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3.1.1.74: cutinase

This is an abbreviated version!
For detailed information about cutinase, go to the full flat file.

Word Map on EC 3.1.1.74

Reaction

cutin
+
H2O
= 2 cutin monomers

Synonyms

acidic cutinase, CcCUT1, CDEF1, CLE, Cut 5a, cut-2.KW3, Cut1, Cut11, Cut190, Cut2, Cut5a, CUTAB1, CutB, cuticle destructing factor 1, cutin esterase, cutin hydrolase, cutinase, cutinase 1, cutinase 2, cutinase-1, cutinase-like enzyme, cutinolytic polyesterase, CutL, CutL1, FspC, fungal cutinase, HIc, LC-cutinase, More, MYCTH_2110987, PET hydrolase, Tfu_0883, Thcut1, THCUT1 protein, Thc_Cut1, Thc_Cut2, TRIREDRAFT_60489

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.74 cutinase

Expression

Expression on EC 3.1.1.74 - cutinase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CDEF1 gene is expressed in leaves of transgenic 35S::CDEF1 plants. CDEF1 gene is highly expressed in mature pollen
CDEF1 gene is not expressed in leaves of wild-type plants. Is expressed at low levels in roots
cutinase genes show four differential expression patterns, indicating regulatory sub- and neo-functionalization
-
growth on agro industrial wastes can be used as inducer of cutinase production, e.g. peels of multi green colored watermelon
increased enzyme production in strain WSH03-11 by induction with cutin, cutin hydrolysis monomers, and some lipids, e.g. tomato peel
increased enzyme production in wild-type strain by induction induction with cutin, cutin hydrolysis monomers, and some lipids, e.g. olive oil
increased enzyme production in wild-type strain by induction with cutin, cutin hydrolysis monomers, and some lipids, e.g. apple cutin
increased enzyme production in wild-type strain by induction with cutin, cutin hydrolysis monomers, and some lipids, e.g. tomato peel
-
mutants carrying a DELTActf1 loss-of-function allele grown on inducing substrates fail to activate extracellular cutinolytic activity and expression of the cut1 gene, encoding a putative cutinase
the recombinant enzyme is induced with 0.5% v/v methanol in basal salt medium at pH 5.0 and 28°C
-
the zinc finger transcription factor Ctf1 from Fusarium oxysporum mediates efficient transcriptional activation of cut1 and lip1 on fatty acid substrates, but not on glucose. The Ctf1 transcription factor, a functional orthologue of CTF1alpha, controls expression of cutinase genes and virulence. Strains harbouring a ctf1C allele in which the ctf1 coding region is fused to the strong constitutive Aspergillus nidulans gpdA promoter show increased induction of cutinase activity and gene expression. Higher levels of cut1 transcript in mycelia of the wild-type grown on apple cutin than in those grown on glucose. Activation of cut1 by apple cutin is even more pronounced in the ctf1C strain than in the wild-type. Low expression of cut1 in roots 10 days after inoculation of tomato plants