3.1.1.35: dihydrocoumarin hydrolase

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For detailed information about dihydrocoumarin hydrolase, go to the full flat file.

3,4-dihydrocoumarin hydrolase, DCH

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Results	in table
2	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
2	Expression
13	Inhibitors
15	KM Value [mM]
5	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
23	Organism
3	Pathway
3	pH Optimum
3	pH Range
1	pH Stability
6	Purification (Commentary)
1	Reaction
1	Reaction Type
19	Reference
5	Source Tissue
7	Specific Activity [micromol/min/mg]
2	Storage Stability
69	Substrates and Products (Substrate)
4	Subunits
10	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification on EC 3.1.1.35 - dihydrocoumarin hydrolase

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89fold

ammonium sulfate, DEAE-Sephacel, Phenyl-Superose, Superdex 200

native enzyme from strain G27 186fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration