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3.1.26.5: ribonuclease P

This is an abbreviated version!
For detailed information about ribonuclease P, go to the full flat file.

Word Map on EC 3.1.26.5

Reaction

endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor =

Synonyms

Aq_880, aRpp29, aRpp29 protein, AtPop1p, AtPRORP1, AtPRORP2, AtPRORP3, C5 protein, CrPRORP, hPOP1, hPOP4, hPOP7, M1 RNA, M1GS, M1GS RNA, mitochondrial RNase P protein 1, MRPP1, MRPP2, MRPP3, nuclear ribonclease P ribonucleoprotein, nuclease, ribo-, P, Pfu Pop5, PhoPRNA, POP1, Pop1p, Pop5, Pop6, Pop7, PRORP, PRORP1, PRORP2, PRORP3, Protein C5, protein-only ribonuclease P, protein-only RNase P, protein-only RNase P enzyme, proteinaceous RNase P, ribonuclease MRP, ribonuclease P, ribonuclease P ribozyme, ribosomal RNA processing ribonucleoprotein, Ribunuclease P, RNA processing protein POP1, RNA processing protein POP5, RNA processing protein POP6, RNA processing protein POP7, RNA processing protein POP8, RNase MRP, RNase P, RNase P holoenzyme, RNase P protein, RNase P ribozyme, RNase P RNA, RNase P/MRP, RNase P/MRP protein, RNaseP protein, RNaseP protein p20, RNaseP protein p30, RNaseP protein p38, RNaseP protein p40, RNases P, RNP, Rpm2p, RPP, RPP14, Rpp20, Rpp21, Rpp25, Rpp29, Rpp30, Rpp38, RPP40, RPR, RPR1, transfer RNA 5' maturation enzyme, transfer RNA processing enzyme, tRNA processing enzyme, tRNA-processing endonuclease, tRNA-processing enzyme

