotidase, ecto-5'-NT, ecto-5'-NT/CD73, ecto-5'-nucleotidase, ecto-5'-nucleotidase/CD73, ecto-5'nucleotidase, ecto-5-NT, ecto-5-nucleotidase, ecto-nucleotidase, ectonuceotidase, ectonucleotidase, ectonucleotidase CD73, eN, eNs, eNT, GA-AMPase, high Km 5'-nucleotidase, high-Km 5'-NT, hppA gene product, IMP 5'-nucleotidase, IMP-GMP 5'-nucleotidase, IMP-GMP specific nucleotidase, IMP-specific, high Km 5'-nucleotidase, IMP/GMP selective 5'-NT, Isn1, membrane-bound 5'-nucleotidase, More, NMN 5'-nucleotidase, NT5C3A, NT5E, P5'N-1, Phm8, Pho5, PM-AMPase, PN-1, PN-I, purine 5'-NT, purine 5'-nucleotidase, pyrimidine 5'-nucleotidase, Sdt1, snake venom 5'-nucleotidase, soluble calcium-activated nucleotidase 1, thymidine monophosphate nucleotidase, type II cytosolic 5'-nucleotidase, UMPase, UMPH-1, uridine 5'-nucleotidase, uridine monophosphate hydrolase-1, UshA, XF_2089, YutF

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.5 5'-nucleotidase

Cloned

Cloned on EC 3.1.3.5 - 5'-nucleotidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytically active recombinant soluble glutathione-S transferase/ecto-5'-NT fusion protein is expressed in insect cells using the baculovirus system
-
cloned into pET21b and overexpressed in Escherichia coli
expressed in COS-7 cells
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) RIL cells
-
expressed in Escherichia coli Rosetta2(DE) cells
-
expressed in Saccharomyces cerevisiae strain Y187 and Escherichia coli BL21 (DE3) cells
expressed in Sf9 insect cells
expression in Escherichia coli, recombinant enzyme is localized in periplasm of Escherichia coli
-
expression in Sf9 cells
-
expression in Sf9 insect cells
expression of His6-tagged wild-type and mutant enzymes
expression of mutant D52N in Escherichia coli strain BL21(DE)-R3-pRARE2
gene NT5E at 6q14-q21 encodes CD73
-
glutathione-S-transferase fusion protein expressed in insect cells using a baculovirus system
-
glutathione-S-transferase fusion protein expressed in Sf9 insect cells
-
overexpression of cN-IA in neonatal rat cardiomyocytes using an adenovirus. The overexpressed enzyme can be sufficiently active in cardiomyocytes to generate physiologically effective concentrations of adenosine at its receptors
-
recombinant expression in COS-7 cells
-