3.1.1.49: sinapine esterase

This is an abbreviated version!
For detailed information about sinapine esterase, go to the full flat file.

Word Map on EC 3.1.1.49

3.1.1.49

seedlings

brassica

napus

lipases

brassicaceae

antinutritive

sinapate

sinapoylmalate

Reaction

Synonyms

aromatic choline esterase, BnLIP2, BnSCE3, BnSCE3/BnLIP2, esterase, sinapine, SCE, sinapine esterase, sinapoylcholine(sinapine) esterase

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.1 Carboxylic-ester hydrolases

3.1.1.49 sinapine esterase

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Results	in table
1	Activating Compound
70	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
10	General Information
5	Inhibitors
26	KM Value [mM]
3	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
17	Organism
3	Pathway
1	pH Optimum
1	pH Range
1	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
14	Reference
12	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
37	Substrates and Products (Substrate)
1	Subunits
17	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]

Natural Substrates Products

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Natural Substrates Products on EC 3.1.1.49 - sinapine esterase

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O-sinapoylcholine + H2O

choline + sinapate

2 entries

sinapoylcholine + H2O

sinapic acid + choline

Brassica napus

Q3ZFI4

role for the GDSL lipase-like BnSCE3/BnLIP2 as a sinapine esterase, catalyzing hydrolysis of sinapine during seed germination, leading, via 1-O-sinapoyl-beta-glucose, to sinapoyl-L-malate in the seedlings

682385

additional information

Brassica napus

BnSCE3 reveals a broad substrate specificity toward phenolic choline esters, even accepting phosphatidylcholine, pointing to a phospholipase A-like activity

716651