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3.1.3.3: phosphoserine phosphatase

This is an abbreviated version!
For detailed information about phosphoserine phosphatase, go to the full flat file.

Word Map on EC 3.1.3.3

Reaction

O-phospho-L(or D)-serine
+
H2O
=
L(or D)-serine
 +
phosphate

Synonyms

3-phosphoserine phosphatase, BSU04700, BSU04740, BSU34110, CTD phosphatase fcp1, HPSP, iPSP, iPSP1, iPSP2, L-phosphoserine phosphatase, metal-independent phosphoserine phosphatase, metal-independent PSP, More, O-phosphoserine phosphohydrolase, phosphatase, phosphoserine, phosphoserine phosphatase, phosphoserine phosphatase RsbP, phosphoserine phosphatase RsbU, phosphoserine phosphatase RsbX, phosphoserine:homoserine phosphotransferase, PP1, PSP, PSP1, pspA, PSPase, pspB, PSPH, RsbP, RsbU, RsbX, SerB, SerB PG0653, SerB2, SerB653, serine/threonine protein phosphatase, sigma-B negative effector, StP, THrH, TON_1161, yvfP

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.3 phosphoserine phosphatase

Engineering

Engineering on EC 3.1.3.3 - phosphoserine phosphatase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E29D
-
less than 5% of the activity of wild-type enzyme with L-phosphoserine as substrate
E29Q
-
inactive mutant enzyme
N133A
-
about 25% of the activity of wild-type enzyme with L-phosphoserine as substrate
N133D
-
about 130% of the activity of wild-type enzyme with L-phosphoserine as substrate
R202A
-
inactive mutant enzyme
R202K
-
less than 5% of the activity of wild-type enzyme with L-phosphoserine as substrate
R65A
-
inactive mutant enzyme
R65K
-
inactive mutant enzyme
S23A
-
about 50% of the activity of wild-type enzyme with L-phosphoserine as substrate
S23T
-
about 20% of the activity of wild-type enzyme with L-phosphoserine as substrate
T182S
-
about 5% of the activity of wild-type enzyme with L-phosphoserine as substrate
T182V
-
about 75% of the activity of wild-type enzyme with L-phosphoserine as substrate
delI211
50fold decrease in catalytic efficiency
delVal2-I211
400fold decrease in catalytic efficiency
H85A
almost complete loss of activity
H9A
almost complete loss of activity
delI211
-
50fold decrease in catalytic efficiency
-
delVal2-I211
-
400fold decrease in catalytic efficiency
-
H85A
-
almost complete loss of activity
-
H9A
-
almost complete loss of activity
-
D15A/E33A
the mutant shows reduced activity compared to the wild type enzyme
D185G
complete loss of activity
D341G
about 10% residual activity, no change in secondary structure compared to wild-type
G18A/G108A
the mutant shows reduced activity compared to the wild type enzyme
K318E
complete loss of activity
S188A
activity similar to wild-type
S273A
about 30% residual activity, no change in secondary structure compared to wild-type
V186Q
about 55% residual activity
D185G
-
complete loss of activity
-
D341G
-
about 10% residual activity, no change in secondary structure compared to wild-type
-
K318E
-
complete loss of activity
-
S188A
-
activity similar to wild-type
-
S273A
-
about 30% residual activity, no change in secondary structure compared to wild-type
-
D198N
-
9% activity of the wild type enzyme