3.1.11.5: exodeoxyribonuclease V
This is an abbreviated version!
For detailed information about exodeoxyribonuclease V, go to the full flat file.
Word Map on EC 3.1.11.5
Reaction
Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides
=
Synonyms
AddAB enzyme, E. coli ATP-dependent DNase, E. coli exonuclease V, Escherichia coli exonuclease V, Escherichia coli RecBCD, Exodeoxyribonuclease V 125 kDa polypeptide, Exodeoxyribonuclease V 135 KDA polypeptide, Exodeoxyribonuclease V 67 kDa polypeptide, exonuclease V, ExoV, gene recBC DNase, gene RecBC endoenzyme, nuclease, exodeoxyribo V, PAB2263, RecB, RecB helicase-nuclease, REcB30 protein, recBC deoxyribonuclease, recBC DNase, recBC nuclease, RecBCD, RecBCD DNase, recBCD enzyme, RecBCD exonuclease, RecBCD-type helicase-nuclease, RecD, UPF0286 protein PYRAB01260
ECTree
Metals Ions
Metals Ions on EC 3.1.11.5 - exodeoxyribonuclease V
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Cd2+
-
inhibitory in the presence of Mg2+
Cu2+
-
slight activation of ssDNA nuclease activity of RecB30
Ni2+
-
slight activation of ssDNA nuclease activity of RecB30
Zn2+
-
slight activation of ssDNA nuclease activity of RecB30
Ca2+
-
inhibitory in the presence of Mg2+
Ca2+
-
inhibitory on helicase activity in the presence of Mg2+
Ca2+
-
DNA-dependent ATPase unaffected
Ca2+
-
double-stranded DNA exonuclease and single-stranded DNA exonuclease and endonuclease activities completely inhibited
Ca2+
-
slight activation of ssDNA nuclease activity of RecB30
Co2+
-
stimulates at 1-2 mM
Co2+
-
activation of ssDNA nuclease activity of RecB30
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
produces intermediates
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
the nuclease activities of wild-type enzyme is sensitive to the concentration of free magnesium ions in solution
Mg2+
-
activation of ssDNA nuclease activity of RecB30
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mg2+
-
required, optimal concentration around 3-12 mM
Mn2+
-
less effective than Mg2+
Mn2+
-
more effective as Mg2+
Mn2+
-
less effective than Mg2+
Mn2+
-
less effective than Mg2+
Mn2+
-
less effective than Mg2+
Mn2+
-
less effective than Mg2+
Mn2+
-
inhibitory in the presence of Mg2+
Mn2+
-
activation of ssDNA nuclease activity of RecB30
Mn2+
-
less effective than Mg2+
Mn2+
-
less effective than Mg2+
Mn2+
-
less effective than Mg2+
Mn2+
-
less effective than Mg2+
Mn2+
-
less effective than Mg2+