3.1.1.74: cutinase

This is an abbreviated version!
For detailed information about cutinase, go to the full flat file.

Reaction

Synonyms

acidic cutinase, CcCUT1, CDEF1, CLE, Cut 5a, cut-2.KW3, Cut1, Cut11, Cut190, Cut2, Cut5a, CUTAB1, CutB, cuticle destructing factor 1, cutin esterase, cutin hydrolase, cutinase, cutinase 1, cutinase 2, cutinase-1, cutinase-like enzyme, cutinolytic polyesterase, CutL, CutL1, FspC, fungal cutinase, HIc, LC-cutinase, More, MYCTH_2110987, PET hydrolase, Tfu_0883, Thcut1, THCUT1 protein, Thc_Cut1, Thc_Cut2, TRIREDRAFT_60489

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.74 cutinase

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Results	in table
22	Activating Compound
7371	AA Sequence
53	Application
1	CAS Registry Number
53	Cloned(Commentary)
23	Crystallization (Commentary)
16	Expression
158	General Information
13	General Stability
141	Inhibitors
63	kcat/KM [mM/s]
4	Ki Value [mM]
142	KM Value [mM]
70	Localization
28	Metals/Ions
52	Molecular Weight [Da]
89	Natural Substrates/ Products (Substrates)
19	Organic Solvent Stability
236	Organism
54	PDB ID
50	pH Optimum
23	pH Range
16	pH Stability
10	pI Value
12	Posttranslational Modification
298	Protein Variants
43	Purification (Commentary)
1	Reaction
4	Reaction Type
215	Reference
2	Renatured (Commentary)
28	Source Tissue
67	Specific Activity [micromol/min/mg]
2	Storage Stability
495	Substrates and Products (Substrate)
48	Subunits
124	Synonyms
1	Systematic Name
46	Temperature Optimum [°C]
13	Temperature Range [°C]
70	Temperature Stability [°C]
89	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.1.1.74 - cutinase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

16-hydroxyhexadecanoic acid

Trichoderma harzianum

A8QPD8

is strongly induced in vitro by cutin monomer 16-hydroxyhexadecanoic acid

691941

DMF

2 entries

DMSO

2 entries

N,N-diethyl-2-phenylacetamide

Thermobifida fusca

-

increases hydrolysis rates of semi-crystalline poly(ethylene terephthalate) films and fabrics for cutinase. The outer layers of the polymer will be better exposed to the enzyme in the presence of N,N-diethyl-2-phenylacetamide. Overall enhancement in colour depth of 300%

709196

olive oil

Trichoderma harzianum

A8QPD8

Thcut1 mRNA is strongly induced in vitro by olive oil

691941

-

SDS

Malbranchea cinnamomea

A0A1S6YJF3

5%, 131% of initial activity

750134

sodium 2-{[(3alpha,5beta,12alpha)-3,12-dihydroxy-24-oxocholan-24-yl]amino}ethane-1-sulfonate

2 entries

sodium dioctyl sulfosuccinate

Fusarium solani

-

in the absence of surfactant the S54D variant catalytic activity is similar to that of the wild type cutinase, whereas L153Q and T179C variants show a lower activity

691090

Triton X-100

Malbranchea cinnamomea

A0A1S6YJF3

5%, 190% of initial activity

750134

Tween 20

Malbranchea cinnamomea

A0A1S6YJF3

5%, 118% of initial activity

750134

Tween 40

Malbranchea cinnamomea

A0A1S6YJF3

5%, 139.7% of initial activity

750134

Tween 60

Malbranchea cinnamomea

A0A1S6YJF3

5%, 139.5% of initial activity

750134

Tween 80

Malbranchea cinnamomea

A0A1S6YJF3

5%, 141.9% of initial activity

750134

additional information

7 entries

-

DMF

Thermobifida fusca

G8GER6, Q6A0I4

activates 10% at 40% v/v

729000

DMF

Thermobifida fusca

G8GER6, Q6A0I4

activates 6% at 40% v/v

729000

DMSO

Thermobifida fusca

G8GER6, Q6A0I4

activates 14% at 40% v/v

729000

DMSO

Thermobifida fusca

G8GER6, Q6A0I4

activates 9% at 40% v/v

729000

sodium 2-{[(3alpha,5beta,12alpha)-3,12-dihydroxy-24-oxocholan-24-yl]amino}ethane-1-sulfonate

Thermobifida fusca

G8GER6, Q6A0I4

activates 10% at 10 mM

729000

sodium 2-{[(3alpha,5beta,12alpha)-3,12-dihydroxy-24-oxocholan-24-yl]amino}ethane-1-sulfonate

Thermobifida fusca

G8GER6, Q6A0I4

activates 24% at 10 mM

729000

additional information

Fusarium solani

-

pseudo-activation in presence of sodium bis(2-ethylhexyl)ester sulfosuccinic acid and hexadecyltrimethyl-ammoniumbromide

208328

-

additional information

Fusarium solani

-

absence of interfacial activation

208329

-

additional information

Fusarium solani

-

no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket

709196

-

additional information

Fusarium solani

P00590

Pichia pastoris expressing the native non-tagged cutinase exhibits about 2- and 3fold higher values of protein amount and cutinase activity in the culture supernatant, respectively, than those containing the C-terminal tagged cutinase

710477

-

additional information

Fusarium solani

-

Pichia pastoris expressing the native non-tagged cutinase exhibits about 2- and 3fold higher values of protein amount and cutinase activity in the culture supernatant, respectively, than those containing the C-terminal tagged cutinase

710477

-

additional information

Fusarium solani

-

water:surfactant molar rate has a marked influence on the enzyme activity, with the best results in the range between 5 and 8. The use of detergents improves the reaction yield during wetting of cotton fibers. Increase in the stereoselectivity of the primary hydroxyl group acylation is obtained through the preincubation of the enzyme in the presence of the substrate diol 1, there is no correlation with the incubation time

707009

-

additional information

Thermobifida fusca

-

no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket

709196

-