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3.1.1.74: cutinase

This is an abbreviated version!
For detailed information about cutinase, go to the full flat file.

Word Map on EC 3.1.1.74

Reaction

cutin
+
H2O
= 2 cutin monomers

Synonyms

acidic cutinase, CcCUT1, CDEF1, CLE, Cut 5a, cut-2.KW3, Cut1, Cut11, Cut190, Cut2, Cut5a, CUTAB1, CutB, cuticle destructing factor 1, cutin esterase, cutin hydrolase, cutinase, cutinase 1, cutinase 2, cutinase-1, cutinase-like enzyme, cutinolytic polyesterase, CutL, CutL1, FspC, fungal cutinase, HIc, LC-cutinase, More, MYCTH_2110987, PET hydrolase, Tfu_0883, Thcut1, THCUT1 protein, Thc_Cut1, Thc_Cut2, TRIREDRAFT_60489

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.74 cutinase

Cloned

Cloned on EC 3.1.1.74 - cutinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplified DNA fragments cloned into the pGEM-T vector, cut1 isolated from a lambdaEMBL3 genomic library
cloned and expressed in Trichoderma reesei
cloned and overexpressed in Escherichia coli
cloned in pMa5-L and overexpressed in Escherichia coli
-
codon optimized cutinase gene anig5, subcloning of C-terminally His-tagged enzyme in Escherichia coli strain XL1-Blue and recombinant expression in Pichia pastoris after selection from 38 strains for production, screening and pH-Profiling of the cutinase activities of the culture supernatants, overview
-
cutinase S120A is expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli Origami B(DE3)
expressed in Escherichia coli strain Origami B(DE3)
expressed in Pichia pastoris
expressed in Pichia pastoris. No significant differences between the expression of native Thc_Cut1 and of two glycosylation site knock out mutants (Thc_Cut1_koAsn and Thc_Cut1_koST) concerning the total extracellular protein concentration and volumetric activity are observed
expression in Escherichia coli
expression in Escherichia coli WK-6 strain
expression in Komagataella pastoris and Saccharomyces cerevisiae
-
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
Expression of aggregation-prone cutinase protein using RNA polymerase sigma factor (RpoS) or glutathione transferase as fusion partners in Escherichia coli strain BL21 (DE3).
-
expression of cutinase FspC in Bacillus subtilis
-
expression of cutinase isozymes Tfu 0882 and Tfu 0883 in Escherichia coli strain BL21(DE3)
-
gene cut1, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), secretion into periplasm and medium, method development and optimization for process for large-scale production of cutinase in soluble form
gene cut2, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), secretion into periplasm and medium, method development and optimization for process for large-scale production of cutinase in soluble form
gene hic, recombinant expression in Pichia pastoris strain X-33 and secretion to the culture medium
-
gene ScCut1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant of C-terminally His-tagged codon-optimized enzyme expression in Pichia pastoris strain X-33, subcloning in Escherichia coli
gene SsCut, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21, expression in Nicotiana benthamiana leaves, quantitative real-time polymerase chain reaction analysis
heterologous enzyme expression, mostly with secretion to the medium, in different hosts, e.g. Escherichia coli strain W6, Bacillus subtilis, Saccharomyces cerevisiae strain SU50, Pichia pastoris, Aspergillus awamori, and Fusarium venenatum, from different plasmids and using different promoters, and using different signal peptides, e.g. alkaline phosphatase (PhoA) signal peptide, LipA signal peptide, alpha-factor signal peptide, and Fusarium solani cutinase signal peptide, overview. Optimization of method and culture conditions
heterologous expression in Nicotiana tabacum var. Petite Havana using chloroplast transformation vector pLD-cut, modification of chloroplast ultrastructures in cutinase transplastomic lines, phenotype, overview
-
heterologously expressed in Pichia pastoris using the strong methanol-induced AOX I promoter. The protein is expressed with the Saccharomyces cerevisiae alpha factor tag on the N-terminal for extracellular secretion and the six histidine tag on the C-terminus for ease of purification using affinity chromatography
high-level overexpression of Glomerella cingulata cutinase, with a cutinase production of 3800 mg/l and an activity of 434 U/ml, in dense cultures of Pichia pastoris strain X-33 grown under fed-batch conditions, method evaluation, overview
-
into pPICZalphaA with the Saccharomyces cerevisiae alpha-factor signal sequence and methanol-inducible alcohol oxidase promoter, additional sequences of the c-myc epitope and (His)6-tag of the vector fused to the C-terminus of cutinase, while the other expression vector is constructed without any additional sequence. Expressed in Pichia pastoris strain X-33
mutant H204N overexpressed in Escherichia coli Origami B(DE3)
over-expression of recombinant cutinase cloned in pMac5-8 and its variants (S54D, L153Q and T179C) are performed with Escherichia coli WK6 strain
-
overexpression in Escherichia coli strain WK-6
-
overexpression of recombinant cutinase and its variants is performed with strain Escherichia coli WK-6 (delta(lac-pro) galE strA F'[lacIq ZdeltaM15 proAB+])
-
PCR product digested with EcoRI and NotI, and ligated in corresponding sites of the linearized pPICZalphaA vector. Transformation of Escherichia coli DH5alpha cells. Wild-type and mutants heterologously expressed in Pichia pastoris X-33 cells under the control of the methanol-inducible AOX1 promoter
recombinant expression in Escherichia coli strain BL21-Gold(DE3)
recombinant expression of C-terminally His-tagged wild-type and mutant cutinase lacking the signal peptide sequence in Escherichia coli strain BL21(DE3), the enzyme is mostly secreted to the medium. Compared to the cells expressing the inactive cutinase mutant S130A, the cells expressing the truncated cutinase show increased membrane permeability and irregular morphology
-
recombinant expression of cutinase in Saccharomyces cerevisiae strain SU50
-
recombinant expression of extracellular wild-type and mutant enzymes in Escherichia coli
-
recombinant expression via type II secretory system in Escherichia coli strain BL21(DE3), the protein accumulates in the periplasmic space. The alpha-hemolysin secretion system can export target proteins directly from cytoplasm across both cell membrane of Escherichia coli to the culture medium and is therefore used for expression of the extracellular enzyme, method optimization for large-scale industrial production, overview
-
S226P/R228S/S176A with an N-terminal polyhistidine tag is overexpressed in Escherichia coli
sequence comparisons, functional recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Origami B (DE3)
transgenic Arabidopsis plants expressing GFP-CDEF1 fusion protein driven by the 35S promoter. CDEF1 expressed with a 6 x His tag at its C-terminus in suspension cultures of BY-2 tobacco cells
two genes, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), production and assay method optimization, overview
-