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3.1.21.6: CC-preferring endodeoxyribonuclease

This is an abbreviated version!
For detailed information about CC-preferring endodeoxyribonuclease, go to the full flat file.

Word Map on EC 3.1.21.6

Reaction

Endonucleolytic cleavage to give 5'-phosphooligonucleotide end-products, with a preference for cleavage within the sequence CC =

Synonyms

Deoxyriboendonuclease, DNA-adenine-transferase, EC 3.1.4.30, endodeoxyribonuclease, endonuclease II, endonuclease NX, endonuclease T, Escherichia coli endonuclease II, Nuclease, endodeoxyribo-, R1Bm EN, R1Bm endonuclease, Redoxyendonuclease, Streptomyces glaucescens exocytoplasmic dodeoxyribonuclease, VirD1/D2 endonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.6 CC-preferring endodeoxyribonuclease

Engineering

Engineering on EC 3.1.21.6 - CC-preferring endodeoxyribonuclease

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D19A
-
relative activity of bottom-strand cleavage is less than half that of top-strand cleavage, whereas activity of top-strand cleavage is more than 80% that of the wild-type
E147A
-
does not exhibit a significant reduction in nicking activity, compared with that of the wild-type
E18A
-
relative activity of bottom-strand cleavage is less than half that of top-strand cleavage, whereas activity of top-strand cleavage is more than 80% that of the wild-type
H130A
-
moderately decreased nicking activity
H140A
-
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
H209A
-
exhibits no detectable cleavage activity on the top or bottom strand of the oligonucleotide substrate
H54A
-
relative activity of bottom-strand cleavage is less than half that of top-strand cleavage, whereas activity of top-strand cleavage is more than 80% that of the wild-type
H78A
-
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
K56A
-
moderately decreased nicking activity
N180A
-
altered cleavage patterns, shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
Q97A
-
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
R139A
-
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
R144A
-
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
S206A
-
relative activity of bottom-strand cleavage is less than half that of top-strand cleavage, whereas activity of top-strand cleavage is more than 80% that of the wild-type
S43A
-
does not exhibit a significant reduction in nicking activity, compared with that of the wild-type
S99A
-
does not exhibit a significant reduction in nicking activity, compared with that of the wild-type
T178A
-
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
Y42A
-
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates
Y98A
-
altered cleavage patterns, shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates