3.1.2.4: 3-hydroxyisobutyryl-CoA hydrolase
This is an abbreviated version!
For detailed information about 3-hydroxyisobutyryl-CoA hydrolase, go to the full flat file.
Word Map on EC 3.1.2.4
Reaction
Synonyms
3-hydroxy-isobutyryl CoA hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, BCH, beta-hydroxyisobutyryl-CoA hydrolase, beta-hydroxyisobutyryl-coenzyme A hydrolase, CHY1, HIB CoA hydrolase, HIB-CoA hydrolase, HIBCH, HIBYL-CoA hydrolase, HICH, hydrolase, 3-hydroxyisobutyryl coenzyme A, More, NGR_b20860, Smb20752, VutE
ECTree
3.1.2.4 3-hydroxyisobutyryl-CoA hydrolaseAdvanced search results
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results (Cloned
Cloned on EC 3.1.2.4 - 3-hydroxyisobutyryl-CoA hydrolase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
CHY1 cDNA cloned behind the 35S promoter in a binary vector, introduced into homozygous chy1-10 mutant plants by Agrobacterium tumefaciens strain GV3101 transformation
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme
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open-reading frame of the Chy1 gene amplified, cDNA ligated into the vector pEX5-CT/TOPO TA to create a fusion of the open-reading frame with a His tag-coding extension at the C-terminus. Plasmid transferred into Escherichia coli BL21 (DE3) cells for expression
subcloning in Escherichia coli strain XL1-Blue, and expression in Escherichia coli strain BL21 (DE3) under control of the T7 promoter, co-expression with the beta-ketothiolase gene from Ralstonia eutropha strain H16 and the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from Ralstonia eutropha strain H16, or Clostridium acetobutylicum strain ATCC824 leads to biosynthesis of enantiomerically pure (S)-3-hydroxybutyric acid in the engineered Escherichia coli cells, extracellular product, method optimization, overview
