3.1.1.74: cutinase
This is an abbreviated version!
For detailed information about cutinase, go to the full flat file.
Word Map on EC 3.1.1.74
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3.1.1.74
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fusarium
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solani
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pi
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lipase
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terephthalate
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p-nitrophenyl
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lipolytic
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thermobifida
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esterases
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insolens
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fusca
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humicola
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ideonella
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polybutylene
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sakaiensis
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industry
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polycaprolactone
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tributyrin
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degradation
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sulfosuccinate
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haematococca
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monilinia
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terephthalic
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petase
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synthesis
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nectria
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hydrophobins
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saccharomonospora
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biotechnology
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environmental protection
- 3.1.1.74
- fusarium
- solani
- pi
- lipase
- terephthalate
- p-nitrophenyl
-
lipolytic
- thermobifida
- esterases
- insolens
- fusca
-
humicola
-
ideonella
-
polybutylene
- sakaiensis
- industry
-
polycaprolactone
- tributyrin
- degradation
- sulfosuccinate
- haematococca
-
monilinia
-
terephthalic
- petase
- synthesis
- nectria
-
hydrophobins
-
saccharomonospora
- biotechnology
- environmental protection
Reaction
Synonyms
acidic cutinase, CcCUT1, CDEF1, CLE, Cut 5a, cut-2.KW3, Cut1, Cut11, Cut190, Cut2, Cut5a, CUTAB1, CutB, cuticle destructing factor 1, cutin esterase, cutin hydrolase, cutinase, cutinase 1, cutinase 2, cutinase-1, cutinase-like enzyme, cutinolytic polyesterase, CutL, CutL1, FspC, fungal cutinase, HIc, LC-cutinase, More, MYCTH_2110987, PET hydrolase, Tfu_0883, Thcut1, THCUT1 protein, Thc_Cut1, Thc_Cut2, TRIREDRAFT_60489
ECTree
3.1.1.74 cutinaseAdvanced search results
results (
results (KCat KM Value
KCat KM Value on EC 3.1.1.74 - cutinase
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
7.7
4-nitrophenyl caprylate
-
pH 8.0, 25°C, recombinant enzyme
6.4
4-nitrophenyl myristate
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pH 8.0, 25°C, recombinant enzyme
0.00000167
4-nitrophenyl acetate
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in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
0.00004217
4-nitrophenyl acetate
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in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
5
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant Q65E
9
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant wild-type isozyme
13
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant L183A
30
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant R19S
42
4-nitrophenyl acetate
fusion protein Cut1-HFB4, pH 7.0, 25°C
42.4
4-nitrophenyl acetate
pH and temperature not specified in the publication
44
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant R187K
55
4-nitrophenyl acetate
fusion protein Cut1-HFB7, pH 7.0, 25°C
65
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant R19S/R29N/A30V
71
4-nitrophenyl acetate
fusion protein Cut1-HFB9b, pH 7.0, 25°C
76
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant R29N/A30V
153
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant A30V
159
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant R29N
291
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant isozyme
62
4-nitrophenyl butanoate
fusion protein Cut1-HFB9b, pH 7.0, 25°C
68
4-nitrophenyl butanoate
fusion protein Cut1-HFB4, pH 7.0, 25°C
130
4-nitrophenyl butanoate
fusion protein Cut1-HFB7, pH 7.0, 25°C
0.00000043
4-nitrophenyl butyrate
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in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
0.0000582
4-nitrophenyl butyrate
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in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
5
4-nitrophenyl butyrate
pH 7.0, 25°C, recombinant wild-type isozyme
258
4-nitrophenyl butyrate
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pH 8.0, 70°C, recombinant mutant C275A/C292A, absence of 1% PEG
425
4-nitrophenyl butyrate
pH 7.0, 25°C, recombinant isozyme
888
4-nitrophenyl butyrate
-
pH 8.0, 70°C, recombinant wild-type enzyme, absence of 1% PEG
1046
4-nitrophenyl butyrate
-
pH 8.0, 30°C, recombinant wild-type enzyme, presence of 1% PEG
1050
4-nitrophenyl butyrate
-
pH 8.0, 30°C, recombinant wild-type enzyme, absence of 1% PEG
1090
4-nitrophenyl butyrate
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pH 8.0, 50°C, recombinant mutant C275A/C292A, absence of 1% PEG
1295
4-nitrophenyl butyrate
-
pH 8.0, 70°C, recombinant wild-type enzyme, absence of 1% PEG
1311
4-nitrophenyl butyrate
-
pH 8.0, 50°C, recombinant wild-type enzyme, presence of 1% PEG
1370
4-nitrophenyl butyrate
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pH 8.0, 30°C, recombinant mutant C275A/C292A, absence of 1% PEG
1630
4-nitrophenyl butyrate
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pH 8.0, 50°C, recombinant wild-type enzyme, absence of 1% PEG
0.0000023
4-nitrophenyl hexanoate
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in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
0.000023
4-nitrophenyl hexanoate
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in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
0.00001017
4-nitrophenyl valerate
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in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
0.000053
4-nitrophenyl valerate
-
in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol
