3.1.25.1: deoxyribonuclease (pyrimidine dimer)
This is an abbreviated version!
For detailed information about deoxyribonuclease (pyrimidine dimer), go to the full flat file.
Word Map on EC 3.1.25.1
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3.1.25.1
-
glycosylase
-
strand
-
comet
-
thymine
-
genotoxic
-
formamidopyrimidine
-
cyclobutane
-
formamidopyrimidine-dna
-
incision
-
abasic
-
uv-induced
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endonucleases
-
uv-irradiated
-
photoproducts
-
duplex
-
xeroderma
-
pigmentosum
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dimer-specific
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endoiii
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8-oxoguanine
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beta-elimination
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8-oxog
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repair-deficient
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alkali-labile
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apyrimidinic
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ultraviolet-irradiated
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photolyase
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lesion-specific
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apurinic
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uv-damaged
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helix-hairpin-helix
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iii-sensitive
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dna-glycosylase
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7,8-dihydro-8-oxoguanine
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fpg-sensitive
-
8-hydroxyguanine
-
3-methyladenine-dna
-
uracil-dna
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5-hydroxycytosine
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incises
-
cis-syn
-
dipyrimidine
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medicine
-
molecular biology
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repair-proficient
-
dimer-containing
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enzyme-dna
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8-oxo-7,8-dihydroguanine
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analysis
-
mitogenome
-
excision-repair
-
photoreactivation
-
5-hydroxyuracil
- 3.1.25.1
- glycosylase
- strand
- comet
- thymine
-
genotoxic
- formamidopyrimidine
-
cyclobutane
-
formamidopyrimidine-dna
-
incision
-
abasic
-
uv-induced
- endonucleases
-
uv-irradiated
- photoproducts
- duplex
- xeroderma
- pigmentosum
-
dimer-specific
-
endoiii
- 8-oxoguanine
-
beta-elimination
-
8-oxog
-
repair-deficient
-
alkali-labile
-
apyrimidinic
-
ultraviolet-irradiated
-
photolyase
-
lesion-specific
-
apurinic
-
uv-damaged
-
helix-hairpin-helix
-
iii-sensitive
-
dna-glycosylase
- 7,8-dihydro-8-oxoguanine
-
fpg-sensitive
- 8-hydroxyguanine
-
3-methyladenine-dna
-
uracil-dna
- 5-hydroxycytosine
-
incises
-
cis-syn
-
dipyrimidine
- medicine
- molecular biology
-
repair-proficient
-
dimer-containing
-
enzyme-dna
- 8-oxo-7,8-dihydroguanine
- analysis
-
mitogenome
-
excision-repair
-
photoreactivation
- 5-hydroxyuracil
Reaction
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate =
Synonyms
ApeI, bacteriophage T4 endodeoxyribonuclease V, bacteriophage T4 endonuclease V, correndonuclease I, correndonuclease II, endodeoxyribonuclease (pyrimidine dimer), endonuclease II, endonuclease III, endonuclease V, Escherichia coli UV endonuclease, excision endonuclease UvrABC, excision nuclease UvrABC, More, nuclease V, bacteriophage T4 endodeoxyribonuclease V, nuclease, Escherichia coli UV-endodeoxyribonuclease, Py-Py-correndonuclease, ribosomal protein S3, rpS3, T4 Endo V, T4 endonuclease V, T4N5, type I AP endonuclease, UvrA, UvrABC nuclease, UvrB, UvrC
ECTree
3.1.25.1 deoxyribonuclease (pyrimidine dimer)Advanced search results
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results (Engineering
Engineering on EC 3.1.25.1 - deoxyribonuclease (pyrimidine dimer)
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D35A
A3K
Tequatrovirus T4
-
substantial level of AP- and dimer specific nicking, not processive
A3L
Tequatrovirus T4
-
substantial level of AP- and dimer specific nicking, not processive
A3Q
Tequatrovirus T4
-
substantial level of AP- and dimer specific nicking, not processive
E23D
E23Q
K130G
K130H
R3Q
Tequatrovirus T4
-
active site mutant, retains full substrate binding ability but shows complex structure differences, crystal structure determination with a bound DNA duplex in comparison to the wild-type enzyme
T127M
Tequatrovirus T4
-
almost same activity as wild-type, Km value in the same order as that of wild-type
T2V
Tequatrovirus T4
-
intermediate activity level of dimer-specific glycosylase and nicking activity
W128S
Y129N
Y131N
additional information
D35A
-
site-directed mutagenesis, the mutant shows no deoxyinosine cleavage activity
D35A
-
site-directed mutagenesis, the mutant shows no deoxyinosine cleavage activity
-
E23D
Tequatrovirus T4
-
no detectable N-glycosylase activity on pyrimidine dimers, partial retention of AP-lyase activity
E23D
Tequatrovirus T4
-
active site mutant, retains full substrate binding ability but shows complex structure differences, crystal structure determination with a bound DNA duplex in comparison to the wild-type enzyme
E23Q
Tequatrovirus T4
-
no detectable N-glycosylase activity on pyrimidine dimers, loss of AP-lyase activity
E23Q
Tequatrovirus T4
-
active site mutant, retains full substrate binding ability but shows complex structure differences, crystal structure determination with a bound DNA duplex in comparison to the wild-type enzyme
K130G
Tequatrovirus T4
-
diminished capacity to make complete incisions, significantly decreased intracellular accumulation
K130G
Tequatrovirus T4
-
low dimer-specific binding activity, AP-endonuclease function several times as efficient as that of the wild-type
K130H
Tequatrovirus T4
-
diminished capacity to make complete incisions, slight decreased intracellular accumulation
K130H
Tequatrovirus T4
-
low dimer-specific binding activity, wild-type lyase activity, reduced glycosylase activity
Y129N
Tequatrovirus T4
-
dimer-specific binding and nicking abolished, apurinic-specific nicking abolished
Y131N
Tequatrovirus T4
-
in combination with Y129N, dimer-specific binding and nicking abolished, ability for apurinic-specific nicking retained
Y131N
Tequatrovirus T4
-
in combination with Y129N, wild-type lyase activity retained, diminished dimer-specific binding and N-glycosylase activity
additional information
Tequatrovirus T4
-
deletion of Arg 3, only marginal level of AP specificity, no other detectable activity
additional information
Tequatrovirus T4
-
insertion of Gly between the first 2 amino acids, lack of dimer-specific glycosylase and nicking activity
