3.1.1.52: phosphatidylinositol deacylase

This is an abbreviated version!
For detailed information about phosphatidylinositol deacylase, go to the full flat file.

Reaction

Synonyms

cPLA2alpha, group IVA cytosolic phospholipase A2alpha, phosphatidylinositol phospholipase A2, phospholipase A2, phosphatidylinositol

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.52 phosphatidylinositol deacylase

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Results	in table
4	Activating Compound
1	CAS Registry Number
1	Cloned(Commentary)
1	General Stability
5	Inhibitors
1	KM Value [mM]
4	Localization
3	Metals/Ions
3	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
5	Organism
1	Pathway
1	pH Optimum
1	pH Range
1	Posttranslational Modification
9	Protein Variants
2	Purification (Commentary)
1	Reaction
1	Reaction Type
5	Reference
5	Source Tissue
13	Specific Activity [micromol/min/mg]
12	Substrates and Products (Substrate)
1	Subunits
4	Synonyms
1	Systematic Name
1	Temperature Stability [°C]

Engineering

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Engineering on EC 3.1.1.52 - phosphatidylinositol deacylase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

K271N/K273N/K274N

Mus musculus

-

wild-type activity, half the wild-type increase in activity in presence of phosphatidylinositol 4,5-biphosphate

693262

K43N

Mus musculus

-

impaired release of arachidonic acid in response to serum and phorbol myristate acetate, no translocation to Golgi apparatus in response to serum, C2-domain mutant, calcium and membrane binding

693262

K488N/K543N/K544N

Mus musculus

-

2fold increased specific activity (independent of phosphatidylinositol 4,5-biphosphate), 78% reduced release of arachidonic acid and increased Golgi-binding in response to serum, PIP2-binding site triple mutant

693262

K541N/K543N/K544N

Mus musculus

-

2fold increased specific activity (independent of phosphatidylinositol 4,5-biphosphate)

693262

S505A

Mus musculus

-

38% reduced release of arachidonic acid and prolonged accumulation at Golgi apparatus in response to serum and 30% reduced release of arachidonic acid in response to phorbol myristate acetate, phosphorylation site mutant

693262

S505A/S727A

Mus musculus

-

30% reduced release of arachidonic acid in response to phorbol myristate acetate and 50% in response to serum which is further reduced by ERK inhibition, prolonged accumulation at Golgi apparatus in response to serum, phosphorylation site double mutant

693262

S505A/S727A/S515A

Mus musculus

-

similar response to serum as double mutant S505A/S727A, phosphorylation site triple mutant

693262

S515A

Mus musculus

-

phosphorylation site mutant

693262

S727A

Mus musculus

-

similar release of arachidonic acid in response to serum like wild-type enzyme, phosphorylation site mutant

693262