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3.1.1.74: cutinase

This is an abbreviated version!
For detailed information about cutinase, go to the full flat file.

Word Map on EC 3.1.1.74

Reaction

cutin
+
H2O
= 2 cutin monomers

Synonyms

acidic cutinase, CcCUT1, CDEF1, CLE, Cut 5a, cut-2.KW3, Cut1, Cut11, Cut190, Cut2, Cut5a, CUTAB1, CutB, cuticle destructing factor 1, cutin esterase, cutin hydrolase, cutinase, cutinase 1, cutinase 2, cutinase-1, cutinase-like enzyme, cutinolytic polyesterase, CutL, CutL1, FspC, fungal cutinase, HIc, LC-cutinase, More, MYCTH_2110987, PET hydrolase, Tfu_0883, Thcut1, THCUT1 protein, Thc_Cut1, Thc_Cut2, TRIREDRAFT_60489

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.74 cutinase

Inhibitors

Inhibitors on EC 3.1.1.74 - cutinase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(O,O)-diethyl-(3,5,6-trichloro)-2-pyridylphosphorothioate
-
1 mM, 90% inhibition
1-Heptanol
27% inhibition at 40% v/v; 28% inhibition at 40% v/v
1-Hexanol
68% inhibition at 40% v/v; 86% inhibition at 40% v/v
2,3,5-trichloropyridine-6-(O-methyl-O-n-butyl)-phosphate ester
-
i.e. MAT 9564
2-mercaptoethanol
-
slight inhibition at 10 mM
2-[(ethylsulfanyl)methyl]phenyl hydrogen methylcarbonimidate
-
-
3-(4-mercaptobutylthio)-1,1,1-trifluoro-2-propanone
-
-
3-n-octylthio-1,1,1-trifluoro-2-propanone
-
-
3-phenethylthio-1,1,1-trifluoropropan-2-one
3-phenylthio-1,1,1-trifluoropropan-2-one
-
4-nitrophenyl P-methyl-N-octylphosphonamidoate
-
-
6-mercaptohexyl acrylic ester
-
product inhibition
acetone
-
92% inhibition at 75% v/v
AgNO3
90% inhibition at 1 mM; 93% inhibition at 1 mM
alkylboronic acids
-
-
ANS
binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm by up to 7°C
Ba2+
-
inhibits isozyme Tfu 0882 7%
BaCl2
14% inhibition at 1 mM; 8% inhibition at 1 mM
Benzene
77% inhibition at 40% v/v; 79% inhibition at 40% v/v
butanol
10% inhibition at 40% v/v; 17% inhibition at 40% v/v
butyl 4-nitrophenyl undecylphosphonate
in the absence of surfactant, the rate of cutinase inhibition is very low. The addition of beta-octylglucoside is required to trigger the inhibition of cutinase, which is completely inactivated after 60 min
Carbaryl
-
-
carbofuran
-
-
chlorpyrifos-methyl
-
upon chloroperoxidase oxidation, chlorpyrifos-methyl shows a very strong cutinase inhibition as compared to the corresponding oxon standard
chlorpyrifos-methyl oxon
-
-
CrCl2
99% inhibition at 1 mM; 99% inhibition at 1 mM
CuSO4
26% inhibition at 1 mM; 32% inhibition at 1 mM
D-glucose
Thcut1 mRNA is repressed by glucose
deoxycholate
24% inhibition at 10 mM; 25% inhibition at 10 mM
Dichloromethane
Diethyl p-nitrophenyl phosphate
diethyl-p-nitrophenyl phosphate
-
diethyldicarbonate
54% inhibition at 1 mM; 56% inhibition at 1 mM
diisopropyl fluorophosphate
dioxan
-
23% inhibition at 75% v/v
DMSO
-
49% inhibition at 75% v/v
E600
in the absence of surfactant, no inhibition is observed with E600. The addition of beta-octylglucoside is required to trigger the inhibition of cutinase, which is completely inactivated after 12 min
EDTA
10 mM, 42% residual acivity
ethanol
-
97% inhibition at 75% v/v
ethylene glycol
cleavage product accumulation decreases the activity of cutinase during PET hydrolysis
Fe3+
10 mM, 43% residual acivity
FeSO4
55% inhibition at 1 mM; 55% inhibition at 1 mM
glycerol
guanidine hydrochloride
hexyl acetate
63% inhibition at 40% v/v; 83% inhibition at 40% v/v
HgCl2
99% inhibition at 1 mM; 99% inhibition at 1 mM
Isopropanol
-
complete inhibition at 75% v/v
K+
1 mM, 42% residual acivity
methanol
methomyl
-
-
MgCl2
15% inhibition at 1 mM; 8% inhibition at 1 mM
Mn2+
-
slight inhibition at 1 mM
n-hexane
Na+
1 mM, 39% residual acivity
O-(4-nitrophenyl) S-octyl methylphosphonothioate
-
-
O-methyl-O-(p-nitrophenyl)octylphosphonate
-
-
O-octyl-O-(p-nitrophenyl)ethylphosphonate
-
-
O-octyl-O-(p-nitrophenyl)methylphosphonate
-
-
oxidized malathion
-
oxidized malathion, contrarily to malaoxon, reveals cutinase inhibition
paraoxon
paraoxon-methyl
-
-
PbCl2
53% inhibition at 1 mM; 54% inhibition at 1 mM
phenylboronic acid
-
5 mM, 63% inhibition, competitive
primicarb
-
-
propoxur
-
-
RbCl
93% inhibition at 1 mM; 95% inhibition at 1 mM
sodium bis(2-ethylhexyl)ester sulfosuccinic acid
-
pseudo-competitive inhibitor
sodium deoxycholate
-
-
sodium dioctyl sulfosuccinate
-
-
Sodium dodecyl sulfate
-
competitive, detailed study of interaction with enzyme. At molar ratio of SDS:enzyme of about 10, formation of aggregates which include more than one protein molecule. At higher concentration of SDS, denaturation of protein, denatured state of enzyme is unusually compact
triethylamine
24% inhibition at 40% v/v
Triton X-100
35% inhibition at 1 mM; 55% inhibition at 1 mM
Tween 80
10 mM, 48% residual acivity
Tween-20
28% inhibition at 1 mM; 35% inhibition at 1 mM
Tween-80
24% inhibition at 1 mM; 60% inhibition at 1 mM
ZnSO4
59% inhibition at 1 mM; 65% inhibition at 1 mM
additional information
-