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.5 ribonuclease P

Cloned

Cloned on EC 3.1.26.5 - ribonuclease P

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtPRORP3 confers viability to Escherichia coli cells with a lethal knockdown of its endogenous RNA-based RNase P
cDNAs of full-length Rpp20 and Rpp25 cloned into pCR4-TOPO. Full-length Rpp25 (encompassing residues 1-199) subcloned with an N-terminal hexahistidine tag into a pPROEX-HTb expression vector using Nco1/Not1 restriction sites. Full-length Rpp20 (residues 1-140) subcloned into a pET-30 expression vector. The pET-30 vector modified to bear a TEV cleavage site to remove the N-terminal hexahistidine tag. Mutant Rpp20(35-140) amplified from pET-30 Rpp20 to introduce 5' Asc1 and 3' Not1 sites and then ligated into a modified version of pET-15b vector with an Asc1 site inserted directly after the his tag. Fragments Rpp20, mutant Rpp20(16-140), mutant Rpp25(25-170) and co-expressed Rpp20/Rpp25 (in which Rpp20 bears the his tag) subcloned in pETDuet-1 vector with an N-terminal hexahistidine tag. Mutant Rpp25(25-170) also subcloned in a pCDFDuet-1 vector also with an N-terminal hexahistidine tag. Rpp25, Rpp20 (from pET-30 vector) and mutant Rpp20(35-140) expressed in Escherichia coli strain BL21(DE3)pLysS. All the other proteins subcloned into pETDuet-1 or pCDFDuet-1 vectors expressed in Escherichia coli KRX strain
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chromosomal localization of genes encoding the RNase P subunits
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cloning of mutant enzyme in amphotropic packaging PA317 cells, heterologous expression of wild-type and mutant enzymes in human H9 cells
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construction of a Bacillus subtilis mutant strain that conditionally expresses the RNase P protein under control of the xylose promoter (Pxyl)
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DNA and amino acid sequence determination and analysis of two cDNAs encoding two putative protein subunits of potato ribonuclease P, StPop5 and StRpp25, subcloning in Escherichia coli strain M15, recombinant overexpression as His6-tagged proteins in Escherichia coli strain BL21(DE3)
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DNA and amino acid sequence determination and analysis, in vitro transcription, and overexpression in Escherichia coli strain BL21(DE3)
DNA sequence coding for mutants M1-C1 and M1-C2 cloned into vector pU6, which contains a GFP expression cassette, and placed under the control of the small nuclear U6 RNA promoter. DNA sequence for ribozyme M1-thymidine kinase, which is derived from V57 and targets the herpes simplex virus 1 thymidine kinase mRNA, also cloned into vector pU6. Transformed into Salmonella typhimurium aroA strain SL7207
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Escherichia coli transformed with a plasmid expressing RNase P RNA
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expressed in Escherichia coli Rosetta BL21(DE3) cells
expressed in Escherichia coli strain JM109
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expressed in Escherichia coli, TOPO-TA cloning vector, in vitro transcription
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expression in Escherichia coli BL21
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expression in Escherichia coli BL21(DE3)
expression in Escherichia coli. The enzyme rescues the growth of Escherichia coli and Saccharomyces cerevisiae strains with inactivations of their more complex and larger endogenous ribonucleoprotein RNase P
expression in Escherichia coli. the protein is homologous to the human RNase P protein Rpp29, yeast RNase P protein Pop4, and an archaeal RNase P protein from Methanobacter thermoautotrophicus
expression of His-tagged enzyme, co-expression of C-terminally TAP-tagged RNaseP with C-terminally TAP-tagged yeast RNase MRP
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expression of His-tagged protein subunits Rpp29, Rpp21, and RNA subunit H1 in Escherichia coli BL21(DE3)
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expression of His-tagged subunits Rpp14, Rpp20, Rpp21, Rpp25, Rpp29, Rpp30, Rpp38, Rpp40, and hPop5 in Escherichia coli
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expression of the protein and RNA components of mycobacterial RNase P in Escherichia coli
expression of the protein subunit in Escherichia coli. The cloned protein is reconstituted with the RNA subunit transcribed in vitro
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expression of wild-type and mutant enzymes in Escherichia coli
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expression of wild-type and mutant external guide sequences in brief, amphotropic PA317 cells
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expression of wild-type enzyme and protein subunit Rpp25 in Escherichia coli
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expression of wild-type enzyme by in vitro transcription, expression of His-tagged enzyme
full-length Pop6 and Pop7 coexpressed in Escherichia coli. Selenomethionine derivative of the Pop6-Pop7 heterodimer obtained using Escherichia coli strain BL21
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gene At2g47290, DNA and amino acid sequence determination and analysis, AtPop1 mRNA is present in multiple splicing isoforms, possibly encoding several distinct proteins, splicing patterns of AtPop1 mRNAs, overview. Recombinant expression in Escherichia coli strain Rosetta (DE3)pLysS
L0N807
gene for the protein moiety
gene for the RNA component
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gene for the RNA subunit
genes PRORP1 and PRORP2, recombinant expression of C-terminally His6-tagged PRORP1 and of C-terminally His6-tagged and N-terminally GST-tagged PRORP2 in Escherichia coli
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genes rnpA and rnpB, recombinant expression of GST-tagged wild-type and mutant proteins and RNA in Escherichia coli strain BL21(DE3)pLysS
N-terminal His-tagged RNase P protein overexpressed
organellar (AtPRORP1) and the two nuclear (AtPRORP2,3) single-polypeptide RNase P isoenzymes from Arabidopsis thaliana confer viability to Escherichia coli cells with a lethal knockdown of its endogenous RNA-based RNase P
overexpression in Escherichia coli
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overexpression in Escherichia coli BL21(DE3) Rosetta cells
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overexpression of native and selenomethionine-containing wild-type protein subunit Ph1877p, and mutant protein subunits Ph1788p in Escherichia coli
overexpression of the protein subunit in Escherichia coli
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overproduced in Escherichia coli
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pET-15b-derived plasmid endcoding the C5 protein expressed in Escherichia coli BL21(DE3). Into pUC19 vector and transformed into Escherichia coli strain JM109
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plasmids carrying the M1GS constructs delivered to murine cytomegalovirus-infected mice by a hydrodynamic transfection procedure
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PRORP1-PRORP3, phylogenetic analysis, overview
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protein overexpressed in Escherichia coli with an N-terminal hexahistidyl tag, RNA subunit produced by run-off in vitro T7 transcription from plasmid pDW98 linearized with BsaAI
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ribonuclease P protein components 1, 2, 3 and 4 are expressed in Escherichia coli cells, and the resulting proteins Ph1481p, Ph1601p, Ph1771p, and Ph1877p are purified to apparent homogeneity in a set of column chromatographies. RNase P reconstituted of four proteins with the in vitro transcripted Pyrococcus horikoshii RNase P RNA exhibits enzymatic properties like those of native enzyme
RPP21 and RPP29. RPP29/pET-33b plasmid transformed into Escherichia coli BL21(DE3) Rosetta cells
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subunits DRpp29 and RNase P
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tagged overexpression of MRPP1, MRPP2 and MRPP3 in human cells
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the enzyme confers viability to Escherichia coli cells with a lethal knockdown of its endogenous RNA-based RNase P
the RNase P ribozyme subunit is expressed in Escherichia coli BL21(DE3) cells
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